公开(公告)号：US08752200B2

公开(公告)日：2014-06-10

申请号：US13180805

申请日：2011-07-12

申请人： Alexander Varshavsky ,  Kevin Ansia Li

发明人： Alexander Varshavsky ,  Kevin Ansia Li

IPC分类号： H04L9/32 ,  H04L12/22

CPC分类号： H04L63/0861 ,  G06F21/32 ,  G06F21/44 ,  G06F2221/2111 ,  H04L9/3231 ,  H04L9/3247 ,  H04L2209/805 ,  H04W12/06

摘要： Devices, systems and methods are disclosed for determining an electromagnetic signature for authenticating a device, a user, and/or a location. In exemplary embodiments, a magnetometer captures an electromagnetic signature which is then compared with one or more authorized electromagnetic signatures. If the electromagnetic signature matches an authorized electromagnetic signature, then access is granted. The magnetometer is integrated into a communication device having a processor and a logic. The magnetometer captures an electromagnetic signature of a surrounding environment and detects motion of the communication device through the captured electromagnetic signature. The logic on the communication device locks or unlocks features of the device based upon the captured electromagnetic signature. In further embodiments of the subject disclosure, the magnetometer is in communication with a server which authenticates a user or communication device to provide access to a remote location.

摘要翻译： 公开了用于确定用于认证设备，用户和/或位置的电磁签名的设备，系统和方法。 在示例性实施例中，磁力计捕获电磁签名，然后与一个或多个授权的电磁签名进行比较。 如果电磁签名符合授权的电磁签名，则授权访问。 磁力计集成到具有处理器和逻辑的通信设备中。 磁力计捕获周围环境的电磁特征，并通过捕获的电磁特征检测通信设备的运动。 通信设备上的逻辑基于所捕获的电磁签名来锁定或解锁设备的特征。 在本发明的进一步实施例中，磁力计与服务器进行通信，服务器认证用户或通信设备以提供对远程位置的访问。

公开(公告)号：US20130019292A1

公开(公告)日：2013-01-17

申请号：US13180805

申请日：2011-07-12

申请人： Alexander Varshavsky ,  Kevin Ansia Li

发明人： Alexander Varshavsky ,  Kevin Ansia Li

IPC分类号： H04L9/32 ,  G06F15/16 ,  G06F21/00

CPC分类号： H04L63/0861 ,  G06F21/32 ,  G06F21/44 ,  G06F2221/2111 ,  H04L9/3231 ,  H04L9/3247 ,  H04L2209/805 ,  H04W12/06

摘要： Devices, systems and methods are disclosed for determining an electromagnetic signature for authenticating a device, a user, and/or a location. In exemplary embodiments, a magnetometer captures an electromagnetic signature which is then compared with one or more authorized electromagnetic signatures. If the electromagnetic signature matches an authorized electromagnetic signature, then access is granted. The magnetometer is integrated into a communication device having a processor and a logic. The magnetometer captures an electromagnetic signature of a surrounding environment and detects motion of the communication device through the captured electromagnetic signature. The logic on the communication device locks or unlocks features of the device based upon the captured electromagnetic signature. In further embodiments of the subject disclosure, the magnetometer is in communication with a server which authenticates a user or communication device to provide access to a remote location.

摘要翻译： 公开了用于确定用于认证设备，用户和/或位置的电磁签名的设备，系统和方法。 在示例性实施例中，磁力计捕获电磁签名，然后与一个或多个授权的电磁签名进行比较。 如果电磁签名符合授权的电磁签名，则授权访问。 磁力计集成到具有处理器和逻辑的通信设备中。 磁力计捕获周围环境的电磁特征，并通过捕获的电磁特征检测通信设备的运动。 通信设备上的逻辑基于所捕获的电磁签名来锁定或解锁设备的特征。 在本发明的进一步实施例中，磁力计与服务器进行通信，服务器认证用户或通信设备以提供对远程位置的访问。

公开(公告)号：US08873755B2

公开(公告)日：2014-10-28

申请号：US13111237

申请日：2011-05-19

申请人： Suhas Mathur ,  Wade Trappe ,  Alexander Varshavsky

发明人： Suhas Mathur ,  Wade Trappe ,  Alexander Varshavsky

IPC分类号： H04L9/00 ,  H04L9/08 ,  H04W12/04

CPC分类号： H04L9/0875 ,  H04L2209/34 ,  H04L2209/80 ,  H04W12/04

摘要： Wireless devices in proximity are securely paired with one another autonomously by generating a common cryptographic key directly from a time-varying wireless environment shared among the wireless devices. The shared key can be used by the wireless devices to authenticate each wireless device's physical proximity and then to facilitate confidential communication between the wireless devices. The algorithm used to create the shared key is secure against a computationally unbounded adversary and its computational complexity is linear in the size of the shared key.

