公开(公告)号：US5942660A

公开(公告)日：1999-08-24

申请号：US628039

申请日：1996-04-04

申请人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

发明人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

IPC分类号： C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/82 ,  C12P7/62 ,  A01H5/00 ,  C12N5/14 ,  C12N15/31 ,  C12N15/52

CPC分类号： C12N9/0006 ,  C12N15/8243 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254 ,  C12N9/00 ,  C12N9/1029 ,  C12N9/88 ,  C12P7/625

摘要： Genes and methods for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria via manipulation of normal metabolic pathways using recombinant DNA techniques are provided. This is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer end product. By these methods, enhanced levels of threonine, .alpha.-ketobutyrate, propionate, propionyl-CoA, .beta.-ketovaleryl-CoA, and .beta.-hydroxyvaleryl-CoA are produced. Also provided are methods for the biological production of P(3HB-co-3HV) copolymers in plants and bacteria utilizing propionyl-CoA produced through a variety of engineered metabolic pathways. Introduction into plants and bacteria of an appropriate .beta.-ketothiolase, .beta.-ketoacyl-CoA reductase, and PHA synthase, alone or in combination with various enzymes involved in asparate family amino acid biosynthesis and the conversion of threonine to PHA copolymer precursors, will permit these organisms to produce P(3HB-co-3HV) copolymers.

摘要翻译： 提供了通过使用重组DNA技术操作正常代谢途径来优化在植物和细菌中3-羟基丁酸酯（3HB）和3-羟基戊酸酯（3HV）的共聚物的生物合成中使用的底物水平的基因和方法。 这通过使用参与天冬氨酸家族氨基酸的生物合成的各种野生型和/或失调的酶以及涉及苏氨酸转化的野生型或去调节形式的酶，例如苏氨酸脱氨酶来实现 P（3HB-co-3HV）共聚物最终产物。 通过这些方法，产生了苏氨酸，α-酮丁酸酯，丙酸酯，丙酰基-CoA，β-酮戊酰-CoA和β-羟基戊酰-CoA的增强水平。 还提供了在植物和细菌中利用通过各种工程化代谢途径产生的丙酰辅酶A生物生产P（3HB-co-3HV）共聚物的方法。 单独或与参与天冬氨酸家族氨基酸生物合成的各种酶以及将苏氨酸转化为PHA共聚物前体的合适的β-酮硫解酶，β-酮酰基-CoA还原酶和PHA合酶的植物和细菌的引入将允许这些 生物体产生P（3HB-co-3HV）共聚物。

公开(公告)号：US5958745A

公开(公告)日：1999-09-28

申请号：US673388

申请日：1996-06-28

申请人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

发明人： Kenneth James Gruys ,  Timothy Albert Mitsky ,  Ganesh Murthy Kishore ,  Steven Charles Slater ,  Stephen Rogers Padgette ,  David Martin Stark

IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/82 ,  C12P7/62 ,  A61K39/395 ,  C07K14/195

CPC分类号： C12N9/0006 ,  C12N15/8243 ,  C12N15/8251 ,  C12N15/8253 ,  C12N15/8254 ,  C12N9/00 ,  C12N9/1029 ,  C12N9/88 ,  C12P7/625

摘要： Genes and methods for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants and bacteria via manipulation of normal metabolic pathways using recombinant DNA techniques are provided. This is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer endproduct. By these methods, enhanced levels of threonine, .alpha.-ketobutyrate, propionate, propionyl-CoA, .beta.-ketovaleryl-CoA, and .beta.-hydroxyvaleryl-CoA are produced. Also provided are methods for the biological production of P(3HB-co-3HV) copolymers in plants and bacteria utilizing propionyl-CoA produced through a variety of engineered metabolic pathways. Introduction into plants and bacteria of an appropriate .beta.-ketothiolase, .beta.-ketoacyl-CoA reductase, and PHA synthase, alone or in combination with various enzymes involved in asparate family amino acid biosynthesis and the conversion of threonine to PHA copolymer precursors, will permit these organisms to produce P(3HB-co-3HV) copolymers.

摘要翻译： 提供了通过使用重组DNA技术操作正常代谢途径来优化在植物和细菌中3-羟基丁酸酯（3HB）和3-羟基戊酸酯（3HV）的共聚物的生物合成中使用的底物水平的基因和方法。 这通过使用参与天冬氨酸家族氨基酸的生物合成的各种野生型和/或失调的酶以及涉及苏氨酸转化的野生型或去调节形式的酶，例如苏氨酸脱氨酶来实现 P（3HB-co-3HV）共聚物末端产物。 通过这些方法，产生了苏氨酸，α-酮丁酸酯，丙酸酯，丙酰基-CoA，β-酮戊酰-CoA和β-羟基戊酰-CoA的增强水平。 还提供了在植物和细菌中利用通过各种工程化代谢途径产生的丙酰辅酶A生物生产P（3HB-co-3HV）共聚物的方法。 单独或与参与天冬氨酸家族氨基酸生物合成的各种酶以及将苏氨酸转化为PHA共聚物前体的合适的β-酮硫解酶，β-酮酰基-CoA还原酶和PHA合成酶的植物和细菌的引入将允许这些 生物体产生P（3HB-co-3HV）共聚物。

