公开(公告)号：US10471426B2

公开(公告)日：2019-11-12

申请号：US16035464

申请日：2018-07-13

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Rhett Martineau ,  Jeff Houkal ,  Shih-Hui Chao ,  Weimin Gao ,  Shufang Ci ,  Deirdre Meldrum

IPC: B01L3/00 ,  G01N35/00 ,  G01N1/10 ,  B01L7/00

Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.

公开(公告)号：US20160114323A1

公开(公告)日：2016-04-28

申请号：US14922696

申请日：2015-10-26

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Rhett Martineau ,  Jeff Houkal ,  Shih-Hui Chao ,  Weimin Gao ,  Shufang Ci ,  Deirdre Meldrum

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0803 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/161 ,  B01L2400/049 ,  B01L2400/0683

Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.

Abstract translation: 本发明涉及用于“一锅”分离的化学反应的微流体装置和阵列盘。 阵列盘包括多个扇区，其中每个扇区包括一个微流体装置。 微流体装置包括流体输送通道和孔阵列，其中流体输送通道以蛇形结构输送到孔中。 在一些实施例中，流体输送通道直接在孔阵列的上方。 在其他实施例中，流体输送通道偏离孔阵列，使得从流体输送通道分支的侧通道将流体输送到孔中。 微流体装置的井包括形成地板，井或井的地板或墙壁的至少一部分的透气膜。 在优选实施例中，井是圆柱形的。

公开(公告)号：US20180318835A1

公开(公告)日：2018-11-08

申请号：US16035464

申请日：2018-07-13

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Rhett Martineau ,  Jeff Houkal ,  Shih-Hui Chao ,  Weimin Gao ,  Shufang Ci ,  Deirdre Meldrum

IPC: B01L3/00 ,  B01L7/00

CPC classification number: B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0803 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/161 ,  B01L2400/049 ,  B01L2400/0683

Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.

公开(公告)号：US10221443B2

公开(公告)日：2019-03-05

申请号：US15135150

申请日：2016-04-21

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Deirdre Meldrum ,  Shih-Hui (Joseph) Chao ,  Thai Tran ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Andrew Hatch ,  Weimin Gao ,  David Richardson

IPC: C12Q1/68 ,  C12Q1/6806 ,  C12M1/00 ,  G01N1/28 ,  C12Q1/6848 ,  B01L3/02 ,  B01L3/00 ,  C12Q1/6841 ,  C12Q1/686

Abstract: Systems and methods for in situ laser lysis for analysis of biological tissue (live, fixed, frozen or otherwise preserved) at single cell resolution in 3D. For example, a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing at least one target molecule is provided. The system collects cellular contents, performs highly multiplexed (RT-qPCR or RNA-seq), and sequentially (cell-by-cell) reconstructs a 3D spatial map of mRNA expression of the tissue with a large number of genes. A 3D spatial map of the DNA, RNA, and/or proteins can be generated for each cell in the tissue.

公开(公告)号：US10022718B2

公开(公告)日：2018-07-17

申请号：US14922696

申请日：2015-10-26

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Rhett Martineau ,  Jeff Houkal ,  Shih-Hui Chao ,  Weimin Gao ,  Shufang Ci ,  Deirdre Meldrum

IPC: B01L3/00 ,  B01L7/00

Abstract: The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.

公开(公告)号：US20200063197A1

公开(公告)日：2020-02-27

申请号：US16611799

申请日：2018-05-15

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Deirdre Meldrum ,  Shufang Ci ,  Shih-Hui (Joseph) Chao ,  Rhett Martineau ,  Weimin Gao

IPC: C12Q1/6851 ,  G16B40/10 ,  C12Q1/70 ,  G01N21/78 ,  G01N21/27 ,  G06T7/90 ,  G06T7/00

Abstract: The present disclosure relates to real-time quantification using loop-mediated isothermal amplification, in particular real-time colorimetric reverse transcription quantitative loop-mediated isothermal amplification (RT-qLAMP). In some embodiments, RT-qLAMP is used to diagnose the presence of and also quantitate the amount of Zika virus in a sample.

公开(公告)号：US20160237476A1

公开(公告)日：2016-08-18

申请号：US15135150

申请日：2016-04-21

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Deirdre Meldrum ,  Shih-Hui (Joseph) Chao ,  Thai Tran ,  Laimonas Kelbauskas ,  Jeff Houkal ,  Andrew Hatch ,  Weimin Gao ,  David Richardson

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/0293 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0864 ,  C12M47/06 ,  C12Q1/6841 ,  C12Q1/6848 ,  C12Q1/686 ,  G01N1/286 ,  G01N2001/2886 ,  C12Q2547/101

Abstract: Systems and methods for in situ laser lysis for analysis of biological tissue (live, fixed, frozen or otherwise preserved) at single cell resolution in 3D. For example, a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing at least one target molecule is provided. The system collects cellular contents, performs highly multiplexed (RT-qPCR or RNA-seq), and sequentially (cell-by-cell) reconstructs a 3D spatial map of mRNA expression of the tissue with a large number of genes. A 3D spatial map of the DNA, RNA, and/or proteins can be generated for each cell in the tissue.

Abstract translation: 用于原位激光裂解的系统和方法，用于分析3D中单细胞分辨率的生物组织（活，固定，冷冻或以其他方式保存）。 例如，用于原位裂解单个细胞的系统和方法，包括以下步骤：捕获包含细胞内容物的组织样品，使组织样品经受连续流体流的流动，用激光裂解组织样品的选定区域 从而从组织样品释放至少一部分细胞含量，从流中的细胞含量回收至少一种靶分子，并且提供至少一种靶分子的加工。 系统收集细胞内容，进行高度多重化（RT-qPCR或RNA-seq），并依次（逐个细胞）重构具有大量基因的组织的mRNA表达的3D空间图。 可以为组织中的每个细胞产生DNA，RNA和/或蛋白质的3D空间图。