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    Method for producing template-textured materials with high binding specificity and selectivity and utilization of said materials
    3.
    发明授权
    Method for producing template-textured materials with high binding specificity and selectivity and utilization of said materials 失效
    用于生产具有高结合特异性和所述材料的选择性和利用率的模板织构材料的方法

    公开(公告)号：US06753396B2

    公开(公告)日：2004-06-22

    申请号：US10148819

    申请日：2002-09-03

    申请人： Mathias Ulbricht ,  Tatinna Sergeyeva ,  Heike Matuschewski ,  Uwe Schedler ,  Sergey A. Piletsky

    发明人： Mathias Ulbricht ,  Tatinna Sergeyeva ,  Heike Matuschewski ,  Uwe Schedler ,  Sergey A. Piletsky

    IPC分类号： C08F22046

    CPC分类号： G01N30/482 ,  B01J20/268 ,  B01J20/281 ,  B01J20/3057 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3276 ,  B01J20/3278 ,  B01J20/3282 ,  B01J20/3285 ,  B01J2220/54

    摘要： The invention relates to a method for the production of a template-textured material by synthesis of a template-textured polymer (TTP) by performing crosslinking polymerization of functional monomers on a support in the presence of a template, which method is characterized in that a support having a thin polymer layer on the surface thereof is added with a reaction mixture consisting of polymerization initiator, template, functional monomer, crosslinker, solvent and/or buffer and, following sorption of the reaction mixture in the thin polymer layer, the polymerization is initiated and continued until the absorption capacity of the thin polymer layer for the template-textured polymer (TTP) is reached, and the template is optionally removed in a final step, the support used being selected in such a way that it cannot absorb the reaction solution. The materials of the invention are remarkable for their high binding specificity and selectivity for the template.

    摘要翻译： 本发明涉及一种通过在模板存在下通过在载体上进行官能单体的交联聚合来通过合成模板织构化聚合物（TTP）来生产模板织构材料的方法，该方法的特征在于， 在其表面上具有薄聚合物层的载体中加入由聚合引发剂，模板，官能单体，交联剂，溶剂和/或缓冲剂组成的反应混合物，并且在聚合物层中吸附反应混合物后，聚合反应 引发并持续直到达到模板 - 织构聚合物（TTP）的薄聚合物层的吸收能力，并且在最终步骤中任选地除去模板，使用的载体以不能吸收反应的方式进行选择 解。 本发明的材料对于模板的高结合特异性和选择性是显着的。

    Method for the parallel and combinatory synthesis of compounds bound to a continuous polymeric solid phase supporting material
    4.
    发明授权
    Method for the parallel and combinatory synthesis of compounds bound to a continuous polymeric solid phase supporting material 有权
    平行和组合合成连接聚合物固相支撑材料的化合物的方法

    公开(公告)号：US06515039B1

    公开(公告)日：2003-02-04

    申请号：US09530457

    申请日：2000-06-15

    申请人： Mathias Ulbricht ,  Rudolf Volkmer-Engert ,  Lothar Germeroth ,  Holger Wenschuh

    发明人： Mathias Ulbricht ,  Rudolf Volkmer-Engert ,  Lothar Germeroth ,  Holger Wenschuh

    IPC分类号： C08F246

    CPC分类号： C07K1/042 ,  B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00722 ,  C08F255/02 ,  C08F259/08 ,  C08F291/18 ,  C40B40/14

    摘要： The invention relates to a method for the parallel and combinatory synthesis of compounds bound to a continuous polymeric solid phase supporting material. According to the method, a continuous polymeric solid phase supporting material is prepared, the material comprising a matrix of a supporting polymeric material and graft copolymer chains covalently bound to the supporting matrix, the graft copolymer chains having reactive groups being able to react with organic compounds, thereby forming spatially defined reaction sites. The method then comprises the sequential spotting of synthesis building blocks or reagents at the various reaction sites on the continuous solid phase supporting material to yield a substrate library of compounds bound to the solid phase supporting material, whereby each reaction site on the continuous solid phase supporting material determines the composition of the synthesized compound, which is bound directly on the supporting matrix via the reactive groups of the graft polymer chain.

