公开(公告)号：US5843633A

公开(公告)日：1998-12-01

申请号：US639891

申请日：1996-04-26

申请人： Amy Yin ,  Sheri Miraglia ,  David W. Buck

发明人： Amy Yin ,  Sheri Miraglia ,  David W. Buck

IPC分类号： C12N15/02 ,  C07K14/47 ,  C07K14/705 ,  C07K16/18 ,  C07K16/28 ,  C12N5/10 ,  C12N5/20 ,  C12P21/08 ,  G01N33/53 ,  G01N33/577

CPC分类号： C07K16/28 ,  C07K14/705

摘要： An hematopoietic progenitor cell antigen, and antibodies that specifically bind to the antigen are provided. Expression of the antigen is highly tissue specific. It is only detected on a subset of hematopoietic progenitor cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34.sup.bright, and contains substantially all of the CFU-GM activity present in the CD34.sup.+ population. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells. Cells selected for expression of AC133 antigen may be further purified by selection for other hematopoietic stem cell and progenitor cell markers.

摘要翻译： 提供造血祖细胞抗原和特异性结合抗原的抗体。 抗原的表达是高度组织特异性的。 仅在衍生自人骨髓，胎儿骨髓和肝脏，脐带血和成人外周血的造血祖细胞的子集上检测到。 AC133识别的细胞亚群是CD34bright，并且包含存在于CD34 +群体中的基本上所有的CFU-GM活性。 这种高度特异性的AC133分布使其特别有用作分离和表征人类造血祖细胞和干细胞的试剂。 选择用于表达AC133抗原的细胞可以通过选择其它造血干细胞和祖细胞标记进一步纯化。

公开(公告)号：US08084033B2

公开(公告)日：2011-12-27

申请号：US12169336

申请日：2008-07-08

申请人： Amy H. Yin ,  Sheri Miraglia ,  Wayne G. Godfrey ,  David W. Buck

发明人： Amy H. Yin ,  Sheri Miraglia ,  Wayne G. Godfrey ,  David W. Buck

IPC分类号： A61K39/00 ,  A61K39/395 ,  C07K16/00 ,  C07K17/00 ,  C12P21/08

CPC分类号： C07K14/705 ,  C07K16/28 ,  G01N33/56966 ,  Y10S530/806 ,  Y10S530/838

摘要： Compositions comprising a cell expressing an AC133 cell surface antigen and an antibody, or antigen binding fragment thereof, that specifically binds to the antigen are provided. Expression of the antigen is highly tissue specific. It is detected on a subset of hematopoietic progenitor and stem cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34bright and contains substantially all of the CFU-GM activity present in the CD34+ population. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells. Cells selected for expression of AC133 antigen can be further purified by selection for other hematopoietic stem cell and progenitor cell markers.

摘要翻译： 提供了包含特异性结合抗原的表达AC133细胞表面抗原的细胞和抗体或其抗原结合片段的组合物。 抗原的表达是高度组织特异性的。 在源自人骨髓，胎儿骨髓和肝脏，脐带血和成人外周血的造血祖细胞和干细胞的子集上检测到。 由AC133识别的细胞亚群是CD34bright并且基本上包含存在于CD34 +群体中的所有CFU-GM活性。 这种高度特异性的AC133分布使其特别有用作分离和表征人类造血祖细胞和干细胞的试剂。 选择用于表达AC133抗原的细胞可以通过选择其他造血干细胞和祖细胞标记进一步纯化。

公开(公告)号：US20090093052A1

公开(公告)日：2009-04-09

申请号：US12169336

申请日：2008-07-08

申请人： Amy H. Yin ,  Sheri Miraglia ,  David W. Buck

发明人： Amy H. Yin ,  Sheri Miraglia ,  David W. Buck

IPC分类号： C12N5/06 ,  C12N5/00

CPC分类号： C07K14/705 ,  C07K16/28 ,  G01N33/56966 ,  Y10S530/806 ,  Y10S530/838

摘要： A hematopoietic progenitor cell antigen and reagents, notably antibodies, that specifically bind to the antigen are provided. Expression of the antigen is highly tissue specific. It is only detected on a subset of hematopoietic progenitor cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34bright and contains substantially all of the CFU-GM activity present in the CD34+ population. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells. Cells selected for expression of AC133 antigen can be further purified by selection for other hematopoietic stem cell and progenitor cell markers.