摘要翻译： 邻近的无线设备通过从无线设备之间共享的时变无线环境直接生成公用密码密钥而自主地彼此安全地配对。 无线设备可以使用共享密钥来认证每个无线设备的物理接近度，并且便于无线设备之间的机密通信。 用于创建共享密钥的算法对于计算上无限制的对手是安全的，并且其计算复杂度在共享密钥的大小上是线性的。

公开(公告)号：US20060084097A1

公开(公告)日：2006-04-20

申请号：US11228157

申请日：2005-09-16

申请人： Rong-Gui Hu ,  Jun Sheng ,  Yong Kwon ,  Anna Kashina ,  Alexander Varshavsky

发明人： Rong-Gui Hu ,  Jun Sheng ,  Yong Kwon ,  Anna Kashina ,  Alexander Varshavsky

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C40B40/08 ,  C40B40/10

CPC分类号： C12Q1/48 ,  G01N2333/91074 ,  G01N2333/91188 ,  G01N2500/04

摘要： Screening assays that allow for the identification of agents that modulate the activity of the arginylation branch of the N-end rule pathway are provided. Also provided are method of using an agent that modulate the activity of the arginylation branch of the N-end rule pathway to increase or decrease protein degradation in a cell, and to modulate physiologic and pathologic associated with N-end rule pathway mediated arginylation.

公开(公告)号：US5494818A

公开(公告)日：1996-02-27

申请号：US5002

申请日：1993-01-15

申请人： Rohan T. Baker ,  John W. Tobias ,  Alexander Varshavsky

发明人： Rohan T. Baker ,  John W. Tobias ,  Alexander Varshavsky

IPC分类号： C12N15/09 ,  C07K14/00 ,  C12N1/21 ,  C12N9/52 ,  C12N9/60 ,  C12N15/57 ,  C12N15/62 ,  C12P21/06 ,  C12R1/19 ,  C12N9/50 ,  C12N9/58 ,  C12N9/64

CPC分类号： C12P21/06 ,  C07K14/00 ,  C12N15/62 ,  C12N9/60 ,  C07K2319/00 ,  C07K2319/95

摘要： The disclosure relates to a generic class of ubiquitin-specific proteases which specifically cleave at the C-terminus of the ubiquitin moiety in a ubiquitin fusion protein irrespective of the size of the ubiquitin fusion protein. More specifically, the disclosure relates to ubiquitin-specific proteases of this class which have been isolated from a cell. The disclosure also relates to isolated DNA sequences encoding the proteases of this class.

摘要翻译： 本公开涉及泛素特异性蛋白酶的泛型，其泛素泛素融合蛋白中泛素部分的C末端特异性切割，而与泛素融合蛋白的大小无关。 更具体地，本公开涉及已经从细胞中分离的该类的泛素特异性蛋白酶。 本公开还涉及编码该类蛋白酶的分离的DNA序列。

公开(公告)号：US5196321A

公开(公告)日：1993-03-23

申请号：US821720

申请日：1992-01-15

申请人： Andreas Bachmair ,  Daniel Finley ,  Alexander Varshavsky

发明人： Andreas Bachmair ,  Daniel Finley ,  Alexander Varshavsky

IPC分类号： C07K1/107 ,  C07K14/00 ,  C12N9/06 ,  C12N9/38 ,  C12N9/60 ,  C12N9/64 ,  C12N15/11 ,  C12N15/62 ,  C12N15/67 ,  C12P21/02 ,  C12P21/04

CPC分类号： C12N9/0028 ,  C07K1/107 ,  C07K1/1075 ,  C07K14/00 ,  C12N15/11 ,  C12N15/62 ,  C12N15/67 ,  C12N9/2471 ,  C12N9/60 ,  C12N9/6421 ,  C12P21/02 ,  C12Y302/01023 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/61 ,  C07K2319/95

摘要： Methods of designing or modifying protein structure at the protein or genetic level to produce specified amino-termini in vivo or in vitro are described. The methods can be used to alter the metabolic stability and other properties of the protein or, alternatively, to artificially generate authentic amino-termini in proteins produced through artificial means. The methods are based upon the introduction of the use of artificial ubiquitin-protein fusions, and the discovery that the in vivo half-life of a protein is a function of the amino-terminal amino acid of the protein.

