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1.发明授权Methods for generating databases and databases for identifying polymorphic genetic markers 有权用于生成用于鉴定多态性遗传标记的数据库和数据库的方法公开(公告)号：US08818735B2
公开(公告)日：2014-08-26
申请号：US13536807
申请日：2012-06-28
申请人： Andreas Braun , Hubert Köster , Dirk Johannes Van Den Boom , Ping Yip , Charles Rodi , Liyan He , Norman Chiu , Christian Jurinke
发明人： Andreas Braun , Hubert Köster , Dirk Johannes Van Den Boom , Ping Yip , Charles Rodi , Liyan He , Norman Chiu , Christian Jurinke
CPC分类号： C12Q1/6872 , C12Q1/6827 , C12Q1/6883 , C12Q2600/156 , Y10T436/24 , C12Q2561/125 , C12Q2565/627 , C12Q2521/301 , C12Q2535/125
摘要： Processes and methods for creating a database of genomic samples from healthy human donors, methods that use the database to identify and correlate polymorphic genetic markers and other markers with diseases and conditions are provided.
摘要翻译： 提供了从健康人类供体创建基因组样本数据库的方法和方法，使用数据库识别多态遗传标记和其他标志物与疾病和病症相关联的方法。
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公开(公告)号：US10604791B2
公开(公告)日：2020-03-31
申请号：US13551486
申请日：2012-07-17
IPC分类号： C12Q1/68 , C12P19/34 , C12Q1/6823 , C12Q1/6809 , C12Q1/6853 , C12Q1/6858
摘要： Provided herein are products and processes for detecting the presence or absence of multiple target nucleic acids. Certain methods include amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.
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公开(公告)号：US08133701B2
公开(公告)日：2012-03-13
申请号：US11950395
申请日：2007-12-04
CPC分类号： C12Q1/686 , C12Q1/6827 , C12Q1/6872 , C12Q2537/143 , C12Q2531/113 , C12Q2565/627 , C12Q2521/319 , C12Q2525/161 , C12Q2561/101 , C12Q2545/114
摘要： The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and release labels for detection by mass spectrometry. This process is easily incorporated into a PCR amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.
摘要翻译： 本发明部分地涉及使用通过质谱法可检测的检测器寡核苷酸检测靶核酸的方法。 该方法使用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火的寡核苷酸探针，并通过质谱法释放标记进行检测。 该方法容易地并入PCR扩增测定中。 该方法还包括针对靶核酸的定量分析的实施方案。
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公开(公告)号：US20090111712A1
公开(公告)日：2009-04-30
申请号：US12133327
申请日：2008-06-04
CPC分类号： C12Q1/6823 , C12Q1/6827 , C12Q1/686 , C12Q1/6872 , C12Q1/6883 , C12Q2531/113 , C12Q2521/319 , C12Q2565/627 , C12Q2537/143 , C12Q2561/101 , C12Q2525/161 , C12Q2545/114
摘要： The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.
摘要翻译： 本发明部分地涉及使用通过质谱法可检测的检测器寡核苷酸检测靶核酸的方法。 该方法利用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火寡核苷酸探针，并通过质谱法释放标记进行检测。 该方法容易地并入聚合酶链反应（PCR）扩增测定中。 该方法还包括针对靶核酸的定量分析的实施方案。
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公开(公告)号：US08383795B2
公开(公告)日：2013-02-26
申请号：US13040056
申请日：2011-03-03
CPC分类号： C12Q1/6823 , C12Q1/6827 , C12Q1/686 , C12Q1/6872 , C12Q1/6883 , C12Q2531/113 , C12Q2521/319 , C12Q2565/627 , C12Q2537/143 , C12Q2561/101 , C12Q2525/161 , C12Q2545/114
摘要： The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.
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公开(公告)号：US07902345B2
公开(公告)日：2011-03-08
申请号：US12133327
申请日：2008-06-04
CPC分类号： C12Q1/6823 , C12Q1/6827 , C12Q1/686 , C12Q1/6872 , C12Q1/6883 , C12Q2531/113 , C12Q2521/319 , C12Q2565/627 , C12Q2537/143 , C12Q2561/101 , C12Q2525/161 , C12Q2545/114
摘要： The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.
