公开(公告)号：US5236567A

公开(公告)日：1993-08-17

申请号：US528088

申请日：1990-05-24

申请人： Akira Nanba ,  Masahiro Fukaya ,  Hajime Okumura ,  Yoshiya Kawamura

发明人： Akira Nanba ,  Masahiro Fukaya ,  Hajime Okumura ,  Yoshiya Kawamura

IPC分类号： C12Q1/00

CPC分类号： C12Q1/004 ,  Y10S435/817 ,  Y10S436/806

摘要： An enzyme sensor, which comprises an enzyme-modified electrode and a counter electrode, wherein the enzyme-modified electrode comprises, as electrode components, an enzyme and/or an enzyme-containing substance and mediator. The enzyme sensor is useful in analysis, such as the analysis of compounds in foods or components in the living body, the diagnosis of diseases and the control of reaction processes. The preparation of the enzyme-modified electrode is also described.

公开(公告)号：US5914257A

公开(公告)日：1999-06-22

申请号：US987260

申请日：1992-12-07

申请人： Masahiro Fukaya ,  Kenji Tayama ,  Toshimi Tamaki ,  Haruko Izumo ,  Hajime Okumura ,  Yoshiya Kawamura

发明人： Masahiro Fukaya ,  Kenji Tayama ,  Toshimi Tamaki ,  Haruko Izumo ,  Hajime Okumura ,  Yoshiya Kawamura

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N15/09 ,  C12N15/53 ,  C12P7/54 ,  C12R1/02

CPC分类号： C12N9/0008 ,  C12P7/54 ,  C12Y102/01003 ,  C12Y102/01004 ,  C12Y102/01005

摘要： This invention relates to the structural gene for the membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said structural gene having a molecular size of about 3.6 Kb and having a restriction enzyme cleavage map as shown in FIG. 1; to a plasmid containing said structural gene; to an acetic acid bacterium transformed by said plasmid; and to an acetic acid fermentation process using said transformant.

摘要翻译： 本发明涉及来自属于醋杆菌属的微生物的膜结合醛脱氢酶的结构基因，所述结构基因具有约3.6Kb的分子大小，并具有如图1所示的限制酶切割图。 1; 涉及含有所述结构基因的质粒; 向由所述质粒转化的乙酸细菌; 以及使用所述转化体的乙酸发酵方法。

公开(公告)号：US5344777A

公开(公告)日：1994-09-06

申请号：US985458

申请日：1992-12-03

申请人： Toshimi Tamaki ,  Hiroshi Takemura ,  Kenji Tayama ,  Masahiro Fukaya ,  Hajime Okumura ,  Yoshiya Kawamura

发明人： Toshimi Tamaki ,  Hiroshi Takemura ,  Kenji Tayama ,  Masahiro Fukaya ,  Hajime Okumura ,  Yoshiya Kawamura

IPC分类号： C12N9/04 ,  C07K16/40 ,  C12N1/21 ,  C12N15/09 ,  C12N15/53 ,  C12R1/01 ,  C12R1/02 ,  C12N15/31 ,  C12N15/74

CPC分类号： C07K16/40 ,  C12N9/0006 ,  Y10S435/823

摘要： There is provided a structural gene of membrane-bound alcohol dehydrogenase complex having a molecular size of about 7.0 kilo base which is derived from a microorganism belonging to the genus Acetobacter represented by Acetobacter altoacetigenes and shown by the nucleotide sequence of SEQ ID. NO. 1 and SEQ ID NO. 2. This enzyme increases the efficiency of acetic acid fermentation and may be effectively utilized for quantitative determination of alcohol.

摘要翻译： 本发明提供一种具有约7.0千碱基的膜结合醇脱氢酶复合物的结构基因，该结构基因来源于由醋杆菌属（Acetobacter altoacetigenes）代表的属于醋杆菌属的微生物，由SEQ ID NO： 没有。 1和SEQ ID NO： 这种酶提高了乙酸发酵的效率，可以有效地用于定量测定酒精。

公开(公告)号：US5726565A

公开(公告)日：1998-03-10

申请号：US511476

申请日：1995-08-04

申请人： Shunichi Uchiyama ,  Takeshi Sato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Masahiro Fukaya ,  Hiroaki Ebisuya ,  Ko Furukawa ,  Kazuyo Kajino ,  Sumio Akita

发明人： Shunichi Uchiyama ,  Takeshi Sato ,  Hirofumi Akano ,  Yoshiya Kawamura ,  Masahiro Fukaya ,  Hiroaki Ebisuya ,  Ko Furukawa ,  Kazuyo Kajino ,  Sumio Akita

IPC分类号： G01N27/42 ,  G01N33/18 ,  G01N27/26

CPC分类号： G01N27/423 ,  G01N33/1806

摘要： The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance wherein the coulometric analysis has high reproducibility without any application of voltage, etc., from an outside source, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.