    摘要翻译： 本发明涉及平行和组合合成结合到连续聚合物固相支撑材料上的化合物的方法。 根据该方法，制备连续的聚合物固相支撑材料，该材料包含支撑聚合物材料的基体和共价结合到支撑基质上的接枝共聚物链，具有反应性基团的接枝共聚物链能够与有机化合物反应 ，从而形成空间定义的反应位点。 该方法然后包括在连续固相支持材料上的各个反应位点上的合成结构单元或试剂的顺序点样，以产生与固相支持材料结合的化合物的底物文库，由此连续固相支持的每个反应位点 材料决定了合成化合物的组成，其通过接枝聚合物链的反应性基团直接结合在载体上。

    Method for isolating nucleic acids from a liquid sample containing nucleic acids
    6.
    发明授权
    Method for isolating nucleic acids from a liquid sample containing nucleic acids 失效
    从含有核酸的液体样品中分离核酸的方法

    公开(公告)号：US06545144B2

    公开(公告)日：2003-04-08

    申请号：US10066509

    申请日：2002-01-30

    申请人： Thomas Kolzau ,  Wilhelm Plüster ,  Mathias Ulbricht

    发明人： Thomas Kolzau ,  Wilhelm Plüster ,  Mathias Ulbricht

    IPC分类号： C07H2100

    CPC分类号： C12Q1/6806 ,  C12N15/1017

    摘要： A method for the isolation of nucleic acids from a liquid sample, which contains nucleic acids, using a filtering device, which has pores, through which the sample is allowed to flow in the course of the isolation, the nucleic acids, and contained in the sample, being retained selectively in or at the pores, wherein the sample is mixed with a concentration of the precipitating agent for nucleic acids, which is sufficient to condense the nucleic acids and the sample is then allowed to flow through the filtering device, the pores of the filtering device used having inner wall regions with variable surface structures, which are constructed differently depending on the medium passed through the pores and by which the permeability of the pores can be adjusted between a state, in which the condensed nucleic acid molecules are retained, and a state, in which the dissolved nucleic acid molecules can pass through.

    摘要翻译： 使用过滤装置从包含核酸的液体样品中分离核酸的方法，所述过滤装置具有孔，允许样品在分离过程中流过其中，并且包含在 样品被选择性地保留在孔中或孔中，其中样品与用于核酸的沉淀剂的浓度混合，其足以冷凝核酸，然后允许样品流过过滤装置，孔 所使用的过滤装置具有可变表面结构的内壁区域，其根据通过孔的介质而不同地构造，并且可以在冷凝的核酸分子保留的状态之间调节孔的渗透性 ，以及溶解的核酸分子能够通过的状态。

    Biocompatible materials
    9.
    发明申请
    Biocompatible materials 审中-公开
    生物相容性材料

    公开(公告)号：US20050053642A1

    公开(公告)日：2005-03-10

    申请号：US10362677

    申请日：2001-08-23

    申请人： Mathias Ulbricht ,  Volkmar Thom ,  Katja Jankova ,  George Altankov ,  Gunnar Jonsson

    发明人： Mathias Ulbricht ,  Volkmar Thom ,  Katja Jankova ,  George Altankov ,  Gunnar Jonsson

