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公开(公告)号：US07244603B2
公开(公告)日：2007-07-17
申请号：US10796669
申请日：2004-03-09
Applicant: Jin-Town Wang , Tzu-Lung Lin
Inventor： Jin-Town Wang , Tzu-Lung Lin
Abstract: Disclosed is a novel type II restriction endonuclease. Such enzyme recognizes a particular non-palindromic sequence of 5 oligonucleotides and cleaves DNA downstream of the DNA recognition sequence of nucleotides at the fourth base in the upper strand and the fifth base in the lower strand, and forms a one-base protruding end in the 5′-end after cleavage. The recognition and cleavage site of HpyC1I is identical to the known restriction endonuclease BccI respectively, but the nucleotide sequence and the amino acid sequence are different from any other know restriction enzymes.
Abstract translation: 公开了一种新型的II型限制性内切核酸酶。 这种酶识别5个寡核苷酸的特定非回文序列，并在上链第4碱基和下链第5碱基的DNA识别序列的DNA识别序列下游切割DNA，并形成在 切割后5'末端。 HpyC1I的识别和切割位点分别与已知的限制性内切核酸酶BccI相同，但核苷酸序列和氨基酸序列与任何其他已知的限制酶不同。
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公开(公告)号：US20050202443A1
公开(公告)日：2005-09-15
申请号：US10796669
申请日：2004-03-09
Applicant: Jin-Town Wang , Tzu-Lung Lin
Inventor： Jin-Town Wang , Tzu-Lung Lin
Abstract: Disclosed is a novel type II restriction endonuclease. Such enzyme recognizes a particular non-palindromic sequence of 5 oligonucleotides and cleaves DNA downstream of the DNA recognition sequence of nucleotides at the fourth base in the upper strand and the fifth base in the lower strand, and forms a one-base protruding end in the 5′-end after cleavage. The recognition and cleavage site of HpyC1I is identical to the known restriction endonuclease BccI respectively, but the nucleotide sequence and the amino acid sequence are different from any other know restriction enzymes.
Abstract translation: 公开了一种新型的II型限制性内切核酸酶。 这种酶识别5个寡核苷酸的特定非回文序列，并在上链第4碱基和下链第5碱基的DNA识别序列的DNA识别序列下游切割DNA，并形成在 切割后5'末端。 HpyC1I的识别和切割位点分别与已知的限制性内切核酸酶BccI相同，但核苷酸序列和氨基酸序列与任何其他已知的限制酶不同。
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3.发明授权PCR method for identifiying a serotype of Klebsiella pneumoniae by using cps region primers and application thereof 有权通过使用cps区域引物鉴定肺炎克雷伯杆菌血清型的PCR方法及其应用公开(公告)号：US08110359B2
公开(公告)日：2012-02-07
申请号：US12424062
申请日：2009-04-15
Applicant: Jin-Town Wang , Yi-Jiun Pan , Han-Chi Fang , Hui-Ching Yang , Tzu-Lung Lin , Pei-Fang Hsieh , Feng-Chiao Tsai , Yoav Keynan
Inventor： Jin-Town Wang , Yi-Jiun Pan , Han-Chi Fang , Hui-Ching Yang , Tzu-Lung Lin , Pei-Fang Hsieh , Feng-Chiao Tsai , Yoav Keynan
CPC classification number: C12Q1/689
Abstract: The present invention relates to a method of identifying a serotype of Klebsiella pneumoniae, in particular to a method using specific polymerase chain reaction (PCR) primer sets designed according to a fragment of a capsular polysaccharide synthesis (cps) region to identify a K57 or a NTUH-N1 serotype and its application. NTUH-N1 is a novel serotype which differs from the previously reported 77 serotypes. This PCR-based cps genotyping method not only solves the problems of insufficient specificity and sensitivity caused by conventional immune method, but can be applied in clinical diagnosis with the advantages of rapidity and low cost. In addition, the rate of unidentifiable strains can also be reduced by this method.
Abstract translation: 本发明涉及鉴定肺炎克雷伯杆菌血清型的方法，特别涉及使用根据荚膜多糖合成（cps）区域的片段设计的特异性聚合酶链反应（PCR）引物组的方法，以鉴定K57或 NTUH-N1血清型及其应用。 NTUH-N1是一种新型血清型，与先前报道的77种血清型不同。 这种基于PCR的cps基因分型方法不仅解决了常规免疫方法引起的特异性和敏感性不足的问题，而且可以快速，低成本的优点应用于临床诊断。 另外，这种方法也可以减少不可识别的菌株的比例。
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4.发明申请Method of exposure compensation for simulating the dynamic range of human eyes 审中-公开用于模拟人眼动态范围的曝光补偿方法公开(公告)号：US20050276476A1
公开(公告)日：2005-12-15
申请号：US10863222
申请日：2004-06-09
Applicant: Tzu-Lung Lin
Inventor： Tzu-Lung Lin
Abstract: A method of exposure compensation for simulating the dynamic of human eye comprises the following steps: sampling the brightness of an image; plotting a brightness level graph; converting the brightness level graph into a histogram of brightness; converting the histogram of brightness into a percentile diagram of brightness; converting the percentile diagram of brightness into a diagram of gamut curve; and replacing the gamut curve of the original image with the new gamut curve to obtain a modified image. Thereby, color level of the large part of low brightness can be raised to show the details of the image, and an excellent image quality is available.
