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9 条记录 (耗时 0.024 秒)

    Enzymatic process for preparing N-acetylneuraminic acid
    1.
    发明授权
    Enzymatic process for preparing N-acetylneuraminic acid 失效
    用于制备N-乙酰氨基酸的酶法

    公开(公告)号：US5071750A

    公开(公告)日：1991-12-10

    申请号：US606944

    申请日：1990-10-31

    申请人： Udo Kragl ,  Christian Wandrey ,  Oreste Ghisalba ,  Daniel Gygax

    发明人： Udo Kragl ,  Christian Wandrey ,  Oreste Ghisalba ,  Daniel Gygax

    IPC分类号： C12P19/26

    CPC分类号： C12P19/26

    摘要： A process for obtaining N-acetylneuraminic acid from N-acetylglucosamine is disclosed. The process is carried out in a reactor which contains both N-acylglucosamine-2-epimerase (E.C. 5.1.3.8) which isomerizes GlcNAc into ManNAc, and N-acetylneuraminic acid pyruvate lyase (E.C. 4.1.3.3) which catalyzes the reaction of the resulting ManNAc with pyruvic acid to give Neu5Ac. GlcNAc and Pyr are fed into the reactor and Neu5Ac is obtained in the outflow. The process is preferably carried out continuously and in particular in an enzyme membrane reactor at pH 7.5 and 25.degree. C., especially using residence times of 0.2 to 10 h, and with an excess of GlcNAc in comparison with Pyr which is subsequently added if necessary. Epimerase and lyase are preferably present in the reactor in a ratio of activities which is equivalent to the reciprocal value of the quotient of the conversion rates.

    摘要翻译： 公开了从N-乙酰葡糖胺获得N-乙酰神经氨酸的方法。 该方法在含有将GlcNac异构化成ManNac的N-酰基葡糖胺-2-差向异构酶（EC 5.1.3.8）和N-乙酰神经氨酸丙酮酸裂合酶（EC 4.1.3.3）的反应器中进行，其催化所得到的 ManNac与丙酮酸反应得到Neu5Ac。 将GlcNAc和Pyr进料到反应器中，并在流出物中获得Neu5Ac。 该方法优选连续地，特别是在pH 7.5和25℃的酶膜反应器中进行，特别是使用0.2至10小时的停留时间，以及与Pyr相比过量的GlcNac，如果需要，随后加入 。 差向异构酶和裂解酶优选以相当于转化率的商的倒数值的活性比例存在于反应器中。

    Phosphorus containing cyclic nucleotides
    5.
    发明授权
    Phosphorus containing cyclic nucleotides 失效
    含磷环的核苷酸

    公开(公告)号：US06340754B1

    公开(公告)日：2002-01-22

    申请号：US09664596

    申请日：2000-09-18

    申请人： Oreste Ghisalba ,  Guy Joel Christian Marais ,  Pierre Martin

    发明人： Oreste Ghisalba ,  Guy Joel Christian Marais ,  Pierre Martin

    IPC分类号： C07F906

    CPC分类号： C07H19/06 ,  C07F9/65744 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20

    摘要： The invention relates to a compound of formula (I) Wherein: B is adenine, guanine or hypoxanthine Z is hydrogen or a negative charge R is —[CH2CH(R1)—O]n—R2, —CH2CH2X, in which R1 is hydrogen or (C1-C6) alkyl R2 is hydrogen or (C1-C6) alkyl n is a number from 1 to 6 X is OH, F, NR3R4 R3 and R4 are independently from each other hydrogen or (C1-C6) alkyl and to methods of enzymatically treating these compounds with biocatalysts having cyclic phosphodiesterase activity.

    摘要翻译： 本发明涉及式（I）化合物，其中B是腺嘌呤，鸟嘌呤或次黄嘌呤Z是氢或负电荷R是 - [CH2CH（R1）-O] n -R2，-CH2CH2X，其中R1是氢或（C1 -C 6）烷基R 2是氢或（C 1 -C 6）烷基是1至6X的数，OH，F，NR 3 R 4 R 3和R 4彼此独立地为氢或（C 1 -C 6）烷基，并且与 具有环状磷酸二酯酶活性的生物催化剂。

    Microbial process for the preparation of milbemycin derivatives
    6.
    发明授权
    Microbial process for the preparation of milbemycin derivatives 失效
    微生物制备米尔倍霉素衍生物的方法

    公开(公告)号：US5183749A

    公开(公告)日：1993-02-02

    申请号：US496183

    申请日：1990-03-20

    申请人： Gerado Ramos ,  Oreste Ghisalba ,  Hans-Peter Schar ,  Bruno Frei ,  Peter Maienfisch ,  Anthony C. O'Sullivan

    发明人： Gerado Ramos ,  Oreste Ghisalba ,  Hans-Peter Schar ,  Bruno Frei ,  Peter Maienfisch ,  Anthony C. O'Sullivan

    IPC分类号： A01N49/00 ,  C07H19/01 ,  C12P17/18

    CPC分类号： C07H19/01 ,  A01N49/00 ,  C12P17/181 ,  C12R1/525 ,  C12R1/685 ,  Y10S435/822 ,  Y10S435/886 ,  Y10S435/911 ,  Y10S435/917