摘要翻译： 提供了特异性结合抗原的造血祖细胞抗原和试剂，特别是抗体。 抗原的表达是高度组织特异性的。 仅在衍生自人骨髓，胎儿骨髓和肝脏，脐带血和成人外周血的造血祖细胞的子集上检测到。 由AC133识别的细胞亚群是CD34bright并且基本上包含存在于CD34 +群体中的所有CFU-GM活性。 这种高度特异性的AC133分布使其特别有用作分离和表征人类造血祖细胞和干细胞的试剂。 选择用于表达AC133抗原的细胞可以通过选择其他造血干细胞和祖细胞标记进一步纯化。

公开(公告)号：US06455678B1

公开(公告)日：2002-09-24

申请号：US08842382

申请日：1997-04-23

申请人： Amy H. Yin ,  Sheri Miraglia ,  Wayne G. Godfry ,  David W. Buck

发明人： Amy H. Yin ,  Sheri Miraglia ,  Wayne G. Godfry ,  David W. Buck

IPC分类号： C07K100

CPC分类号： C07K14/705 ,  C07K16/28 ,  G01N33/56966 ,  Y10S530/806 ,  Y10S530/838

摘要： A hematopoietic progenitor cell antigen and reagents, notably antibodies, that specifically bind to the antigen are provided. Expression of the antigen is highly tissue specific. It is only detected on a subset of hematopoietic progenitor cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34bright and contains substantially all of the CFU-GM activity present in the CD34+ population. This highly specific distribution of AC133 makes it exceptionally useful as a reagent for isolating and characterizing human hematopoietic progenitor and stem cells. Cells selected for expression of AC133 antigen can be further purified by selection for other hematopoietic stem cell and progenitor cell markers.

摘要翻译： 提供了特异性结合抗原的造血祖细胞抗原和试剂，特别是抗体。 抗原的表达是高度组织特异性的。 仅在衍生自人骨髓，胎儿骨髓和肝脏，脐带血和成人外周血的造血祖细胞的子集上检测到。 由AC133识别的细胞亚群是CD34bright并且基本上包含存在于CD34 +群体中的所有CFU-GM活性。 这种高度特异性的AC133分布使其特别有用作分离和表征人类造血祖细胞和干细胞的试剂。 选择用于表达AC133抗原的细胞可以通过选择其他造血干细胞和祖细胞标记进一步纯化。

公开(公告)号：US06468794B1

公开(公告)日：2002-10-22

申请号：US09422844

申请日：1999-10-21

申请人： Nobuko Uchida ,  David W. Buck ,  Irving Weissman

发明人： Nobuko Uchida ,  David W. Buck ,  Irving Weissman

IPC分类号： C12N508

CPC分类号： C12N5/0623 ,  A61K35/12 ,  C07K16/28 ,  C07K16/2896 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2501/91

摘要： Enriched neural stem and progenitor cell populations, and methods for identifying, isolating and enriching for neural stem cells using reagent that bind to cell surface markers, are provided.

公开(公告)号：US06239587B1

公开(公告)日：2001-05-29

申请号：US09001815

申请日：1997-12-31

申请人： David W. Buck

发明人： David W. Buck

IPC分类号： G01R2726

CPC分类号： G01R1/24 ,  G01R1/06772 ,  G01R27/16

摘要： A probe (10) has a main body (12), an input side (14) and an output side (16). The main body (12) has a cavity (17) to receive an insulator (18). The insulator (18) has a current slit (22) and a voltage slit (24) aligned with a first aperture (30a) and a second aperture (30b). An inner conductor extends through the insulator (18) from the input side (14) to the output side (42) of the main body (12). A current sensor (40) inserts into the first aperture (30a) and the current slit (22). A voltage sensor (50) inserts into the second aperture (30b) and the voltage slit (24). A radio frequency signal on a transmission line having a same impedance as the probe (10) enters the inner conductor (20) at the input side (14), induces a current onto the current sensor (40) and a voltage onto the voltage sensor (50). The induced current and voltage can be measured to monitor the characteristics of the transmission line.

摘要翻译： 探针（10）具有主体（12），输入侧（14）和输出侧（16）。 主体（12）具有用于容纳绝缘体（18）的空腔（17）。 绝缘体（18）具有与第一孔（30a）和第二孔（30b）对准的电流狭缝（22）和电压狭缝（24）。 内导体从输入侧（14）延伸穿过绝缘体（18）到主体（12）的输出侧（42）。 电流传感器（40）插入到第一孔（30a）和当前狭缝（22）中。 电压传感器（50）插入第二孔（30b）和电压狭缝（24）中。 在具有与探头（10）相同阻抗的传输线上的射频信号在输入侧（14）进入内部导体（20），在电流传感器（40）上感应出电流，并将电压传递到电压传感器 （50）。 可以测量感应电流和电压以监测传输线的特性。

公开(公告)号：US06806683B2

公开(公告)日：2004-10-19

申请号：US10248631

申请日：2003-02-02

申请人： Michael R. Saldana ,  David W. Buck ,  Kenneth W. Sauter

发明人： Michael R. Saldana ,  David W. Buck ,  Kenneth W. Sauter

IPC分类号： H02J700

CPC分类号： H01M2/1022 ,  H01M2/1033 ,  H01M10/425 ,  H02M3/156 ,  H02M2001/0022

摘要： A battery adapter (A) replaces two batteries (10) with a single battery (10) housed within a compartment (48) of a battery powered electrical device (D). An electrically conductive housing (40) adapted to replicate a selected battery and fit within a space adapted to house two of the selected sized batteries. A step up circuit (18) mounted within the battery housing (40) receives an electrical signal from a single battery (10) and transforms the voltage of the electrical signal to simulate an electrical signal from two electrically connected selected batteries (10).

