Process for the fermentative preparation of L-amino acids using coryneform bacteria
    1.
    发明申请
    Process for the fermentative preparation of L-amino acids using coryneform bacteria 审中-公开
    使用棒状细菌发酵制备L-氨基酸的方法

    公开(公告)号:US20050266536A1

    公开(公告)日:2005-12-01

    申请号:US11124291

    申请日:2005-05-09

    摘要: A process for the preparation of L-amino acids, in which the following steps are carried out: (a) fermentation of the coryneform bacteria which produce the desired L-amino acid and in which at least the gene which codes for trehalose phosphatase and/or the gene which codes for maltooligosyl-trehalose synthase and/or the gene which codes for maltooligosyl-trehalose trehalohydrolase is or are attenuated, (b) concentration of the desired L-amino acid in the medium or in the cells of the bacteria, and (c) isolation of the L-amino acid, and optionally bacteria in which further genes of the biosynthesis pathway of the desired L-amino acid are addtionally enhanced are employed, or bacteria in which the metabolic pathways which reduce the formation of the desired L-amino acid are at least partly eliminated are employed.

    摘要翻译: 一种制备L-氨基酸的方法,其中进行以下步骤:(a)产生所需L-氨基酸的棒状细菌的发酵,其中至少编码海藻糖磷酸酶和/ 或编码麦芽寡糖基 - 海藻糖合成酶的基因和/或编码麦芽寡糖基 - 海藻糖海藻糖水解酶的基因被减弱,(b)所需的L-氨基酸在培养基或细胞中的浓度,以及 (c)分离L-氨基酸,以及任选的细菌,其中所需L-氨基酸的生物合成途径的其它基因被附加地增强,其中代谢途径减少所需L的形成的细菌 氨基酸至少部分被消除。

    Alleles of the OpcA gene from coryneform bacteria
    4.
    发明授权
    Alleles of the OpcA gene from coryneform bacteria 有权
    来自棒状细菌的OpcA基因的等位基因

    公开(公告)号:US07700313B2

    公开(公告)日:2010-04-20

    申请号:US11383253

    申请日:2006-05-15

    IPC分类号: C12P1/00 C12P13/04

    摘要: The invention relates to mutants and alleles of the opcA gene of coryneform bacteria, which encode variants of the OpcA subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles.

    摘要翻译: 本发明涉及编码葡萄糖-6-磷酸脱氢酶(EC:1.1.1.49)的OpcA亚基变体的棒状杆菌细菌的opcA基因的突变体和等位基因,以及制备氨基酸,特别是L-赖氨酸和 L-色氨酸通过使用含有所述等位基因的细菌。

    Alleles of the GND gene from coryneform bacteria
    9.
    发明授权
    Alleles of the GND gene from coryneform bacteria 失效
    来自棒状细菌的GND基因的等位基因

    公开(公告)号:US07563602B2

    公开(公告)日:2009-07-21

    申请号:US12016728

    申请日:2008-01-18

    摘要: The invention relates to mutants and alleles of the gnd gene from coryneform bacteria coding for 6-phosphogluconate dehydrogenases which contain at position 329 or a comparable position of the amino acid sequence any amino acid other than L-valine, and to processes for the production of amino acids, preferably L-lysine and L-tryptophan, by fermentation using bacteria that contain these alleles.

    摘要翻译: 本发明涉及编码6-磷酸葡萄糖酸脱氢酶的棒状细菌的gnd基因的突变体和等位基因,其含有第329位或氨基酸序列与L-缬氨酸以外的任何氨基酸的可比较位置,以及用于生产 氨基酸,优选L-赖氨酸和L-色氨酸,通过使用含有这些等位基因的细菌进行发酵。