    IPC分类号： A61L27/34 ,  A61L27/50 ,  A61L33/00

    CPC分类号： B82Y30/00 ,  A61L27/34 ,  A61L27/50 ,  B82Y5/00

    摘要： The present invention teaches a novel approach of creating biocmpatible surfaces, said surfaces being capable of functionally interact with biological material. SAid biocompatible surfaces comrise at least two comonents, such as a hydrophobic substratum and a macromolecule of hydrophilic nature, which, in a cooperativity, form together the novel biocoompatible surfaces. The novel approach is ased on contacting said hydrophobic substratum with a laterally patterned monomolecular layer of said hydrophilic and flexible macromolecules, exhibiting a pronounced excluded volume. The htus formed two component surface is, in respect to polarity and morphology, a molecularly heterogeneous surface. Structural features of said macromolecular monolayer (as e.g. the layer thickness or its lateral density) are determined by: i) the structural features of the layer forming macromolecules (as e.g. their MW or their molecular architecture) and ii) the method of creating said monomolecular layer (as e.g. by physi- or chemisorbing, or by chemically binding said macromolecules). The structural features of the layer forming macromolecules(s) is in turn determined by synthesis. AMount and conformation and thus also biological activity of biological material (as e.g. polypeptides) which contact the novel biocompatible surface, is determined and maintained by the cooperative action of the underlying hydrophobic substratum and the macromolecular layer. In this way it becomes possible to maintain and control biological interactions between said contacted polypeptides and other biological compounds as e.g. cells, antibodies and the like. Consequently, the present invention aims to reduce and/or eliminate the deactivation and/or denaturation associated with the contacting of polypeptides and/or other biological material to a hydrophobic substratum surface.

    摘要翻译： 本发明教导了一种创建生物相容性表面的新方法，所述表面能够与生物材料进行功能相互作用。 所述生物相容性表面至少包含两种共聚物，例如疏水性底物和具有亲水性质的大分子，其以协同作用形成新的生物相容性表面。 该新方法是将所述疏水性底物与所述亲水和柔性大分子的横向图案化单分子层接触，表现出显着排除的体积。 形成双组分表面的htus在极性和形态方面是分子异质表面。 所述大分子单层的结构特征（例如层厚度或其侧向密度）通过以下方式确定：i）形成大分子的层的结构特征（例如它们的MW或其分子结构）和ii）产生所述单分子的方法 层（例如通过物理或化学吸附，或通过化学结合所述大分子）。 层的形成大分子的结构特征又由合成决定。 通过潜在的疏水性底物和大分子层的协同作用确定并保持了与新生物相容性表面接触的生物材料（如多肽）的生物活性和生物学活性。 以这种方式，可以维持和控制所述接触的多肽和其他生物化合物之间的生物相互作用，例如。 细胞，抗体等。 因此，本发明旨在减少和/或消除与多肽和/或其他生物材料与疏水底物表面的接触相关的失活和/或变性。

    Template-textured materials, methods for the production and use thereof
    10.
    发明授权
    Template-textured materials, methods for the production and use thereof 失效
    模板纹理材料，生产和使用方法

    公开(公告)号：US06670427B1

    公开(公告)日：2003-12-30

    申请号：US09762357

    申请日：2001-03-26

    申请人： Mathias Ulbricht ,  Sergiy Piletski ,  Uwe Schedler ,  Heike Matuschewski

    发明人： Mathias Ulbricht ,  Sergiy Piletski ,  Uwe Schedler ,  Heike Matuschewski

    IPC分类号： C08G6348

    CPC分类号： B01J20/26 ,  B01J20/265 ,  B01J20/267 ,  B01J20/268 ,  B01J20/28016 ,  B01J20/28021 ,  B01J20/28023 ,  B01J20/28033 ,  B01J20/28078

    摘要： Template-textured materials in the form of template-textured polymers (TGP) on various surfaces including membranes (i.e, template-textured membranes). Such materials are created by modifying the surface of solid carriers, which, by cross-linking polymerization of functional monomers initiated on the surface of said solid carriers in the presence of a template, leads to stable template prints that subsequently bind template molecules or template derivatives in a specific manner. The invention also relates to methods for the production of TGPs and to the use thereof for substance-specific separation of materials.

    摘要翻译： 在包括膜（即模板纹理的膜）的各种表面上的模板织构化聚合物（TGP）形式的模板织构材料。 这样的材料是通过改变固体载体的表面产生的，其通过在模板存在下在所述固体载体的表面上引发的官能单体的交联聚合，导致稳定的模板印迹，随后结合模板分子或模板衍生物 以具体方式。 本发明还涉及生产TGP的方法及其用于物质特异性分离材料的用途。