摘要翻译： 描述了在蛋白质或遗传水平上设计或修饰蛋白质结构以在体内或体外产生特定氨基末端的方法。 该方法可用于改变蛋白质的代谢稳定性和其它性质，或者替代地，通过人工方式人造产生的蛋白质中的正确的氨基末端。 该方法基于引入人造泛素 - 蛋白质融合物的使用，并且发现蛋白质的体内半衰期是蛋白质的氨基末端氨基酸的函数。

公开(公告)号：US5861312A

公开(公告)日：1999-01-19

申请号：US982956

申请日：1997-12-02

申请人： Alexander Varshavsky ,  Yong Tae Kwon

发明人： Alexander Varshavsky ,  Yong Tae Kwon

IPC分类号： C12N15/09 ,  A61K31/7088 ,  A61K45/00 ,  A61K48/00 ,  A61P21/00 ,  C07K14/47 ,  C12N1/15 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N15/12 ,  C12N15/63

CPC分类号： C12N9/93 ,  C12Y603/02019

摘要： Disclosed here is a nucleic acid sequence encoding a recognition component of the N-end rule pathway. This nucleic acid sequence is characterized by the ability to specifically hybridize to the nucleic acid sequence of SEQ ID NO 1 under stringent hybridization conditions. Such conditions are defined below. Also disclosed is a nucleic acid sequence encoding a recognition component of the N-end rule pathway which is characterized by the ability to specifically hybridize to the nucleic acid sequence of SEQ ID NO 2 under stringent hybridization conditions. Also disclosed are DNA expression vectors containing nucleic acid sequences of the type described above, as well as cells transformed with such expression vectors. Further disclosed are applications for the compositions described above.

摘要翻译： 这里公开了编码N末端规则途径的识别成分的核酸序列。 该核酸序列的特征在于在严格杂交条件下与SEQ ID NO 1的核酸序列特异性杂交的能力。 这些条件定义如下。 还公开了编码N末端规则通路的识别组分的核酸序列，其特征在于在严格杂交条件下与SEQ ID NO 2的核酸序列特异性杂交的能力。 还公开了含有上述类型的核酸序列的DNA表达载体以及用这种表达载体转化的细胞。 进一步公开的是上述组合物的应用。

公开(公告)号：US5766927A

公开(公告)日：1998-06-16

申请号：US230453

申请日：1994-04-20

申请人： Rohan T. Baker ,  David K. Gonda ,  Alexander Varshavsky

发明人： Rohan T. Baker ,  David K. Gonda ,  Alexander Varshavsky

IPC分类号： A61K38/00 ,  A61P43/00 ,  C07K2/00 ,  C07K4/00 ,  C07K5/06 ,  C12N9/00 ,  C12N9/38 ,  C12N15/09 ,  C12N15/62 ,  C12N15/67 ,  C12N15/81 ,  C12P21/00 ,  C12P21/02 ,  C12R1/865 ,  C12N1/16 ,  A61K38/05 ,  C12N1/38

CPC分类号： C12P21/02 ,  C12N15/62 ,  C12N15/67 ,  C12N15/81 ,  C12N9/2471 ,  C12Y302/01023 ,  C07K2319/00 ,  C07K2319/61 ,  C07K2319/95

摘要： The half-life of a Type I, II and III non-compartmentalized intracellular proteins is increased in living eukaryotic cells by contacting the cells with a regulator having an amino-terminal amino acid residue which is the same or similar to the amino-terminal residue of the intracellular protein. The regulator is a dipeptide, a small polypeptide or a carboxyl-terminal derivative of an amino acid. The dipeptide or small polypeptide has an N-terminal amino acid residue which is Arg, Lys or His for the Type I protein, Phe, Leu, Trp, Tyr or Ile for the Type II protein and Ala, Ser or Thr for the Type III protein. The carboxyl-terminal derivative of an amino acid may be an amino acid modified at its C-terminus by the addition of a group selected from methyl, ethyl, propyl, butyl and isobutyl. The amino acid modified is the N-terminal amino acid residue of the dipeptide or small polypeptide for the respective Type I, II and III proteins. Compositions may be formed containing the regulator for contacting with the cells. Increasing the half-life of intracellular protein with the regulator may be used for treating diseases resulting from an abnormal breakdown of a desired protein, and for enhancing in vivo production of a desired protein.

摘要翻译： 通过使细胞与具有与氨基末端残基相同或相似的氨基末端氨基酸残基的调节剂接触，生物真核细胞中I型，II型和III型非分隔细胞内蛋白质的半衰期增加 的细胞内蛋白质。 调节剂是氨基酸的二肽，小多肽或羧基末端衍生物。 二肽或小多肽具有N-末端氨基酸残基，其为II型蛋白的I型蛋白，Phe，Leu，Trp，Tyr或Ile的Arg，Lys或His，以及III型的Ala，Ser或Thr 蛋白。 氨基酸的羧基末端衍生物可以是通过加入选自甲基，乙基，丙基，丁基和异丁基的基团在其C末端修饰的氨基酸。 修饰的氨基酸是相应的I，II和III型蛋白质的二肽或小多肽的N-末端氨基酸残基。 可以形成含有与细胞接触的调节剂的组合物。 用调节剂增加细胞内蛋白质的半衰期可用于治疗由所需蛋白质的异常分解引起的疾病，以及用于增强所需蛋白质的体内产生。

