公开(公告)号：US6013864A

公开(公告)日：2000-01-11

申请号：US617454

申请日：1996-03-18

申请人： Timothy Albert Mitsky ,  Cynthia Lou Hemenway ,  Nilgun Ereken Tumer ,  Edgar Clifford Lawson

发明人： Timothy Albert Mitsky ,  Cynthia Lou Hemenway ,  Nilgun Ereken Tumer ,  Edgar Clifford Lawson

IPC分类号： C12N9/12 ,  C12N15/82 ,  A01H1/00 ,  C12N5/04 ,  C12N15/00

CPC分类号： C12N9/127 ,  C12N15/8283

摘要： An isolated DNA sequence which codes for a luteo replicase gene is disclosed herein. A method for providing resistance to infection by a virus by expressing a replicase gene in plants is also disclosed, as are transgenic plants containing the replicase gene.

摘要翻译： 本文公开了编码Luteo复制酶基因的分离的DNA序列。 还公开了通过在植物中表达复制酶基因来提供对病毒感染的抗性的方法，以及含有复制酶基因的转基因植物。

公开(公告)号：US5942660A

公开(公告)日：1999-08-24

申请号：US628039

申请日：1996-04-04

申请人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

发明人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

IPC分类号： C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/82 ,  C12P7/62 ,  A01H5/00 ,  C12N5/14 ,  C12N15/31 ,  C12N15/52

CPC分类号： C12N9/0006 ,  C12N15/8243 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254 ,  C12N9/00 ,  C12N9/1029 ,  C12N9/88 ,  C12P7/625

摘要： Genes and methods for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria via manipulation of normal metabolic pathways using recombinant DNA techniques are provided. This is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer end product. By these methods, enhanced levels of threonine, .alpha.-ketobutyrate, propionate, propionyl-CoA, .beta.-ketovaleryl-CoA, and .beta.-hydroxyvaleryl-CoA are produced. Also provided are methods for the biological production of P(3HB-co-3HV) copolymers in plants and bacteria utilizing propionyl-CoA produced through a variety of engineered metabolic pathways. Introduction into plants and bacteria of an appropriate .beta.-ketothiolase, .beta.-ketoacyl-CoA reductase, and PHA synthase, alone or in combination with various enzymes involved in asparate family amino acid biosynthesis and the conversion of threonine to PHA copolymer precursors, will permit these organisms to produce P(3HB-co-3HV) copolymers.

摘要翻译： 提供了通过使用重组DNA技术操作正常代谢途径来优化在植物和细菌中3-羟基丁酸酯（3HB）和3-羟基戊酸酯（3HV）的共聚物的生物合成中使用的底物水平的基因和方法。 这通过使用参与天冬氨酸家族氨基酸的生物合成的各种野生型和/或失调的酶以及涉及苏氨酸转化的野生型或去调节形式的酶，例如苏氨酸脱氨酶来实现 P（3HB-co-3HV）共聚物最终产物。 通过这些方法，产生了苏氨酸，α-酮丁酸酯，丙酸酯，丙酰基-CoA，β-酮戊酰-CoA和β-羟基戊酰-CoA的增强水平。 还提供了在植物和细菌中利用通过各种工程化代谢途径产生的丙酰辅酶A生物生产P（3HB-co-3HV）共聚物的方法。 单独或与参与天冬氨酸家族氨基酸生物合成的各种酶以及将苏氨酸转化为PHA共聚物前体的合适的β-酮硫解酶，β-酮酰基-CoA还原酶和PHA合酶的植物和细菌的引入将允许这些 生物体产生P（3HB-co-3HV）共聚物。

公开(公告)号：US5958745A

公开(公告)日：1999-09-28

申请号：US673388

申请日：1996-06-28

申请人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

发明人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/82 ,  C12P7/62 ,  A61K39/395 ,  C07K14/195

CPC分类号： C12N9/0006 ,  C12N15/8243 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254 ,  C12N9/00 ,  C12N9/1029 ,  C12N9/88 ,  C12P7/625

摘要： Genes and methods for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria via manipulation of normal metabolic pathways using recombinant DNA techniques are provided. This is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer endproduct. By these methods, enhanced levels of threonine, .alpha.-ketobutyrate, propionate, propionyl-CoA, .beta.-ketovaleryl-CoA, and .beta.-hydroxyvaleryl-CoA are produced. Also provided are methods for the biological production of P(3HB-co-3HV) copolymers in plants and bacteria utilizing propionyl-CoA produced through a variety of engineered metabolic pathways. Introduction into plants and bacteria of an appropriate .beta.-ketothiolase, .beta.-ketoacyl-CoA reductase, and PHA synthase, alone or in combination with various enzymes involved in asparate family amino acid biosynthesis and the conversion of threonine to PHA copolymer precursors, will permit these organisms to produce P(3HB-co-3HV) copolymers.

摘要翻译： 提供了通过使用重组DNA技术操作正常代谢途径来优化在植物和细菌中3-羟基丁酸酯（3HB）和3-羟基戊酸酯（3HV）的共聚物的生物合成中使用的底物水平的基因和方法。 这通过使用参与天冬氨酸家族氨基酸的生物合成的各种野生型和/或失调的酶以及涉及苏氨酸转化的野生型或去调节形式的酶，例如苏氨酸脱氨酶来实现 P（3HB-co-3HV）共聚物末端产物。 通过这些方法，产生了苏氨酸，α-酮丁酸酯，丙酸酯，丙酰基-CoA，β-酮戊酰-CoA和β-羟基戊酰-CoA的增强水平。 还提供了在植物和细菌中利用通过各种工程化代谢途径产生的丙酰辅酶A生物生产P（3HB-co-3HV）共聚物的方法。 单独或与参与天冬氨酸家族氨基酸生物合成的各种酶以及将苏氨酸转化为PHA共聚物前体的合适的β-酮硫解酶，β-酮酰基-CoA还原酶和PHA合成酶的植物和细菌的引入将允许这些 生物体产生P（3HB-co-3HV）共聚物。