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公开(公告)号：US5593963A
公开(公告)日：1997-01-14
申请号：US146424
申请日：1993-11-02
申请人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter C. Sijmons , Teunis C. Verwoerd
发明人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter C. Sijmons , Teunis C. Verwoerd
CPC分类号： A61K38/465 , A23K10/10 , A23K10/30 , A23K20/189 , C12N15/8242 , C12N9/16 , C12Y301/03026
摘要： The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.
摘要翻译： 本发明提供了植酸酶在转基因植物或植物器官中的表达及其生产方法。 提供DNA表达构建体用于在能够指导植酸酶表达的调控序列的控制下用编码植酸酶的基因转化植物。 这些调节序列包括根据植物或其部分的用途能够在植物中指导转录的序列，组成型或阶段和/或组织特异性的序列。 本发明提供的转基因植物和植物器官可以直接应用于各种工业过程，例如， 在动物饲料中，或者，可以提取表达的肌醇六磷酸酶，如果需要，在施用前进行纯化。
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公开(公告)号：US5543576A
公开(公告)日：1996-08-06
申请号：US146422
申请日：1993-11-02
申请人： Albert J. J. van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter C. Sijmons , Teunis C. Verwoerd , Wilhemus J. Quax
发明人： Albert J. J. van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter C. Sijmons , Teunis C. Verwoerd , Wilhemus J. Quax
CPC分类号： C12Y301/03026 , A23K20/189 , C12N15/8242 , C12N15/8243 , C12N15/8257 , C12N9/16 , C12N9/2417
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
摘要翻译： 公开了使用含有增加量的酶的种子催化体外反应的方法。 该方法包括将优选以研磨形式的转基因非野生型种子加入到反应混合物中并允许种子中的酶增加反应速率。 通过将种子直接加入到反应混合物中，该方法提供了提取和纯化酶的昂贵且有问题的方法的解决方案。 还提供了治疗方法，其中缺乏足够供应酶的受试者以含有增加量的酶的种子的形式施用酶。
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公开(公告)号：US5770413A
公开(公告)日：1998-06-23
申请号：US693709
申请日：1996-08-07
申请人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd
发明人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd
IPC分类号： A01H1/00 , A01H5/00 , A23K1/14 , A23K1/16 , A23K1/165 , C12N1/21 , C12N5/10 , C12N9/16 , C12N9/28 , C12N15/00 , C12N15/09 , C12N15/55 , C12N15/82 , C12P3/00 , C12P7/02 , C12P7/18 , C12R1/01 , C12R1/66
CPC分类号： C12Y301/03026 , A23K20/189 , C12N15/8242 , C12N15/8243 , C12N15/8257 , C12N9/16 , C12N9/2417
摘要： The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.
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公开(公告)号：US5714474A
公开(公告)日：1998-02-03
申请号：US626554
申请日：1996-04-02
申请人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd , Wilhemus Johannes Quax
发明人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd , Wilhemus Johannes Quax
CPC分类号： C12Y301/03026 , A23K20/189 , C12N15/8242 , C12N15/8243 , C12N15/8257 , C12N9/16 , C12N9/2417
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
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公开(公告)号：US6022846A
公开(公告)日：2000-02-08
申请号：US97847
申请日：1998-06-15
申请人： Albert J. J. Van Ooijen , Krun Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd
发明人： Albert J. J. Van Ooijen , Krun Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd
摘要： The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.
摘要翻译： 本发明提供了植酸酶在转基因植物或植物器官中的表达及其生产方法。 提供DNA表达构建体用于在能够指导植酸酶表达的调控序列的控制下用编码植酸酶的基因转化植物。 这些调节序列包括根据植物或其部分的用途能够在植物中指导转录的序列，组成型或阶段和/或组织特异性的序列。 本发明提供的转基因植物和植物器官可以直接应用于各种工业过程，例如， 在动物饲料中，或者，可以提取表达的肌醇六磷酸酶，如果需要，在施用前进行纯化。
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公开(公告)号：US20060195940A1
公开(公告)日：2006-08-31
申请号：US11193968
申请日：2005-07-30
申请人： Albert Van Ooyen , Krijn Rietveld , Wilhelmus Quax , Petrus Van Den Elzen , Jan Pen , Andreas Hoekema , Peter Sijmons
发明人： Albert Van Ooyen , Krijn Rietveld , Wilhelmus Quax , Petrus Van Den Elzen , Jan Pen , Andreas Hoekema , Peter Sijmons
CPC分类号： C12N9/2411 , A01H1/00 , C12N9/2408 , C12N9/2414 , C12N9/2417 , C12N9/242 , C12N9/2428 , C12N15/8245
摘要： The present invention provides plants with a modified taste, solids content and/or texture. The invention also provides methods of obtaining such plants via transformation with DNA constructs containing genes encoding enzymes capable of degrading plant polysaccharides and optionally additional genes encoding enzymes which are capable of further modifying the degradation products resulting from the first degradation step.