摘要翻译： 本发明部分地涉及使用通过质谱法可检测的检测器寡核苷酸检测靶核酸的方法。 该方法利用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火寡核苷酸探针，并通过质谱法释放标记进行检测。 该方法容易地并入聚合酶链反应（PCR）扩增测定中。 该方法还包括针对靶核酸的定量分析的实施方案。
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7.发明授权Base specific cleavage of methylation-specific amplification products in combination with mass analysis 有权甲基化特异性扩增产物的碱基特异性切割与质量分析相结合公开(公告)号：US09249456B2
公开(公告)日：2016-02-02
申请号：US11089805
申请日：2005-03-24
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6858 , C12Q2565/627 , C12Q2523/125 , C12Q2525/143
摘要： Methods, combinations and kits are provided for identifying the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or for identifying the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule. Methylation state identification is performed by treating a methylated target nucleic acid molecule with a reagent that modifies one or more nucleotides in the target nucleic acid molecule as a function of the methylation state of the target nucleic acid molecule, methylation specifically amplifying treated target nucleic acid molecule, fragmenting amplified products, and detecting one or more fragments to thereby identify the methylation state of a target nucleic acid molecule, the methylation state of a nucleotide locus in a target nucleic acid molecule, or the locus of one or more methylated or unmethylated nucleotides in a target nucleic acid molecule.
摘要翻译： 提供了用于鉴定靶核酸分子的甲基化状态，靶核酸分子中的核苷酸位点的甲基化状态或鉴定靶核酸中一个或多个甲基化或非甲基化核苷酸的位点的方法，组合和试剂盒 酸分子。 通过用靶核酸分子修饰一个或多个核苷酸的试剂处理甲基化靶核酸分子，作为靶核酸分子的甲基化状态的函数，特异性扩增处理的靶核酸分子的甲基化，进行甲基化状态鉴定 ，片段扩增产物，检测一个或多个片段，从而鉴定靶核酸分子的甲基化状态，靶核酸分子中的核苷酸位点的甲基化状态或一个或多个甲基化或非甲基化核苷酸的位点 靶核酸分子。
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公开(公告)号：US08722336B2
公开(公告)日：2014-05-13
申请号：US13481612
申请日：2012-05-25
CPC分类号： C12Q1/683 , C12Q1/686 , C12Q2537/143 , C12Q2527/137 , C12Q2521/301 , C12Q2531/113
摘要： Provided in part herein is an improved method for the detection of specific polymorphic alleles in a mixed DNA population. The method comprises enriching the relative percentage of a given polymorphic allele that is exponentially amplifiable by PCR. Provided also are methods for selectively enriching target nucleic acid, for example, fetal nucleic acid in a maternal sample. In the case of detecting fetal nucleic acid in a maternal sample, a restriction enzyme is introduced that can discriminate between the alleles of a polymorphic site. In some embodiments, the maternal allele is digested and nucleic acid comprising the paternal allele is relatively enriched.
摘要翻译： 本文部分提供了用于检测混合DNA群体中特定多态性等位基因的改进方法。 该方法包括富集通过PCR指数扩增的给定多态性等位基因的相对百分比。 还提供了选择性富集靶核酸例如母体样品中的胎儿核酸的方法。 在检测母体样品中的胎儿核酸的情况下，引入可以区分多态性位点的等位基因的限制酶。 在一些实施方案中，母体等位基因被消化，并且包含父系等位基因的核酸相对富集。
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9.发明授权公开(公告)号：US08349566B2
公开(公告)日：2013-01-08
申请号：US13193390
申请日：2011-07-28
CPC分类号： C12Q1/6858 , C12Q1/6886 , C12Q2600/156 , C12Q2600/16 , C12Q2537/157 , C12Q2537/143 , C12Q2525/155
摘要： Provided herein are optimized methods for performing multiplexed detection of a plurality of sequence variations. Also provided are methods for performing multiplexed amplification of target nucleic acid.
摘要翻译： 本文提供了用于执行多个序列变化的多路复用检测的优化方法。 还提供了用于进行靶核酸的多重扩增的方法。
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公开(公告)号：US20110160093A1
公开(公告)日：2011-06-30
申请号：US13040056
申请日：2011-03-03
CPC分类号： C12Q1/6823 , C12Q1/6827 , C12Q1/686 , C12Q1/6872 , C12Q1/6883 , C12Q2531/113 , C12Q2521/319 , C12Q2565/627 , C12Q2537/143 , C12Q2561/101 , C12Q2525/161 , C12Q2545/114
摘要： The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.
摘要翻译： 本发明部分地涉及使用通过质谱法可检测的检测器寡核苷酸检测靶核酸的方法。 该方法利用核酸聚合酶的5'至3'核酸酶活性从杂交双链体切割退火寡核苷酸探针，并通过质谱法释放标记进行检测。 该方法容易地并入聚合酶链反应（PCR）扩增测定中。 该方法还包括针对靶核酸的定量分析的实施方案。
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