    摘要： The present invention relates to a one-step microbial process for the preparation of 13.beta.-hydroxy- or 14,15-epoxy-milbemycins of the following formulae I and II ##STR1## in which R.sub.1 and R.sub.1 ' represent methyl, ethyl, isopropyl or sec.-butyl or represent the group ##STR2## in which X represents methyl, ethyl or isopropyl, and A and A' represent the group ##STR3## in which R.sub.2 represents hydrogen, --C(O)-CH.sub.2 O-C(O)-CH.sub.2 CH.sub.2 -COOH or --C(O)-CH.sub.2 CH.sub.2 -COOH, or mixtures of compounds of the formulae I and II, which process comprises bringing a milbemycin of the formula III ##STR4## dissolved in a liquid phase, in which R.sub.3 represents R.sub.1 or R.sub.1 ' and R.sub.4 represents A or A', and R.sub.1, R.sub.1 ', A and A' have the meanings given for formulae I and II, into contact with a microorganism that is capable of 13.beta.-hydroxylation or 14,15-epoxidation or of both reactions, or into contact with active constituents thereof. The microorganism or its active constituents may be either in free form or immobilized form.

    摘要翻译： 本发明涉及一种用于制备下式I和II的13β-羟基或14,15-环氧 - 米尔倍霉素的一步微生物方法。其中R1 并且R 1'表示甲基，乙基，异丙基或仲丁基，或表示其中X表示甲基，乙基或异丙基的基团，A和A'表示其中R 2表示氢的基团，-C （O）-CH 2 O-C（O）-CH 2 CH 2 -COOH或-C（O）-CH 2 CH 2 -COOH，或式I和II化合物的混合物，该方法包括使式III的米尔倍霉素 III）溶解在液相中，其中R3表示R1或R1'，R4表示A或A'，R1，R1'，A和A'具有式I和II给出的含义，与微生物接触， 能够进行13β-羟基化或14,15-环氧化或两种反应，或与其活性成分接触。 微生物或其活性成分可以是游离形式或固定化形式。

    Microorganisms of the genus Hyphomicrobium and process for degrading compounds wich contain methyl groups in aqueous solutions
    8.
    发明授权
    Microorganisms of the genus Hyphomicrobium and process for degrading compounds wich contain methyl groups in aqueous solutions 失效
    微生物的微生物和在水溶液中含有甲基的降解化合物的方法

    公开(公告)号：US4492756A

    公开(公告)日：1985-01-08

    申请号：US445783

    申请日：1982-12-01

    申请人： Oreste Ghisalba ,  Franz Heinzer ,  Martin Kuenzi

    发明人： Oreste Ghisalba ,  Franz Heinzer ,  Martin Kuenzi

    IPC分类号： C02F3/34 ,  C12N1/20 ,  C12N1/32 ,  C07 ,  C12R1/01

    CPC分类号： C12R1/01 ,  C02F3/34 ,  C12N1/20 ,  C12N1/32 ,  Y10S435/822

    摘要： The present invention relates to novel facultative methylotrophic microorganisms of the genus Hyphomicrobium, to protein-containing biomass, and to a process for the microbiological purification of aqueous solutions, e.g. wastewaters, which contain methanol, ethanol, glucose, dimethyl phosphite, trimethyl phosphite, sodium formate, sodium acetate, methylammonium chloride, dimethylammonium chloride, ethylmethylammonium chloride or, chiefly, sodium methyl sulfate, as pollutants. The respective microorganism is cultured in aqueous solution and the pollutant is degraded simultaneously.

    摘要翻译： 本发明涉及新型兼性微营养微生物，涉及含蛋白质的生物质，以及涉及用于微生物学纯化水溶液的方法。 含有甲醇，乙醇，葡萄糖，亚磷酸二甲酯，亚磷酸三甲酯，甲酸钠，乙酸钠，甲基氯化铵，二甲基氯化铵，乙基甲基氯化铵或主要是甲基硫酸钠作为污染物的废水。 将各微生物在水溶液中培养，同时污染物降解。

    Biocatalysts with amine acylase activity
    9.
    发明授权
    Biocatalysts with amine acylase activity 失效
    具有胺酰基转移酶活性的生物催化剂

    公开(公告)号：US06235516B1

    公开(公告)日：2001-05-22

    申请号：US09171646

    申请日：1998-11-12

    申请人： Oreste Ghisalba ,  Matthias Kittelmann ,  Kurt Laumen ,  Paula Walser-Volken

    发明人： Oreste Ghisalba ,  Matthias Kittelmann ,  Kurt Laumen ,  Paula Walser-Volken

    IPC分类号： C12N978

    CPC分类号： C12N9/80 ,  C12P41/007 ,  Y10S435/826 ,  Y10S435/893 ,  Y10S435/908

    摘要： The invention belongs to the field of biotechnology. It concerns a biocatalyst, i.e. a dead or living microorganism or a polypeptide, preferably in isolated form, which exhibits acylase enzymatic activity without lipase- or esterase-activity. The biocatalyst is capable of stereoselectively hydrolysing a racemic acylamide which has an aliphatic acyl residue and which is not a derivative of a natural amino acid.

    摘要翻译： 本发明属于生物技术领域。 它涉及生物催化剂，即死亡或活的微生物或多肽，优选分离形式，其显示没有脂肪酶或酯酶活性的酰基转移酶酶活性。 该生物催化剂能够立体选择性水解具有脂肪族酰基残基并且不是天然氨基酸衍生物的外消旋酰基酰胺。