摘要翻译： 本发明为植物提供改良的味道，固体含量和/或质地。 本发明还提供了通过使用含有能够降解植物多糖的酶的基因的DNA构建体和任选地能够进一步修饰由第一降解步骤产生的降解产物的另外的编码酶的基因进行转化来获得这样的植物的方法。
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公开(公告)号：US5705375A
公开(公告)日：1998-01-06
申请号：US253575
申请日：1994-06-03
申请人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Petrus Josephus Maria Van Den Elzen , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons
发明人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Petrus Josephus Maria Van Den Elzen , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons
CPC分类号： C12N15/8245
摘要： The present invention provides plants with a modified taste, solids content and/or texture. The invention also provides methods of obtaining such plants via transformation with DNA constructs containing genes encoding enzymes capable of degrading plant polysaccharides and optionally additional genes encoding enzymes which are capable of further modifying the degradation products resulting from the first degradation step.
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公开(公告)号：US07033627B2
公开(公告)日：2006-04-25
申请号：US09149310
申请日：1998-02-02
申请人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons , Teunis Cornelis Verwoerd
发明人： Albert Johannes Joseph Van Ooyen , Krijn Rietveld , Wilhelmus Johannes Quax , Jan Pen , Andreas Hoekema , Peter Christiaan Sijmons , Teunis Cornelis Verwoerd
CPC分类号： C12Y301/03026 , A21D8/042 , A23K20/189 , A23L29/06 , C12N9/16 , C12N9/2417 , C12N15/8242 , C12N15/8243 , C12N15/8257
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
摘要翻译： 公开了使用含有增加量的酶的种子催化体外反应的方法。 该方法包括将转基因非野生型种子，优选以研磨形式加入到反应混合物，特别是面包店方法中，并允许种子中的酶提高反应速率。 通过将种子直接加入到反应混合物中，该方法提供了提取和纯化酶的昂贵且有问题的方法的解决方案。 还提供了治疗方法，其中缺乏足够供应酶的受试者以含有增加量的酶的种子的形式施用酶。
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公开(公告)号：US20060005286A1
公开(公告)日：2006-01-05
申请号：US11214413
申请日：2005-08-29
申请人： Albert Van Ooyen , Krijn Rietveld , Wilhelmus Quax , Jan Pen , Andreas Hoekema , Peter Sijmons , Teunis Verwoerd
发明人： Albert Van Ooyen , Krijn Rietveld , Wilhelmus Quax , Jan Pen , Andreas Hoekema , Peter Sijmons , Teunis Verwoerd
CPC分类号： C12Y301/03026 , A21D8/042 , A23K20/189 , A23L29/06 , C12N9/16 , C12N9/2417 , C12N15/8242 , C12N15/8243 , C12N15/8257
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture, especially a bakery process and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the mehtod provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
摘要翻译： 公开了使用含有增加量的酶的种子催化体外反应的方法。 该方法包括将转基因非野生型种子，优选以研磨形式加入到反应混合物，特别是面包店方法中，并允许种子中的酶提高反应速率。 通过将种子直接加入到反应混合物中，mehtod提供了解决提取和纯化酶的昂贵和有问题的过程的解决方案。 还提供了治疗方法，其中缺乏足够供应酶的受试者以含有增加量的酶的种子的形式施用酶。
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公开(公告)号：US5925804A
公开(公告)日：1999-07-20
申请号：US569150
申请日：1995-12-21
IPC分类号： A01N3/00 , A23L3/3562 , A23L3/42 , C12N1/21 , C12N5/10 , C12N9/10 , C12N9/12 , C12N15/11 , C12N15/54 , C12N15/82 , C12P19/12 , A01H5/00 , A01H5/10 , C12N5/14 , C12N15/31 , C12N15/52
CPC分类号： C12N9/1051 , A23L3/3562 , A23L3/42 , C12N15/8245 , C12N9/1066 , C12N9/12
摘要： A nucleic acid having (i) a DNA molecule which, when expressed in a plant or plant cell, increases the trehalose content of the plant or plant cell, the DNA molecule encoding an E. coli trehalose phosphate synthase, and (ii) a plant expressible promoter operatively coupled to the DNA molecule. Also, a method for obtaining a plant with increased trehalose production by introducing into a recipient cell of a plant, a plant expressible gene which, when expressed in a plant or plant cell increases the trehalose content of the plant or plant cell. The plant expressible gene is an E. coli trehalose phosphate synthase gene which is operably linked to: a) a transcriptional initiation region that is functional in the plant, and b) a DNA molecule encoding a selectable marker gene that is functional in the plant. The method includes a step of regenerating a plant from the recipient cell under conditions that allow for selection for the presence of the selectable marker gene.
摘要翻译： PCT No.PCT / EP94 / 02167 371 1995年12月21日第 102（e）日期1995年12月21日PCT提交1994年6月30日PCT公布。 公开号WO95 / 01446 日期1995年1月12日一种具有（i）DNA分子的核酸，其在植物或植物细胞中表达时，增加植物或植物细胞的海藻糖含量，编码大肠杆菌海藻糖磷酸合成酶的DNA分子，以及 （ii）可操作地偶联于DNA分子的植物可表达启动子。 此外，通过将植物或植物细胞的海藻糖含量提高到植物的受体细胞中可获得具有增加的海藻糖产生的植物的方法，当在植物或植物细胞中表达时植物可表达基因增加。 植物可表达基因是大肠杆菌海藻糖磷酸合酶基因，其可操作地连接到：a）在植物中有功能的转录起始区，和b）编码在植物中有功能的可选择标记基因的DNA分子。 该方法包括在允许选择存在选择性标记基因的条件下从受体细胞再生植物的步骤。
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