Abstract translation: 一种用于模拟人眼动态的曝光补偿方法包括以下步骤：对图像的亮度进行采样; 绘制亮度水平图; 将亮度水平图转换为亮度直方图; 将亮度直方图转换为亮度百分位图; 将亮度的百分位图转换为色域曲线图; 并用新的色域曲线替换原始图像的色域曲线以获得修改的图像。 因此，可以提高大部分低亮度的色阶以显示图像的细节，并且可以获得优异的图像质量。
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公开(公告)号：US09617314B2
公开(公告)日：2017-04-11
申请号：US14112870
申请日：2012-04-20
Applicant: Jin Town Wang , Tzu Lung Lin
Inventor： Jin Town Wang , Tzu Lung Lin
IPC: C07K14/005 , C12N7/00 , C07H21/04
CPC classification number: C12N7/00 , C07H21/04 , C07K14/005 , C12N2795/00021 , C12N2795/00022 , C12N2795/00032 , C12N2795/00033 , C12N2795/10221 , C12N2795/10222 , C12N2795/10232 , C12N2795/10233 , C12N2795/10322
Abstract: The present invention relates to novel bacteriophages specific to Klebsiella pneumoniae strains, and compositions comprising the same. Particularly, polypeptides and their coding nucleic acid molecule of the novel bacteriophages are provided. The invention also relates to applications of the novel bacteriophages and the polypeptides in the detection/treatment/prevention of infection caused by Klebsiella pneumoniae strains. Development of immunogen and vaccine on the basis of the polypeptides are also provided.
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Patent Agency Ranking
6.发明申请NOVELPOLYPEPTIDES AND BACTERIOPHAGES SPECIFIC TO KLEBSIELLA PNEUMONIAE CAPSULAR TYPE STRAINS 有权克氏菌属囊状菌株特异性蛋白酶和细菌特异性公开(公告)号：US20140295528A1
公开(公告)日：2014-10-02
申请号：US14112870
申请日：2012-04-20
Applicant: Jin Town Wang , Tzu Lung Lin
Inventor： Jin Town Wang , Tzu Lung Lin
IPC: C07K14/005 , C12N7/00
CPC classification number: C12N7/00 , C07H21/04 , C07K14/005 , C12N2795/00021 , C12N2795/00022 , C12N2795/00032 , C12N2795/00033 , C12N2795/10221 , C12N2795/10222 , C12N2795/10232 , C12N2795/10233 , C12N2795/10322
Abstract: The present invention relates to novel bacteriophages specific to Klebsiella pneumoniae strains, and compositions comprising the same. Particularly, polypeptides and their coding nucleic acid molecule of the novel bacteriophages are provided. The invention also relates to applications of the novel bacteriophages and the polypeptides in the detection/treatment/prevention of infection caused by Klebsiella pneumoniae strains. Development of immunogen and vaccine on the basis of the polypeptides are also provided.
Abstract translation: 本发明涉及对肺炎克雷伯杆菌菌株特异性的新型噬菌体及其组合物。 特别地，提供了新型噬菌体的多肽及其编码核酸分子。 本发明还涉及新型噬菌体和多肽在检测/治疗/预防由肺炎克雷伯菌引起的感染中的应用。 还提供了基于多肽的免疫原和疫苗的开发。
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7.发明申请METHOD OF IDENTIFIYING A SEROTYPE OF KLEBSIELLA PNEUMONIAE AND APPLICATION THEREOF 有权鉴定KlebsiellA PNEUMONIAE及其应用的方法公开(公告)号：US20100136534A1
公开(公告)日：2010-06-03
申请号：US12424062
申请日：2009-04-15
Applicant: Jin-Town Wang , Yi-Jiun Pan , Han-Chi Fang , Hui-Ching Yang , Tzu-Lung Lin , Pei-Fang Hsieh , Feng-Chiao Tsai , Yoav Keynan
Inventor： Jin-Town Wang , Yi-Jiun Pan , Han-Chi Fang , Hui-Ching Yang , Tzu-Lung Lin , Pei-Fang Hsieh , Feng-Chiao Tsai , Yoav Keynan
CPC classification number: C12Q1/689
Abstract: The present invention relates to a method of identifying a serotype of Klebsiella pneumoniae, in particular to a method using specific polymerase chain reaction (PCR) primer sets designed according to a fragment of a capsular polysaccharide synthesis (cps) region to identify a K57 or a NTUH-N1 serotype and its application. NTUH-N1 is a novel serotype which differs from the previously reported 77 serotypes. This PCR-based cps genotyping method not only solves the problems of insufficient specificity and sensitivity caused by conventional immune method, but can be applied in clinical diagnosis with the advantages of rapidity and low cost. In addition, the rate of unidentifiable strains can also be reduced by this method.
