PCR method for identifiying a serotype of Klebsiella pneumoniae by using cps region primers and application thereof
    2.
    发明授权
    PCR method for identifiying a serotype of Klebsiella pneumoniae by using cps region primers and application thereof 有权
    通过使用cps区域引物鉴定肺炎克雷伯杆菌血清型的PCR方法及其应用

    公开(公告)号:US08110359B2

    公开(公告)日:2012-02-07

    申请号:US12424062

    申请日:2009-04-15

    CPC classification number: C12Q1/689

    Abstract: The present invention relates to a method of identifying a serotype of Klebsiella pneumoniae, in particular to a method using specific polymerase chain reaction (PCR) primer sets designed according to a fragment of a capsular polysaccharide synthesis (cps) region to identify a K57 or a NTUH-N1 serotype and its application. NTUH-N1 is a novel serotype which differs from the previously reported 77 serotypes. This PCR-based cps genotyping method not only solves the problems of insufficient specificity and sensitivity caused by conventional immune method, but can be applied in clinical diagnosis with the advantages of rapidity and low cost. In addition, the rate of unidentifiable strains can also be reduced by this method.

    Abstract translation: 本发明涉及鉴定肺炎克雷伯杆菌血清型的方法,特别涉及使用根据荚膜多糖合成(cps)区域的片段设计的特异性聚合酶链反应(PCR)引物组的方法,以鉴定K57或 NTUH-N1血清型及其应用。 NTUH-N1是一种新型血清型,与先前报道的77种血清型不同。 这种基于PCR的cps基因分型方法不仅解决了常规免疫方法引起的特异性和敏感性不足的问题,而且可以快速,低成本的优点应用于临床诊断。 另外,这种方法也可以减少不可识别的菌株的比例。

    METHOD OF IDENTIFIYING A SEROTYPE OF KLEBSIELLA PNEUMONIAE AND APPLICATION THEREOF
    4.
    发明申请
    METHOD OF IDENTIFIYING A SEROTYPE OF KLEBSIELLA PNEUMONIAE AND APPLICATION THEREOF 有权
    鉴定KlebsiellA PNEUMONIAE及其应用的方法

    公开(公告)号:US20100136534A1

    公开(公告)日:2010-06-03

    申请号:US12424062

    申请日:2009-04-15

    CPC classification number: C12Q1/689

    Abstract: The present invention relates to a method of identifying a serotype of Klebsiella pneumoniae, in particular to a method using specific polymerase chain reaction (PCR) primer sets designed according to a fragment of a capsular polysaccharide synthesis (cps) region to identify a K57 or a NTUH-N1 serotype and its application. NTUH-N1 is a novel serotype which differs from the previously reported 77 serotypes. This PCR-based cps genotyping method not only solves the problems of insufficient specificity and sensitivity caused by conventional immune method, but can be applied in clinical diagnosis with the advantages of rapidity and low cost. In addition, the rate of unidentifiable strains can also be reduced by this method.

    Abstract translation: 本发明涉及鉴定肺炎克雷伯杆菌血清型的方法,特别涉及使用根据荚膜多糖合成(cps)区域的片段设计的特异性聚合酶链反应(PCR)引物组的方法,以鉴定K57或 NTUH-N1血清型及其应用。 NTUH-N1是一种新型血清型,与先前报道的77种血清型不同。 这种基于PCR的cps基因分型方法不仅解决了常规免疫方法引起的特异性和敏感性不足的问题,而且可以快速,低成本的优点应用于临床诊断。 另外,这种方法也可以减少不可识别的菌株的比例。

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