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    Microfluidic device and array disk
    2.
    发明授权
    Microfluidic device and array disk 有权

    公开(公告)号：US10022718B2

    公开(公告)日：2018-07-17

    申请号：US14922696

    申请日：2015-10-26

    Applicant: Arizona Board of Regents on behalf of Arizona State University

    Inventor： Rhett Martineau ,  Jeff Houkal ,  Shih-Hui Chao ,  Weimin Gao ,  Shufang Ci ,  Deirdre Meldrum

    IPC: B01L3/00 ,  B01L7/00

    Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.

    Microfluidic device and array disk
    3.
    发明授权
    Microfluidic device and array disk 有权

    公开(公告)号：US10471426B2

    公开(公告)日：2019-11-12

    申请号：US16035464

    申请日：2018-07-13

    Applicant: Arizona Board of Regents on behalf of Arizona State University

    Inventor： Rhett Martineau ,  Jeff Houkal ,  Shih-Hui Chao ,  Weimin Gao ,  Shufang Ci ,  Deirdre Meldrum

    IPC: B01L3/00 ,  G01N35/00 ,  G01N1/10 ,  B01L7/00

    Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.

    Methods for digital readout quantification of nucleic acids
    6.
    发明授权
    Methods for digital readout quantification of nucleic acids 有权

    公开(公告)号：US11345954B2

    公开(公告)日：2022-05-31

    申请号：US16323393

    申请日：2017-08-07

    Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

    Inventor： Rhett Martineau ,  Deirdre Meldrum

    IPC: C12Q1/6851 ,  C12Q1/6853

    Abstract: The invention provides methods for detecting specific nucleic acids using a loop-mediated isothermal amplification (LAMP) reaction and providing a digital readout of the results. One method teaches separating target specificity from indicator detection in a two-stage multiple-threshold array (MTA). During the first stage amplicons incorporating both target sequence and an indicator sequence are synthesized. During the second stage of the reaction, the indicator sequence is targeted and amplified to produce visual results that may be digitalized. Another method teaches a competitive MTA, in which a reference sequence is used to compete against target sequence during amplification producing threshold responses, thus enabling digital readout. Using either method, quantitation is achieved without the need for continuous monitoring of the reaction and the end-point readout is amenable to visual inspection. Methods of the invention are especially useful in circumstances where there is lower or intermittent power supply.

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