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公开(公告)号：US20140357742A1
公开(公告)日：2014-12-04
申请号：US14363919
申请日：2012-12-12
Applicant: ASAHI KASEI CHEMICALS CORPORATION
Inventor： Naoyuki Shinohara , Yuta Sato
CPC classification number: B01J20/265 , B01J20/28023 , B01J20/288 , B01J20/3078 , B01J20/321 , B01J20/3217 , B01J20/327 , B01J20/3278 , B01J20/3293 , B01J39/07 , B01J39/20 , B01J39/26 , B01J41/07 , B01J41/13 , B01J41/14 , B01J41/20 , B01J47/12 , B01J47/127 , B01J47/133 , B01J2220/52 , C07K1/22 , C08J5/2243 , C08J2351/06 , C08J2351/08
Abstract: A method for producing a protein adsorbent comprising a substrate and a molecular chain fixed on the surface of the substrate is disclosed. The method comprises, in this order: a dry-heat treatment step of heating a pretreatment adsorbent comprising the substrate and the molecular chain fixed on the surface of the substrate, in which the molecular chain contains a weak electrolytic ion-exchange group; and a wet-heat treatment step of heating the pretreatment adsorbent in a moistened state with a liquid or steam to obtain the protein adsorbent.
Abstract translation: 公开了一种生产蛋白质吸附剂的方法，该方法包括固定在基底表面上的基底和分子链。 该方法按照以下顺序进行：将包含基板的预处理吸附剂和固定在基板表面上的分子链进行加热的干热处理工序，其中分子链含有弱电解离子交换基团; 以及湿润处理步骤，用液体或蒸汽将预处理吸附剂加湿，得到蛋白质吸附剂。
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公开(公告)号：US20140235837A1
公开(公告)日：2014-08-21
申请号：US14347903
申请日：2012-09-28
Applicant: ASAHI KASEI CHEMICALS CORPORATION
Inventor： Yuta Sato , Naoyuki Shinohara , Hironobu Shirataki
IPC: C07K1/14
CPC classification number: C07K1/145 , B01J39/07 , B01J41/07 , B01J47/12 , C07K1/14 , C07K1/16 , C07K1/18 , C07K1/22
Abstract: It is an object of the present invention to provide a method for eluting an adsorbed protein that suppresses a decrease in protein adsorption ability, in a method for purifying a protein using a protein-adsorbing porous membrane.The present invention provides a method for purifying a protein, comprising an adsorption step and an elution step, wherein in the elution step, at least one eluent is passed in the opposite direction with respect to the direction of the passage of a stock solution containing an adsorption target protein, in the adsorption step.
Abstract translation: 本发明的目的是提供一种洗脱蛋白质吸附能力降低的吸附蛋白质的洗脱方法，使用蛋白质吸附多孔膜的蛋白质的纯化方法。 本发明提供一种纯化蛋白质的方法，其包括吸附步骤和洗脱步骤，其中在洗脱步骤中，至少一种洗脱液在相对于含有 吸附目标蛋白，在吸附步骤中。
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公开(公告)号：US09545627B2
公开(公告)日：2017-01-17
申请号：US14363919
申请日：2012-12-12
Applicant: ASAHI KASEI CHEMICALS CORPORATION
Inventor： Naoyuki Shinohara , Yuta Sato
IPC: B01J41/20 , B01J39/04 , B01J39/20 , B01J39/26 , B01J41/04 , B01J41/12 , B01J41/14 , B01J47/12 , B01J20/30 , B01J20/32 , C07K1/22
CPC classification number: B01J20/265 , B01J20/28023 , B01J20/288 , B01J20/3078 , B01J20/321 , B01J20/3217 , B01J20/327 , B01J20/3278 , B01J20/3293 , B01J39/07 , B01J39/20 , B01J39/26 , B01J41/07 , B01J41/13 , B01J41/14 , B01J41/20 , B01J47/12 , B01J47/127 , B01J47/133 , B01J2220/52 , C07K1/22 , C08J5/2243 , C08J2351/06 , C08J2351/08
Abstract: A method for producing a protein adsorbent comprising a substrate and a molecular chain fixed on the surface of the substrate is disclosed. The method comprises, in this order: a dry-heat treatment step of heating a pretreatment adsorbent comprising the substrate and the molecular chain fixed on the surface of the substrate, in which the molecular chain contains a weak electrolytic ion-exchange group; and a wet-heat treatment step of heating the pretreatment adsorbent in a moistened state with a liquid or steam to obtain the protein adsorbent.
Abstract translation: 公开了一种生产蛋白质吸附剂的方法，该方法包括固定在基底表面上的基底和分子链。 该方法按照以下顺序进行：将包含基板的预处理吸附剂和固定在基板表面上的分子链进行加热的干热处理工序，其中分子链含有弱电解离子交换基团; 以及湿润处理步骤，用液体或蒸汽将预处理吸附剂加湿，得到蛋白质吸附剂。
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公开(公告)号：US09428544B2
公开(公告)日：2016-08-30
申请号：US14347903
申请日：2012-09-28
Applicant: ASAHI KASEI CHEMICALS CORPORATION
Inventor： Yuta Sato , Naoyuki Shinohara , Hironobu Shirataki
CPC classification number: C07K1/145 , B01J39/07 , B01J41/07 , B01J47/12 , C07K1/14 , C07K1/16 , C07K1/18 , C07K1/22
Abstract: It is an object of the present invention to provide a method for eluting an adsorbed protein that suppresses a decrease in protein adsorption ability, in a method for purifying a protein using a protein-adsorbing porous membrane.The present invention provides a method for purifying a protein, comprising an adsorption step and an elution step, wherein in the elution step, at least one eluent is passed in the opposite direction with respect to the direction of the passage of a stock solution containing an adsorption target protein, in the adsorption step.
Abstract translation: 本发明的目的是提供一种洗脱蛋白质吸附能力降低的吸附蛋白质的洗脱方法，使用蛋白质吸附多孔膜的蛋白质的纯化方法。 本发明提供一种纯化蛋白质的方法，其包括吸附步骤和洗脱步骤，其中在洗脱步骤中，至少一种洗脱液在相对于含有 吸附目标蛋白，在吸附步骤中。
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