公开(公告)号：US5804425A

公开(公告)日：1998-09-08

申请号：US833485

申请日：1997-04-07

申请人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

发明人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

IPC分类号： C12N9/10 ,  C12N15/54 ,  C12N15/82 ,  C12N1/00 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/1092 ,  C12N15/8275 ,  Y10S435/816 ,  Y10S435/822 ,  Y10S435/839 ,  Y10S435/874 ,  Y10S435/883 ,  Y10T436/143333

摘要： Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.

公开(公告)号：US20080227966A1

公开(公告)日：2008-09-18

申请号：US11526170

申请日：2006-09-22

申请人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

发明人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

IPC分类号： C07H21/04

CPC分类号： C12N9/1092 ,  C12N15/8275 ,  Y10S435/816 ,  Y10S435/822 ,  Y10S435/839 ,  Y10S435/874 ,  Y10S435/883 ,  Y10T436/143333

摘要： Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.

摘要翻译： 公开了编码II类EPSPS酶的基因。 这些基因可用于产生耐草甘膦除草剂的转化细菌和植物。 II类EPSPS基因与已知的I类EPSPS基因几乎没有同源性，并且不与I类EPSPS的探针杂交。 II类EPSPS酶的特征在于在草甘膦存在下比I类EPSPS更具动力学效力。 还公开了用II类EPSPS基因转化的植物以及在种植的转基因作物田中选择性控制杂草的方法。

公开(公告)号：US06248876B1

公开(公告)日：2001-06-19

申请号：US09137440

申请日：1998-08-20

申请人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

发明人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

IPC分类号： C07H2104

CPC分类号： C12N9/1092 ,  C12N15/8275 ,  Y10S435/816 ,  Y10S435/822 ,  Y10S435/839 ,  Y10S435/874 ,  Y10S435/883 ,  Y10T436/143333

摘要： Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.

摘要翻译： 公开了编码II类EPSPS酶的基因。 这些基因可用于产生耐草甘膦除草剂的转化细菌和植物。 II类EPSPS基因与已知的I类EPSPS基因几乎没有同源性，并且不与I类EPSPS的探针杂交。 II类EPSPS酶的特征在于在草甘膦存在下比I类EPSPS更具动力学效力。 还公开了用II类EPSPS基因转化的植物以及在种植的转基因作物田中选择性控制杂草的方法。

公开(公告)号：US20100022762A1

公开(公告)日：2010-01-28

申请号：US12459672

申请日：2009-07-06

申请人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

发明人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

IPC分类号： C07H21/04

CPC分类号： C12N9/1092 ,  C12N15/8275 ,  Y10S435/816 ,  Y10S435/822 ,  Y10S435/839 ,  Y10S435/874 ,  Y10S435/883 ,  Y10T436/143333

摘要： Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.

摘要翻译： 公开了编码II类EPSPS酶的基因。 这些基因可用于产生耐草甘膦除草剂的转化细菌和植物。 II类EPSPS基因与已知的I类EPSPS基因几乎没有同源性，并且不与I类EPSPS的探针杂交。 II类EPSPS酶的特征在于在草甘膦存在下比I类EPSPS更具动力学效力。 还公开了用II类EPSPS基因转化的植物以及在种植的转基因作物田中选择性控制杂草的方法。

公开(公告)号：US07183110B2

公开(公告)日：2007-02-27

申请号：US09464099

申请日：1999-12-16

申请人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

发明人： Gerard Francis Barry ,  Ganesh Murthy Kishore ,  Stephen Rogers Padgette ,  William Carlton Stallings

IPC分类号： C12N1/00 ,  C07K16/00

CPC分类号： C12N9/1092 ,  C12N15/8275 ,  Y10S435/816 ,  Y10S435/822 ,  Y10S435/839 ,  Y10S435/874 ,  Y10S435/883 ,  Y10T436/143333

摘要： Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to probes from Class I EPSPS's. The Class II EPSPS enzymes are characterized by being more kinetically efficient than Class I EPSPS's in the presence of glyphosate. Plants transformed with Class II EPSPS genes are also disclosed as well as a method for selectively controlling weeds in a planted transgenic crop field.

摘要翻译： 公开了编码II类EPSPS酶的基因。 这些基因可用于产生耐草甘膦除草剂的转化细菌和植物。 II类EPSPS基因与已知的I类EPSPS基因几乎没有同源性，并且不与I类EPSPS的探针杂交。 II类EPSPS酶的特征在于在草甘膦存在下比I类EPSPS更具动力学效力。 还公开了用II类EPSPS基因转化的植物以及在种植的转基因作物田中选择性控制杂草的方法。