摘要翻译： 电池适配器（A）用容纳在电池供电的电气设备（D）的隔室（48）内的单个电池（10）代替两个电池（10）。 导电壳体（40），其适于复制选定的电池并且装配在适于容纳两个所选择的尺寸的电池的空间内。 安装在电池壳体（40）内的升压电路（18）从单个电池（10）接收电信号，并且转换电信号的电压以模拟来自两个电连接的选定电池（10）的电信号。

公开(公告)号：US5423942A

公开(公告)日：1995-06-13

申请号：US262800

申请日：1994-06-20

申请人： Roger A. Robbins ,  Donald E. Brown ,  David W. Buck ,  Alan D. Rose

发明人： Roger A. Robbins ,  Donald E. Brown ,  David W. Buck ,  Alan D. Rose

IPC分类号： H01J37/32 ,  H05H1/00

CPC分类号： H01J37/32477 ,  H01J37/32357 ,  Y10S438/935

摘要： A method and apparatus is provided for reducing wall erosion in a plasma containment tube (20), such as, for example, a quartz plasma tube (20) used in a microwave-induced plasma reaction process for etching semiconductor wafers. A pure benign or non-corrosive gas (Ar) is introduced into the "upstream" section (22a) of the tube (22), where the microwave energy is imparted to create a plasma. The activated benign gas flows "downstream" through a flange (28), preferably made of quartz, which is seated on o-rings (50) inside a water-cooled metal flange (48). These sealing o-rings (50) are thus cooled and removed from the ultraviolet light created by the plasma. The corrosive etchant gas (SF.sub.6) is introduced into the "downstream" section (22b) of the tube (22) beyond the flange (28), where it is activated by the benign gas (Ar). The benign gas (Ar) flows principally along the inner sidewalls of the tube (22), and the etchant gas (SF.sub.6) is thus principally contained by the benign gas (Ar) in the center of this section (22b) of the tube. This "downstream" section (22b) of the tube (22) is surrounded by a water jacket (24), which channels coolant around the outer surface of the tube (22b). Consequently, etching erosion of the inner surface of the tube (22) is minimized in the "upstream" or microwave section (22a) where the plasma is hottest, and also in the "downstream" section (22b) where the corrosive etchant gas (SF.sub.6) is activated.

摘要翻译： 提供了一种用于减小等离子体容纳管（20）中的壁侵蚀的方法和装置，例如在用于蚀刻半导体晶片的微波诱导等离子体反应过程中使用的石英等离子体管（20）。 将纯的良性或非腐蚀性气体（Ar）引入到管（22）的“上游”部分（22a）中，其中施加微波能量以产生等离子体。 活化的良性气体通过位于水冷金属法兰（48）内部的O形环（50）上的凸缘（28）流动，优选由石英制成。 因此，这些密封O形环（50）被冷却并从由等离子体产生的紫外线去除。 腐蚀性蚀刻剂气体（SF6）被引入超过法兰（28）的管（22）的“下游”部分（22b）中，其中它被良性气体（Ar）激活。 良性气体（Ar）主要沿着管（22）的内侧壁流动，因此蚀刻剂气体（SF6）主要由管的该部分（22b）的中心的良性气体（Ar）包含。 管（22）的“下游”部分（22b）由水套（24）包围，水套（24）围绕管（22b）的外表面通入冷却剂。 因此，在等离子体最热的“上游”或微波部分（22a）以及腐蚀性蚀刻剂气体（“...”）的“下游”部分（22b）中，管（22）的内表面的蚀刻腐蚀最小化 SF6）被激活。

公开(公告)号：US5055560A

公开(公告)日：1991-10-08

申请号：US481030

申请日：1990-02-16

申请人： David W. Buck ,  Jane M. Bindl

发明人： David W. Buck ,  Jane M. Bindl

IPC分类号： C07K16/28 ,  C07K16/30 ,  C12P21/08

CPC分类号： C07K16/28 ,  C07K16/30 ,  Y10S435/942

摘要： L60 is a novel monoclonal antibody that will react with Leu 22. It is equally reactive in formalin, ZnSO.sub.4 or Bouin's fixed, paraffin-embedded tissues as well as in frozen tissues.

摘要翻译： L60是一种能与Leu 22反应的新型单克隆抗体。它在福尔马林，ZnSO4或Bouin固定的石蜡包埋组织以及冷冻组织中同样具有反应性。

公开(公告)号：US20100086998A1

公开(公告)日：2010-04-08

申请号：US12556250

申请日：2009-09-09

申请人： David W. Buck ,  Nobuko Uchida ,  Irving Weissman

发明人： David W. Buck ,  Nobuko Uchida ,  Irving Weissman

IPC分类号： C12N5/071 ,  C12N5/02

CPC分类号： C07K16/2896 ,  A61K35/12 ,  C07K16/28 ,  C12N5/0623 ,  C12N2500/32 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2501/91 ,  G01N33/56966

摘要： Enriched neural stem and progenitor cell populations, and methods for identifying, isolating and enriching for neural stem cells using reagent that bind to cell surface markers, are provided.

摘要翻译： 提供了丰富的神经干细胞和祖细胞群，以及使用与细胞表面标记结合的试剂鉴定，分离和富集神经干细胞的方法。