公开(公告)号：US07514540B2

公开(公告)日：2009-04-07

申请号：US10451652

申请日：2001-12-03

申请人： Pablo Wessig ,  Jürgen Bendig ,  Uwe Schedler

发明人： Pablo Wessig ,  Jürgen Bendig ,  Uwe Schedler

IPC分类号： C07K1/00 ,  C07K17/14 ,  C07K17/10 ,  C07K17/08 ,  C07K17/06 ,  C12N11/14 ,  C12N11/10 ,  C12N11/06

CPC分类号： C07K17/06 ,  C07K1/1072 ,  Y10S530/811 ,  Y10S530/813 ,  Y10S530/816

摘要： The invention concerns a method for immobilizing biomolecules on a polymeric surface of a carrier material and a carrier material functionalized with a biomolecule according to the general formulas (IV) and (V), which can be produced according to the method, wherein P indicates the polymeric surface of the carrier material and M indicates the biomolecule bound to the linker compound via an amino group (formula IV) or a carbonyl group (formula V) and R2 has the meaning OR4 or NR4R5; R1, R4 and R5, independently of one another, indicate H, and alkyl group or an aryl group; R3 indicates H, an alkyl, an aryl, an acyl, an alkoxycarbonyl or an aryloxycarbonyl group; and the alkyl, aryl, acyl, alkoxycarbonyl and/or aryloxycarbonyl group of the radicals R1, R3, R4 and R5, independently of one another, are substituted or unsubstituted.

摘要翻译： 本发明涉及一种将生物分子固定在载体材料的聚合物表面上的方法，以及根据通式（Ⅳ）和（Ⅴ）可用根据该方法制备的用生物分子官能化的载体材料，其中P表示 载体材料的聚合物表面，M表示通过氨基（式IV）或羰基（式V）与连接体化合物结合的生物分子，R2表示OR4或NR4R5; R1，R4和R5彼此独立地表示H，烷基或芳基; R3表示H，烷基，芳基，酰基，烷氧基羰基或芳氧基羰基; 基团R 1，R 3，R 4和R 5的烷基，芳基，酰基，烷氧基羰基和/或芳氧基羰基彼此独立地是取代或未取代的。

公开(公告)号：US06680204B1

公开(公告)日：2004-01-20

申请号：US09926368

申请日：2002-04-22

申请人： Anthony P. F. Turner ,  David C. Cullen ,  Sergiy A. Piletsky ,  Olena V. Piletska ,  Uwe Schedler ,  David Weston

发明人： Anthony P. F. Turner ,  David C. Cullen ,  Sergiy A. Piletsky ,  Olena V. Piletska ,  Uwe Schedler ,  David Weston

IPC分类号： G01N33543

CPC分类号： G01N33/54353 ,  G01N33/54393 ,  Y10T436/173845

摘要： Reaction of a dialdehyde, particularly phthaldialdehyde (I), with R—Z where Z is a nucleophilic group (preferably SH) and R is polymerisable (e.g. allyl) gives a reactive thioacetal (V) which can react with an amine ligand L—NH2 to produce an isoindole (IV) which may be fluorescent. At some stage, generally before interaction with L—NH2, the R groups are polymerised, possibly leading to self-assembly of the polymer on a metal or SH-bearing surface. Such a coated surface is useful as a transducer in assays or as a binding medium e.g. for chromatography.

摘要翻译： 二醛，特别是二氯二醛（I）与Z是亲核基团（优选SH）和R是可聚合的（例如烯丙基）的RZ反应得到可与胺配体L-NH 2反应的反应性硫代缩醛（V） 可以是荧光的异吲哚（IV）。 在一些阶段，通常在与L-NH 2相互作用之前，R基团聚合，可能导致聚合物在金属或SH载体表面上的自组装。 这样的涂覆表面可用作测定中的换能器或作为结合介质例如 用于色谱法。

公开(公告)号：US07423117B2

公开(公告)日：2008-09-09

申请号：US10451654

申请日：2001-12-03

申请人： Pablo Wessig ,  Jürgen Bendig ,  Uwe Schedler

发明人： Pablo Wessig ,  Jürgen Bendig ,  Uwe Schedler

IPC分类号： C07K1/04 ,  C07K1/06 ,  C07K1/10 ,  C12K17/06 ,  C12N11/06 ,  G01N33/547

CPC分类号： C08J7/12 ,  C07K1/042 ,  Y10S530/816

摘要： The invention concerns a novel surface-functionalized carrier material with a polymeric surface and at least one linker compound according to the general formula (I), which is covalently bound to the surface. In the formula, P indicates the polymeric surface; R2 has the meaning OR4 or NR4R5 and R1, R4 and R5, independently of one another, indicate H, an alkyl group or an aryl group; R3 indicates H, an alkyl, an aryl, an acyl, an alkoxycarbonyl or an aryloxycarbonyl group; and the alkyl, aryl, acyl, alkoxycarbonyl and/or aryloxycarbonyl group of the radicals R1, R3, R4 and R5, independently of one another, are substituted or unsubstituted. The material according to the invention can be very easily produced by photochemical coupling and serves for the solid-phase synthesis of amino acids, peptides, proteins or molecules with at least one peptidic structural unit.