公开(公告)号：US5538862A

公开(公告)日：1996-07-23

申请号：US192479

申请日：1994-02-04

申请人： Peipei Wu ,  Jurgen Dohmen ,  Alexander Varshavsky

发明人： Peipei Wu ,  Jurgen Dohmen ,  Alexander Varshavsky

IPC分类号： C12N15/09 ,  C07K14/00 ,  C07K14/395 ,  C07K14/47 ,  C07K19/00 ,  C12N1/21 ,  C12N9/02 ,  C12N9/06 ,  C12N9/12 ,  C12N15/11 ,  C12N15/62 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/865 ,  C12P21/06 ,  C07H19/00 ,  C07H21/00

CPC分类号： C12N9/0028 ,  C07K14/00 ,  C07K14/001 ,  C07K14/395 ,  C12N15/11 ,  C12N15/62 ,  C12N9/003 ,  C12N9/12 ,  C07K2319/00 ,  C07K2319/42 ,  C07K2319/61 ,  C07K2319/95

摘要： The N-degron is an intracellular degradation signal whose essential determinant is a specific, destabilizing, N-terminal amino acid residue. A set of N-degrons containing different destabilizing residues is manifested as the N-end rule, which relates the in vivo half-life of a protein to the identity of its N-terminal amino acid residue. Disclosed herein is a heat-inducible N-degron module. A heat-inducible N-degron module is a protein or peptide bearing a destabilizing N-terminal amino acid residue which becomes a substrate of the N-end rule pathway only at a temperature high enough to result in at least partial unfolding of the protein. At this elevated (nonpermissive) temperature, the heat-inducible N-degron module (and any protein or peptide attached at its C-terminus) is rapidly degraded in a cell in which the N-end rule pathway is operative. Also disclosed are DNA and protein fusion constructs, methods for screening for additional heat-inducible N-degron modules and methods for using the disclosed heat-inducible N-degron modules.

摘要翻译： N-degron是细胞内降解信号，其主要决定簇是特异的，不稳定的N-末端氨基酸残基。 含有不同去稳定残基的一组N-解码子表现为N端规则，其将蛋白质的体内半衰期与其N-末端氨基酸残基的身份相关联。 本文公开了一种热诱导型N-离子解聚模块。 热诱导型N-解体模块是具有不稳定的N-末端氨基酸残基的蛋白质或肽，其仅在足以导致蛋白质的至少部分展开的温度下成为N端规则通路的底物。 在这种升高的（非允许的）温度下，在N端规则通路可操作的细胞中，热诱导型N-脱落元件模块（以及在其C-末端附着的任何蛋白质或肽）被快速降解。 还公开了DNA和蛋白质融合构建体，用于筛选额外的热诱导性N-脱落模块的方法以及使用所公开的热诱导型N-脱落伞模块的方法。

公开(公告)号：US5503977A

公开(公告)日：1996-04-02

申请号：US232815

申请日：1994-04-22

申请人： Nils Johnsson ,  Alexander Varshavsky

发明人： Nils Johnsson ,  Alexander Varshavsky

IPC分类号： C07K14/00 ,  C12Q1/37 ,  G01N33/532 ,  G01N33/542 ,  G01N33/543 ,  C07K19/00 ,  C12N15/62 ,  C12N15/63 ,  C12Q1/68

CPC分类号： G01N33/532 ,  C07K14/00 ,  C12Q1/37 ,  G01N33/542 ,  G01N33/54306 ,  C07K2319/00

摘要： Disclosed are novel compositions and methods useful for studying interactions between proteins. An N-terminal subdomain and a C-terminal subdomain of ubiquitin are linked to a pair of proteins or peptides to be examined for their ability to interact. When contacted with one another, a quasi-native ubiquitin moiety is reconstituted provided that the protein or peptide pair do, in fact, interact (bind) with one another. The quasi-native ubiquitin moiety is recognized and cleaved by ubiquitin-specific proteases after the last residue of ubiquitin. The cleavage at the quasi-native ubiquitin moiety within a linear protein fusion is the indication of interaction between the protein or peptide pair.

摘要翻译： 公开了用于研究蛋白质之间的相互作用的新型组合物和方法。 泛素的N-末端亚结构域和C末端亚结构域与一对待检查的蛋白质或肽的相互作用能力相关联。 当彼此接触时，重新制备准天然泛素部分，条件是蛋白质或肽对实际上相互作用（结合）。 在泛蛋白的最后残基之后，准天然泛素部分被泛素特异性蛋白酶识别和切割。 线性蛋白融合中准天然泛素部分的切割是蛋白质或肽对之间相互作用的指示。