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公开(公告)号：US09371353B2
公开(公告)日：2016-06-21
申请号：US14491683
申请日：2014-09-19
申请人： Ajinomoto Co., Inc.
IPC分类号： C07H1/02 , C07H21/00 , C07H1/00 , C07H19/06 , C07H19/073 , C07H19/173
CPC分类号： C07H21/00 , C07H1/00 , C07H1/02 , C07H19/06 , C07H19/073 , C07H19/173
摘要： The present invention provides a protected nucleotide for elongation, which can be purified efficiently and in a high yield by a liquid-liquid extraction operation, and can achieve an oligonucleotide production method by a phosphoramidite method.It has been found that the above-mentioned problem can be solved by a particular oligonucleotide comprising a protected base and/or particular oligonucleotide protected by a branched chain-containing aromatic group at 3′-position.
摘要翻译： 本发明提供了用于延伸的被保护的核苷酸，其可以通过液 - 液萃取操作有效地和高产率地纯化，并且可以通过亚磷酰胺方法实现寡核苷酸制备方法。 已经发现，上述问题可以通过包含受保护碱和/或在3'-位具有支链含芳族基团保护的特定寡核苷酸的特定寡核苷酸来解决。
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公开(公告)号：US10919928B2
公开(公告)日：2021-02-16
申请号：US16009533
申请日：2018-06-15
申请人： AJINOMOTO CO., INC.
发明人： Kunihiro Hirai , Satoshi Katayama , Naoko Hirose , Taisuke Ichimaru , Ken Yamashita , Daisuke Takahashi
IPC分类号： C07H21/02 , C07H21/04 , C12N15/09 , C07H19/167 , C07H19/20 , C07H1/00 , C07H19/10 , C07H21/00 , C07H19/073 , C07H19/173 , C07H19/067
摘要： Oligonucleotides may be produced by a process, including (1) condensing a nucleoside, nucleotide or oligonucleotide (b), and a nucleoside, nucleotide or oligonucleotide (a), or a substituted nucleotide or oligonucleotide (α) in a non-polar solvent to give a reaction solution containing a phosphite triester product (c); (3) oxidizing or sulfurizing the phosphite triester product (c) to give a reaction solution containing an oligonucleotide (d) wherein the 5′-hydroxy group is protected; (4) deprotecting the oligonucleotide (d) to give a reaction solution containing an oligonucleotide (e) wherein the 5′-hydroxy group is not protected; and (6) adding a polar solvent to the reaction solution containing the oligonucleotide (e) and purifying the oligonucleotide (e) by solid-liquid separation, wherein said nucleoside, nucleotide or oligonucleotide (a) or said substituted nucleotide or oligonucleotide (α) is a compound represented by formula (a-i): wherein Base, Rp1, R10, m, L, Y, and Z are defined herein.
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公开(公告)号：US08846885B2
公开(公告)日：2014-09-30
申请号：US13768486
申请日：2013-02-15
申请人： Ajinomoto Co., Inc.
IPC分类号： C07H21/02 , C07H21/04 , C07H21/00 , C07H1/00 , C07H19/06 , C07H19/173 , C07H19/073
CPC分类号： C07H21/00 , C07H1/00 , C07H1/02 , C07H19/06 , C07H19/073 , C07H19/173
摘要： The present invention provides a protected nucleotide for elongation, which can be purified efficiently and in a high yield by a liquid-liquid extraction operation, and can achieve an oligonucleotide production method by a phosphoramidite method.It has been found that the above-mentioned problem can be solved by a particular oligonucleotide comprising a protected base and/or particular oligonucleotide protected by a branched chain-containing aromatic group at 3′-position.
摘要翻译： 本发明提供了用于延伸的被保护的核苷酸，其可以通过液 - 液萃取操作有效地和高产率地纯化，并且可以通过亚磷酰胺方法实现寡核苷酸制备方法。 已经发现，上述问题可以通过包含受保护碱和/或在3'-位具有支链含芳族基团保护的特定寡核苷酸的特定寡核苷酸来解决。
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公开(公告)号：US10464966B2
公开(公告)日：2019-11-05
申请号：US15655280
申请日：2017-07-20
申请人： AJINOMOTO CO., INC.
发明人： Kunihiro Hirai , Satoshi Katayama , Naoko Hirose , Ken Yamashita , Taisuke Ichimaru , Daisuke Takahashi
IPC分类号： C07K1/32 , C07H21/02 , C07K1/30 , C07H21/04 , C07H21/00 , C07H23/00 , C07F7/18 , C07C43/10 , C07C43/205 , C07C49/84 , C07C69/157 , C07C69/78 , C07C233/47 , C07D295/108 , C07C69/017 , C07C69/24 , C07C69/757 , C07C69/92 , C07C69/94 , C07C43/23
摘要： Precipitation promoters, which are an organic compound having one or more linear aliphatic hydrocarbon groups having not less than 10 carbon atoms, wherein the aliphatic hydrocarbon group has not less than 20 carbon atoms in total are useful for precipitating an organic compound protected by an organic group having one or more aliphatic hydrocarbon groups having not less than 10 carbon atoms from a solvent.
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公开(公告)号：US20180291056A1
公开(公告)日：2018-10-11
申请号：US16014536
申请日：2018-06-21
申请人： AJINOMOTO CO., INC.
发明人： Ken YAMASHITA , Kunihiro Hirai , Satoshi Katayama , Taisuke Ichimaru , Daisuke Takahashi , Naoko Hirose
IPC分类号： C07H21/04
摘要： The present invention aims to provide a more stable and efficient method for producing oligonucleotide, particularly, oligonucleotide having various functional groups linked to the 3′-terminal and the like. Efficient production of oligonucleotide becomes possible by a production method of an oligonucleotide represented by the formula (Ia-2) (each symbol is as defined in the DESCRIPTION) and having a functional group at the 3′-terminal, the method including a step of subjecting an oligonucleic acid with 3′-terminal protected by a silyl-protecting group to 3′-terminal-selective deprotection under desilylation conditions that do not affect protecting groups other than the silyl group, subjecting same to phosphitylation conditions with a phosphoramidite reagent that do not affect protecting groups on the oligonucleic acid to give a 3′-terminal-phosphoramidited oligonucleotide represented by the formula (Ia-1) (each symbol is as defined in the DESCRIPTION), and linking a functional group to the 3′-terminal of the 3′-terminal phosphoramidited oligonucleotide directly or via a linker, and the like.
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公开(公告)号：US20130267697A1
公开(公告)日：2013-10-10
申请号：US13768486
申请日：2013-02-15
申请人： Ajinomoto Co., Inc.
CPC分类号： C07H21/00 , C07H1/00 , C07H1/02 , C07H19/06 , C07H19/073 , C07H19/173
摘要： The present invention provides a protected nucleotide for elongation, which can be purified efficiently and in a high yield by a liquid-liquid extraction operation, and can achieve an oligonucleotide production method by a phosphoramidite method.It has been found that the above-mentioned problem can be solved by a particular oligonucleotide comprising a protected base and/or particular oligonucleotide protected by a branched chain-containing aromatic group at 3′-position.
摘要翻译： 本发明提供了用于延伸的被保护的核苷酸，其可以通过液 - 液萃取操作有效地和高产率地纯化，并且可以通过亚磷酰胺方法实现寡核苷酸制备方法。 已经发现，上述问题可以通过包含受保护碱和/或在3'-位具有支链含芳族基团保护的特定寡核苷酸的特定寡核苷酸来解决。
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