摘要翻译： 本发明提供了用于定量测定样品物质的快速库仑分析，其中库仑分析具有高的再现性，而不需要外部来源的电压等的施加，以及用于所述的电池的电池和装置 库仑分析 根据本发明，可以通过选择引入到原电池中的电活性物质来分析各种各样的组分。 本发明能够简单易用地分析谷物酸，抗坏血酸等食品成分，COD等水质等。

公开(公告)号：US20080138904A1

公开(公告)日：2008-06-12

申请号：US11793145

申请日：2005-12-12

申请人： Shigeru Nakano ,  Masahiro Fukaya

发明人： Shigeru Nakano ,  Masahiro Fukaya

IPC分类号： C12N15/09 ,  C12N1/21

CPC分类号： C12P7/40 ,  C12N1/00 ,  C12N15/52 ,  C12P7/52 ,  C12P7/54

摘要： It is intended to provide a method of conferring to microbial cells or the like, tolerance to a short chain fatty acid, including formic acid and acetic acid, which is harmful to the growth thereof without suppressing the growth of the cells while maintaining a high productivity of useful substances. It is also intended to provide a means for efficiently culturing a microorganism in the presence of a short chain fatty acid, or a means for producing a useful short chain fatty acid via fermentation.The present invention provides a method of breeding cells to improve tolerance to a short chain fatty acid, wherein a gene encoding a protein having a function of transporting a short chain fatty acid from the inside of cells to the outside of cells, is transformed into and expressed in the cells; a high cell-density culture method for producing useful substances using the cell; and a method of preparing a fermentation broth comprising a short chain fatty acid, wherein the cells are cultured under the conditions such that the cells produce a short chain fatty acid.

摘要翻译： 旨在提供赋予微生物细胞等的方法，对包含甲酸和乙酸的短链脂肪酸的耐受性，其对生长有害，而不抑制细胞生长，同时保持高生产率 的有用物质。 还提供了在短链脂肪酸存在下有效培养微生物的方法，或者是通过发酵生产有用的短链脂肪酸的方法。 本发明提供一种育种细胞以改善对短链脂肪酸耐受性的方法，其中将编码具有从细胞内部输送到外部的短链脂肪酸的功能的蛋白质的基因转化到细胞外， 在细胞中表达; 用于使用该细胞产生有用物质的高细胞密度培养方法; 以及制备包含短链脂肪酸的发酵液的方法，其中所述细胞在使细胞产生短链脂肪酸的条件下培养。

公开(公告)号：US4358494A

公开(公告)日：1982-11-09

申请号：US104865

申请日：1979-12-18

申请人： Saburo Akimoto ,  Sachio Maruchi ,  Masahiro Fukaya ,  Tomishi Shibano ,  Ippei Chimura ,  Hiroshi Asakura ,  Keisuke Kitazume ,  Shiro Fuse

发明人： Saburo Akimoto ,  Sachio Maruchi ,  Masahiro Fukaya ,  Tomishi Shibano ,  Ippei Chimura ,  Hiroshi Asakura ,  Keisuke Kitazume ,  Shiro Fuse

IPC分类号： B32B27/10 ,  C09J7/02 ,  B32B7/02

CPC分类号： B32B27/10 ,  C09J7/0296 ,  C09J2400/283 ,  Y10S428/906 ,  Y10T428/1452 ,  Y10T428/24942 ,  Y10T428/264 ,  Y10T428/277 ,  Y10T428/28 ,  Y10T428/2809 ,  Y10T428/2839 ,  Y10T428/2848 ,  Y10T428/31913

摘要： A widthwise-stretched film is lap-sealed on one side of a backing paper, and polyethylene is extruded and coated on the surface of said film to form a release layer thereon. A pressure-sensitive adhesive composed mainly of polyacrylic ester is then coated on the other side of the backing paper. In this manner, a paper-backed adhesive tape of the pressure-sensitive type is prepared.

摘要翻译： 将宽度方向拉伸的薄膜在背衬纸的一面上重叠密封，并将聚乙烯挤出并涂覆在所述薄膜的表面上以在其上形成剥离层。 然后将主要由聚丙烯酸酯组成的压敏粘合剂涂覆在背纸的另一侧上。 以这种方式制备压敏型纸背胶带。