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公开(公告)号：US08658093B2
公开(公告)日：2014-02-25
申请号：US12167292
申请日：2008-07-03
IPC分类号： G01N1/26
CPC分类号： G01N33/54366 , B01L3/5027 , B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/069 , B01L2300/0816 , B01L2300/0864 , G01N33/54326 , G01N2400/00
摘要： System and methods for detecting analytes such as pathogenic cells are described. The methods allow for the direct measurement of analytes such as pathogenic organisms without the need for sample preparation and/or PCR. The devices can be used individually as point-of-use sensors for airborne pathogens and other pathogenic organisms in foods and agriculture products.
摘要翻译： 描述了用于检测分析物如致病细胞的系统和方法。 这些方法允许直接测量分析物如病原体，而不需要样品制备和/或PCR。 这些装置可以单独用作食品和农产品中的空气传播病原体和其他致病生物的使用点传感器。
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公开(公告)号：US20090142772A1
公开(公告)日：2009-06-04
申请号：US12167292
申请日：2008-07-03
CPC分类号： G01N33/54366 , B01L3/5027 , B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/069 , B01L2300/0816 , B01L2300/0864 , G01N33/54326 , G01N2400/00
摘要： System and methods for detecting analytes such as pathogenic cells are described. The methods allow for the direct measurement of analytes such as pathogenic organisms without the need for sample preparation and/or PCR. The devices can be used individually as point-of-use sensors for airborne pathogens and other pathogenic organisms in foods and agriculture products.
摘要翻译： 描述了用于检测分析物如致病细胞的系统和方法。 这些方法允许直接测量分析物如病原体，而不需要样品制备和/或PCR。 这些装置可以单独用作食品和农产品中的空气传播病原体和其他致病生物的使用点传感器。
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公开(公告)号：US20110318740A1
公开(公告)日：2011-12-29
申请号：US13149697
申请日：2011-05-31
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6846 , C12Q2522/101 , C12Q2561/109 , C12Q2565/519 , C12Q1/686
摘要： Disclosed, for example, are methods for detecting at least one target polynucleotide comprising cleaving a flap from a polynucleotide probe that is hybridized to a complementary target polynucleotide, and hybridizing the cleaved flap to a complementary capture probe immobilized on a surface, wherein said cleaving and/or hybridizing occurs in the presence of a single strand binding protein that is capable of binding single-stranded DNA, but that can bind neither the flap nor the capture probe. The cleaved flap and the complementary capture probe may each comprise PNA, RNA, LNA, L-DNA or other modified nucleotides or chimeras thereof that do not significantly bind to the single strand binding protein.
摘要翻译： 例如，公开了用于检测至少一种靶多核苷酸的方法，所述至少一种靶多核苷酸包括从互补靶多核苷酸杂交的多核苷酸探针切割瓣，并将切割的瓣与固定在表面上的互补捕获探针杂交，其中所述切割和 /或杂交发生在能够结合单链DNA的单链结合蛋白存在下，但是既不结合皮瓣也不能结合捕获探针。 切割的瓣和互补捕获探针可以各自包含不显着结合单链结合蛋白的PNA，RNA，LNA，L-DNA或其它修饰的核苷酸或嵌合体。
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公开(公告)号：US08492158B2
公开(公告)日：2013-07-23
申请号：US12059744
申请日：2008-03-31
申请人： Robert G. Eason
发明人： Robert G. Eason
IPC分类号： G01N33/20
CPC分类号： C07H15/26 , C12Q1/6816 , C12Q2563/137
摘要： Disclosed are tri-nuclear metal complexes and salts thereof, such as tri-nuclear osmium or ruthenium complexes or salts thereof, suitable for use as electrochemical labels. Also disclosed are oligonucleotide probes with an attached electrochemical label, methods of nucleic acid amplification, methods of sequencing, and kits for nucleic acid amplification and sequencing having oligonucleotide probes including an electrochemical label. The electrochemical labels are synthesized from siderophores.
摘要翻译： 公开了适合用作电化学标记的三核金属络合物及其盐，例如三核锇或钌络合物或其盐。 还公开了具有附加的电化学标记的寡核苷酸探针，核酸扩增方法，测序方法，以及用于核酸扩增和测序的试剂盒，其具有包含电化学标记的寡核苷酸探针。 电化学标记由铁载体合成。
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公开(公告)号：US09127306B2
公开(公告)日：2015-09-08
申请号：US12372464
申请日：2009-02-17
CPC分类号： C12Q1/6806 , C07H1/06 , C07H21/00 , C12N15/1093 , C12Q2523/301
摘要： Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.
摘要翻译： 提供了用于从纯化的核酸样品制备核酸片段的方法和试剂盒。 或者，染色质或其他长聚合物可以用类似的方法和试剂盒进行剪切。
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公开(公告)号：US20090246763A1
公开(公告)日：2009-10-01
申请号：US12059744
申请日：2008-03-31
申请人： Robert G. Eason
发明人： Robert G. Eason
IPC分类号： C12Q1/68 , C07D259/00 , C07H15/26 , C07F15/00
CPC分类号： C07H15/26 , C12Q1/6816 , C12Q2563/137
摘要： Disclosed are tri-nuclear metal complexes and salts thereof, such as tri-nuclear osmium or ruthenium complexes or salts thereof, suitable for use as electrochemical labels. Also disclosed are oligonucleotide probes with an attached electrochemical label, methods of nucleic acid amplification, methods of sequencing, and kits for nucleic acid amplification and sequencing having oligonucleotide probes including an electrochemical label. The electrochemical labels are synthesized from siderophores.
摘要翻译： 公开了适合用作电化学标记的三核金属络合物及其盐，例如三核锇或钌络合物或其盐。 还公开了具有附加的电化学标记的寡核苷酸探针，核酸扩增方法，测序方法，以及用于核酸扩增和测序的试剂盒，其具有包含电化学标记的寡核苷酸探针。 电化学标记由铁载体合成。
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7.发明授权Synthesis of 2′,3′-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization 有权用于自动DNA合成和焦磷酸裂解活化聚合的2'，3'-双脱氧核苷合成公开(公告)号：US08658776B2
公开(公告)日：2014-02-25
申请号：US13117029
申请日：2011-05-26
IPC分类号： C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C07D239/54
CPC分类号： C07H19/16 , C07D239/54 , C07D405/04 , C07D473/18 , C07D473/34 , C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6806
摘要： Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
摘要翻译： 公开了制备2'，3'-二脱氧核苷酸支持结构的方法，例如2'，3'-二脱氧鸟苷，2'，3'-二脱氧腺苷和3'-脱氧胸苷支持结构。 还提供了使用这些结构的各种方法，例如其用于自动DNA合成和焦磷酸裂解活化聚合的用途。
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公开(公告)号：US20090233814A1
公开(公告)日：2009-09-17
申请号：US12372464
申请日：2009-02-17
CPC分类号： C12Q1/6806 , C07H1/06 , C07H21/00 , C12N15/1093 , C12Q2523/301
摘要： Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.
摘要翻译： 提供了用于从纯化的核酸样品制备核酸片段的方法和试剂盒。 或者，染色质或其他长聚合物可以用类似的方法和试剂盒进行剪切。
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