摘要翻译： 本发明涉及具有聚合物表面的新型表面官能化载体材料和根据通式（I）的至少一种连接体化合物，其与该表面共价结合。 在配方中，P表示聚合物表面; R 2具有含义OR 4或NR 4 R 5和R 1， R 4，R 5和R 5彼此独立地表示H，烷基或芳基; R 3表示H，烷基，芳基，酰基，烷氧基羰基或芳氧基羰基; 和R 1，R 3，R 4和R 4的烷基，芳基，酰基，烷氧基羰基和/或芳氧基羰基 > 5个彼此独立地是取代或未取代的。 根据本发明的材料可以非常容易地通过光化学偶联产生，并用于具有至少一个肽结构单元的氨基酸，肽，蛋白质或分子的固相合成。

公开(公告)号：US20090068757A1

公开(公告)日：2009-03-12

申请号：US12279722

申请日：2007-02-16

申请人： Werner Lehmann ,  Uwe Schedler

发明人： Werner Lehmann ,  Uwe Schedler

IPC分类号： G01N21/64 ,  B01J19/00 ,  G01N27/00 ,  G01J3/28 ,  G01N23/04 ,  G01N21/21 ,  B05D1/32 ,  G01N21/00

CPC分类号： B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00427 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725

摘要： The invention relates to an apparatus (100) for detecting analytes (26) in a sample comprising—a base carrier (10); —a multitude of sensor carriers (18) which are arranged on the base carrier (10) and can be assigned to at least two different sensor carrier populations (181, 182, 183); —the sensor carrier populations (181, 182, 183) being defined at least by different sensor molecules (24) which are assigned to the sensor carrier (18) and each have at least one measurable specificity for an analyte (26) or an analyte group, such that the population (181, 182, 183) of the sensor carriers (18) constitutes a coding which enables the assignment of sensor molecules (24) and/or analyte (26). The apparatus is characterized in that the sensor carriers (18) are present without contact to one another with a predetermined mean distance between one another and with a random statistical distribution on the base carrier (10) with regard to the population (181, 182, 183), as a result of which the detection of only a single entity of a sensor carrier (18) in each case is ensured during the analysis of the sensor molecules and/or of the binding analytes.

摘要翻译： 本发明涉及一种用于检测样品中的分析物（26）的装置（100），包括： - 基座（10）; - 多个传感器载体（18），其布置在所述基底载体（10）上并且可分配给至少两个不同的传感器载体群（181,182,183）; 传感器载体群体（181,182,183）至少由分配给传感器载体（18）的不同传感器分子（24）限定，并且每个传感器载体群体对分析物（26）或分析物（26）具有至少一个可测量的特异性 使得传感器载体（18）的群体（181,182,183）构成能够分配传感器分子（24）和/或分析物（26）的编码。 该装置的特征在于，传感器载体（18）不存在彼此的接触，彼此之间具有预定的平均距离，并且与基座（10）相对于群体（181,182， 183），其结果是在传感器分子和/或结合分析物的分析期间，确保在每种情况下只检测传感器载体（18）的单个实体。

公开(公告)号：US06670427B1

公开(公告)日：2003-12-30

申请号：US09762357

申请日：2001-03-26

申请人： Mathias Ulbricht ,  Sergiy Piletski ,  Uwe Schedler ,  Heike Matuschewski

发明人： Mathias Ulbricht ,  Sergiy Piletski ,  Uwe Schedler ,  Heike Matuschewski

IPC分类号： C08G6348

CPC分类号： B01J20/26 ,  B01J20/265 ,  B01J20/267 ,  B01J20/268 ,  B01J20/28016 ,  B01J20/28021 ,  B01J20/28023 ,  B01J20/28033 ,  B01J20/28078

摘要： Template-textured materials in the form of template-textured polymers (TGP) on various surfaces including membranes (i.e, template-textured membranes). Such materials are created by modifying the surface of solid carriers, which, by cross-linking polymerization of functional monomers initiated on the surface of said solid carriers in the presence of a template, leads to stable template prints that subsequently bind template molecules or template derivatives in a specific manner. The invention also relates to methods for the production of TGPs and to the use thereof for substance-specific separation of materials.