Abstract translation: 本发明涉及鉴定肺炎克雷伯杆菌血清型的方法，特别涉及使用根据荚膜多糖合成（cps）区域的片段设计的特异性聚合酶链反应（PCR）引物组的方法，以鉴定K57或 NTUH-N1血清型及其应用。 NTUH-N1是一种新型血清型，与先前报道的77种血清型不同。 这种基于PCR的cps基因分型方法不仅解决了常规免疫方法引起的特异性和敏感性不足的问题，而且可以快速，低成本的优点应用于临床诊断。 另外，这种方法也可以减少不可识别的菌株的比例。
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8.发明申请KLEBSIELLA PNEUMONIAE ATTENUATED VIRULENCE MUTANT AND METHOD OF PRODUCTION 审中-公开KLEBSIELLA PNEUMONIAE衰减的VIRULANT突变体和生产方法公开(公告)号：US20100129408A1
公开(公告)日：2010-05-27
申请号：US12434520
申请日：2009-05-01
Applicant: Jin-Town Wang , Pei-Fang Hsieh , Tzu-Lung Lin , Cha-Ze Lee , Shih-Feng Tsai
Inventor： Jin-Town Wang , Pei-Fang Hsieh , Tzu-Lung Lin , Cha-Ze Lee , Shih-Feng Tsai
IPC: A61K39/108 , C12N1/20 , C12Q1/68
CPC classification number: C12N1/36 , A61K39/0266 , A61K2039/522 , C12N1/20 , C12Q1/689
Abstract: The present invention relates to a Klebsiella pneumoniae gene-deleted mutant and its producing methods. The main objective of this invention is to construct a Klebsiella pneumoniae tonB deletion mutant used as an immunogenically effective amount of a live attenuated vaccine against the community-acquired pyogenic liver abscess, where the tonB gene was deleted. The tonB deletion mutant maintained the structure of the capsular polysaccharide on cell membrane surface, and induced no disease on the mouse after injection. In addition, the tonB deletion mutants induced the immunity against pyogenic liver abscess caused by Klebsiella pneumoniae.
Abstract translation: 本发明涉及肺炎克雷伯杆菌基因缺失突变体及其制备方法。 本发明的主要目的是构建肺炎克雷伯杆菌tonB缺失突变体，其用作免疫有效量的针对社区获得性化脓性肝脓肿的活减毒疫苗，其中tonB基因被缺失。 tonB缺失突变体保持细胞膜表面上的荚膜多糖的结构，注射后不会引起小鼠的疾病。 此外，tonB缺失突变体诱导了由肺炎克雷伯氏杆菌引起的化脓性肝脓肿免疫。
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公开(公告)号：USD409775S
公开(公告)日：1999-05-11
申请号：US93556
申请日：1998-09-14
Applicant: Tzu-Lung Lin
Designer： Tzu-Lung Lin
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公开(公告)号：US5690410A
公开(公告)日：1997-11-25
申请号：US553774
申请日：1995-10-23
Applicant: Tzu-Lung Lin
Inventor： Tzu-Lung Lin
Abstract: A light device includes a sensor secured to the fork of a bicycle and an inducing block secured to the wheel for moving pass the sensor so as to actuate the sensor. A housing is securing to the bicycle for receiving a microprocessor which is coupled to the sensor. A displayer is coupled to the microprocessor for displaying a speed of the bicycle. Two light bulbs of different wattages are coupled to the microprocessor so as to be energized when the inducing block moves pass the sensor. A switch is coupled to the microprocessor for controlling the lighting modes.
Abstract translation: 灯装置包括固定到自行车的叉子的传感器和固定到车轮上的诱导块，用于移动传递传感器以致动传感器。 外壳固定在自行车上，用于接收耦合到传感器的微处理器。 显示器耦合到微处理器以显示自行车的速度。 不同功率的两个灯泡耦合到微处理器，以便当感应块移动通过传感器时被激励。 开关耦合到微处理器以控制照明模式。
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