公开(公告)号：US20230416793A1

公开(公告)日：2023-12-28

申请号：US18307947

申请日：2023-04-27

申请人： Ajinomoto Co., Inc.

发明人： Seiko Hirano ,  Kota Inoue ,  Kazuyuki Hayashi ,  Kosuke Yokota ,  Mika Moriya ,  Tomoko Suzuki ,  Yoshitomo Kadokura ,  Takeshi Nagahiko ,  Akari Tashiro

IPC分类号： C12P13/14 ,  C12N15/77 ,  C12N9/12 ,  C12N9/16 ,  C12N9/02 ,  C12N9/10 ,  C12N9/04 ,  C12N9/88

CPC分类号： C12P13/14 ,  C12N15/77 ,  C12N9/1217 ,  C12N9/16 ,  C12N9/0008 ,  C12N9/1096 ,  C12N9/0006 ,  C12N9/88 ,  C12R2001/15

摘要： Provided is a method of producing an L-amino acid such as L-glutamic acid and the like. An L-amino-acid is produced by cultivating a coryneform bacterium having L-amino acid-producing ability in a culture medium, which has been modified so as to have one or more of the following modifications: (A) a modification for increasing activity of acetate kinase, (B) a modification for increasing activity of fructose-1,6-bisphosphatase, (C) a modification for decreasing activity of pyruvate dehydrogenase, (D) a modification for decreasing activity of aspartate transaminase, and (E) a modification for decreasing activity of malic enzyme; and collecting the L-amino acid from the culture medium and/or the bacterial cells.

公开(公告)号：US10745725B2

公开(公告)日：2020-08-18

申请号：US15937336

申请日：2018-03-27

申请人： AJINOMOTO CO., INC.

发明人： Kosuke Yokota ,  Kazuyuki Hayashi

IPC分类号： C12P13/14 ,  C12P13/24 ,  C12P13/10 ,  C12N9/88 ,  C12N15/52

摘要： A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing a bacterium having an ability to produce an L-amino acid, which has been modified so that the activity of a non-PTS fructose-uptake carrier and the activity of fructokinase are both increased, in a medium containing fructose, and collecting the L-amino acid from the medium or cells of the bacterium.

公开(公告)号：US20130295621A1

公开(公告)日：2013-11-07

申请号：US13914872

申请日：2013-06-11

申请人： AJINOMOTO CO., INC.

发明人： Yousuke Nishio ,  Youko Yamamoto ,  Kazuteru Yamada ,  Kosuke Yokota

IPC分类号： C12P13/14

CPC分类号： C12P13/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12P7/50 ,  C12P7/58 ,  C12P13/001 ,  C12P13/04 ,  C12P13/10 ,  C12P13/24 ,  C12Y101/01113 ,  C12Y102/01026 ,  C12Y103/05001 ,  C12Y301/01068 ,  C12Y402/01082

摘要： A target substance can be produced by culturing a bacterium having an ability to produce 2-ketoglutaric acid or a derivative thereof, and an ability to produce xylonic acid from xylose, which is imparted with xylonate dehydratase activity, 2-keto-3-deoxyxylonate dehydratase activity and 2-ketoglutaric semialdehyde dehydrogenase activity, or in which these activities are enhanced, in a medium containing xylose as a carbon source to produce and accumulate the target substance in the medium, and collecting the target substance from the medium.

摘要翻译： 目标物质可以通过培养具有生产2-酮戊二酸或其衍生物的能力的细菌和从赋予木糖酸脱水酶活性的木糖中产生木糖酸的能力，2-酮-3-脱氧木糖酸脱水酶 活性和2-酮戊二醛半醛脱氢酶活性，或其中这些活性增强的培养基，在含有木糖作为碳源的培养基中培养和累积目标物质，并从培养基中收集目标物质。

公开(公告)号：US20180282773A1

公开(公告)日：2018-10-04

申请号：US15937336

申请日：2018-03-27

申请人： AJINOMOTO CO., INC.

发明人： Kosuke Yokota ,  Kazuyuki Hayashi

IPC分类号： C12P13/14 ,  C12P13/24 ,  C12P13/10 ,  C12N9/88 ,  C12N15/52

摘要： A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing a bacterium having an ability to produce an L-amino acid, which has been modified so that the activity of a non-PTS fructose-uptake carrier and the activity of fructokinase are both increased, in a medium containing fructose, and collecting the L-amino acid from the medium or cells of the bacterium.

公开(公告)号：US09045789B2

公开(公告)日：2015-06-02

申请号：US13914872

申请日：2013-06-11

申请人： AJINOMOTO CO., INC.

发明人： Yousuke Nishio ,  Youko Yamamoto ,  Kazuteru Yamada ,  Kosuke Yokota

IPC分类号： C12P13/14 ,  C12P7/50 ,  C12N15/52 ,  C12N9/04 ,  C12N9/02 ,  C12N9/16 ,  C12N9/88 ,  C12P7/58 ,  C12P13/00 ,  C12P13/04 ,  C12P13/10 ,  C12P13/24

CPC分类号： C12P13/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/16 ,  C12N9/88 ,  C12N15/52 ,  C12P7/50 ,  C12P7/58 ,  C12P13/001 ,  C12P13/04 ,  C12P13/10 ,  C12P13/24 ,  C12Y101/01113 ,  C12Y102/01026 ,  C12Y103/05001 ,  C12Y301/01068 ,  C12Y402/01082

摘要： A target substance can be produced by culturing a bacterium having an ability to produce 2-ketoglutaric acid or a derivative thereof, and an ability to produce xylonic acid from xylose, which is imparted with xylonate dehydratase activity, 2-keto-3-deoxyxylonate dehydratase activity and 2-ketoglutaric semialdehyde dehydrogenase activity, or in which these activities are enhanced, in a medium containing xylose as a carbon source to produce and accumulate the target substance in the medium, and collecting the target substance from the medium.

摘要翻译： 目标物质可以通过培养具有生产2-酮戊二酸或其衍生物的能力的细菌和从赋予木糖酸脱水酶活性的木糖中产生木糖酸的能力，2-酮-3-脱氧木糖酸脱水酶 活性和2-酮戊二醛半醛脱氢酶活性，或其中这些活性增强的培养基，在含有木糖作为碳源的培养基中培养和累积目标物质，并从培养基中收集目标物质。