摘要翻译： 在包括膜（即模板纹理的膜）的各种表面上的模板织构化聚合物（TGP）形式的模板织构材料。 这样的材料是通过改变固体载体的表面产生的，其通过在模板存在下在所述固体载体的表面上引发的官能单体的交联聚合，导致稳定的模板印迹，随后结合模板分子或模板衍生物 以具体方式。 本发明还涉及生产TGP的方法及其用于物质特异性分离材料的用途。

公开(公告)号：US20050003501A1

公开(公告)日：2005-01-06

申请号：US10451654

申请日：2001-12-03

申请人： Pablo Wessig ,  Jurgen Bendig ,  Uwe Schedler

发明人： Pablo Wessig ,  Jurgen Bendig ,  Uwe Schedler

IPC分类号： C07K1/04 ,  C08J7/12 ,  C07C1/00 ,  C07C2/00 ,  C07C4/00 ,  C07C5/00

CPC分类号： C08J7/12 ,  C07K1/042 ,  Y10S530/816

摘要： The invention concerns a novel surface-functionalized carrier material with a polymeric surface and at least one linker compound according to the general formula (I), which is covalently bound to the surface. In the formula, P indicates the polymeric surface; R2 has the meaning OR4 or NR4R5 and R1, R4 and R5, independently of one another, indicate H, an alkyl group or an aryl group; R3 indicates H, an alkyl, an aryl, an acyl, an alkoxycarbonyl or an aryloxycarbonyl group; and the alkyl, aryl, acyl, alkoxycarbonyl and/or aryloxycarbonyl group of the radicals R1, R3, R4 and R5, independently of one another, are substituted or unsubstituted. The material according to the invention can be very easily produced by photochemical coupling and serves for the solid-phase synthesis of amino acids, peptides, proteins or molecules with at least one peptidic structural unit.

摘要翻译： 本发明涉及具有聚合物表面的新型表面官能化载体材料和根据通式（I）的至少一种连接体化合物，其与该表面共价结合。 在配方中，P表示聚合物表面; R 2具有意义OR 4或NR 4 R 5和R 1，R 4和R 5彼此独立地表示H，烷基或芳基 组; R 3表示H，烷基，芳基，酰基，烷氧基羰基或芳氧基羰基; 基团R 1，R 3，R 4和R 5的烷基，芳基，酰基，烷氧基羰基和/或芳氧基羰基彼此独立地是取代或未取代的。 根据本发明的材料可以非常容易地通过光化学偶联产生，并用于具有至少一个肽结构单元的氨基酸，肽，蛋白质或分子的固相合成。

公开(公告)号：US06753396B2

公开(公告)日：2004-06-22

申请号：US10148819

申请日：2002-09-03

申请人： Mathias Ulbricht ,  Tatinna Sergeyeva ,  Heike Matuschewski ,  Uwe Schedler ,  Sergey A. Piletsky

发明人： Mathias Ulbricht ,  Tatinna Sergeyeva ,  Heike Matuschewski ,  Uwe Schedler ,  Sergey A. Piletsky

IPC分类号： C08F22046

CPC分类号： G01N30/482 ,  B01J20/268 ,  B01J20/281 ,  B01J20/3057 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3276 ,  B01J20/3278 ,  B01J20/3282 ,  B01J20/3285 ,  B01J2220/54

摘要： The invention relates to a method for the production of a template-textured material by synthesis of a template-textured polymer (TTP) by performing crosslinking polymerization of functional monomers on a support in the presence of a template, which method is characterized in that a support having a thin polymer layer on the surface thereof is added with a reaction mixture consisting of polymerization initiator, template, functional monomer, crosslinker, solvent and/or buffer and, following sorption of the reaction mixture in the thin polymer layer, the polymerization is initiated and continued until the absorption capacity of the thin polymer layer for the template-textured polymer (TTP) is reached, and the template is optionally removed in a final step, the support used being selected in such a way that it cannot absorb the reaction solution. The materials of the invention are remarkable for their high binding specificity and selectivity for the template.

摘要翻译： 本发明涉及一种通过在模板存在下通过在载体上进行官能单体的交联聚合来通过合成模板织构化聚合物（TTP）来生产模板织构材料的方法，该方法的特征在于， 在其表面上具有薄聚合物层的载体中加入由聚合引发剂，模板，官能单体，交联剂，溶剂和/或缓冲剂组成的反应混合物，并且在聚合物层中吸附反应混合物后，聚合反应 引发并持续直到达到模板 - 织构聚合物（TTP）的薄聚合物层的吸收能力，并且在最终步骤中任选地除去模板，使用的载体以不能吸收反应的方式进行选择 解。 本发明的材料对于模板的高结合特异性和选择性是显着的。