公开(公告)号：US5403728A

公开(公告)日：1995-04-04

申请号：US77364

申请日：1993-06-17

申请人： Antonia Jekkel ,  Eva Ilkoy ,  Istvan M. Szabo ,  Gabor Ambrus ,  Attila Andor ,  Ilona Varga ,  Imre Moravcsik ,  Istvan Szabo ,  Janos Erdei ,  Kalman Polya ,  Andras Kiss ,  Laszlo Cseke ,  Karoly Nagy ,  Mihaly Kaszas ,  Lajos Kiss ,  Istvan Magyi ,  Edit Halasz ,  Gyorgy Santha

发明人： Antonia Jekkel ,  Eva Ilkoy ,  Istvan M. Szabo ,  Gabor Ambrus ,  Attila Andor ,  Ilona Varga ,  Imre Moravcsik ,  Istvan Szabo ,  Janos Erdei ,  Kalman Polya ,  Andras Kiss ,  Laszlo Cseke ,  Karoly Nagy ,  Mihaly Kaszas ,  Lajos Kiss ,  Istvan Magyi ,  Edit Halasz ,  Gyorgy Santha

IPC分类号： C07D309/30 ,  C12P7/62 ,  C12P17/06 ,  C12P7/00 ,  C12P7/02

CPC分类号： C12R1/66 ,  C07D309/30 ,  C12P17/06 ,  C12P7/62

摘要： The invention refers to a new microbial process for preparing .beta.,.delta.-dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methyl-butyryloxy)-naphthalen-1-yl]-heptanoic acid .delta.-lactone of formula (I) and .beta.,.delta.-dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methylbutyryloxy)-naphtalen-1-yl]-heptanoic acid of formula (II), ##STR1## by the aerobic fermentation of the submerged culture of an imperfect fungus strain biosynthesizing the above compounds, in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product of formula (I), which comprises cultivating a strain of the new Aspergillus obscurus fungus species producing the above compound(s) of formulas (I) and/or (II), in a temperature range of 25.degree. to 30.degree. C. and, if desired, separating the product(s) formed from the fermentation broth, then isolating it in the lactone form of formula (I) and, if desired, purifying the same.

摘要翻译： 本发明涉及一种制备β，δ-二羟基-7- [1,2,6,7,8,8a-六氢-2,6-二甲基-8-（2-甲基 - 丁酰氧基） - 萘-1 - 基] - 庚酸δ-内酯和β，二羟基-7- [1,2,6,7,8,8a-六氢-2,6-二甲基-8-（2-甲基丁酰氧基） - 萘醌-1-基] - 庚酸，通过将生物合成上述化合物的不完全真菌菌株的浸没培养物的需氧发酵在含有（II）的营养培养基中的含氧培养基中， 可利用的碳源和氮源以及矿物盐，并且通过分离式（I）的产物，其包括培养产生上述式（I）和/或（I）的化合物的新的黑曲霉菌种的菌株， II），在25℃至30℃的温度范围内，如果需要，分离由发酵液形成的产物，然后以式（I）的内酯形式分离，如果需要，将其纯化 一样。

公开(公告)号：US5156960A

公开(公告)日：1992-10-20

申请号：US454005

申请日：1989-12-20

申请人： Antonia Jekkel nee Bokany ,  Gabor Ambrus ,  Eva Toth-Sarudy ,  Istvan Mihaly ,  Agota Hulber nee Dobos ,  Attila Andor ,  Karoly Albrecht ,  Kalman Konczol ,  Valeria Szell ,  Eva Tomori nee Joszt ,  Imre Moravcsik ,  Kalman Polya ,  Janos Erdei ,  Lajos Kiss ,  Bela Makadi ,  Karoly Nagy ,  Bela Palotas ,  Etelka Deli nee Konszky ,  Karoly Buzasi ,  Aniko Molnar nee Antal ,  Gyorgy Santha ,  Vilma Szaszhegyesi

发明人： Antonia Jekkel nee Bokany ,  Gabor Ambrus ,  Eva Toth-Sarudy ,  Istvan Mihaly ,  Agota Hulber nee Dobos ,  Attila Andor ,  Karoly Albrecht ,  Kalman Konczol ,  Valeria Szell ,  Eva Tomori nee Joszt ,  Imre Moravcsik ,  Kalman Polya ,  Janos Erdei ,  Lajos Kiss ,  Bela Makadi ,  Karoly Nagy ,  Bela Palotas ,  Etelka Deli nee Konszky ,  Karoly Buzasi ,  Aniko Molnar nee Antal ,  Gyorgy Santha ,  Vilma Szaszhegyesi

IPC分类号： C12N1/14 ,  A61K31/545 ,  A61K38/00 ,  A61P37/06 ,  C07D501/00 ,  C07K7/64 ,  C12P1/00 ,  C12P21/04 ,  C12P35/00 ,  C12R1/645

CPC分类号： C12R1/645 ,  C07K7/645 ,  Y10S435/911

摘要： The invention relates to a microbial process for the production of immunosuppressive cyclosporine antibiotic complex or of its components, cyclosporine A, cyclosporine B and cyclosporine C, by the aerobic fermentation of a filamentous fungus strain biosynthesizing the above antibiotic(s) in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the products formed, which comprises culturing a strain of the novel Tolypocladium varium fungus species producing the cyclosporine antibiotic complex, preferably tolypocladium varium sp. nov. CY/93, deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM(P)F 001005, on a nutrient medium containing carbon sources, organic and inorganic nitrogen sources as well as mineral salts, under aerobic conditions, at 25.degree. to 30.degree. C., and, if desired, isolating and purifying the cyclosporine antibiotic complex or its components produced.

摘要翻译： 本发明涉及通过在含营养培养基中生物合成上述抗生素的丝状真菌菌株的需氧发酵来生产免疫抑制环孢菌素抗生素复合物或其组分的环孢菌素A，环孢菌素B和环孢霉素C的微生物方法， 可利用的碳源和氮源以及矿物盐，并且通过分离形成的产物，其包括培养产生环孢菌素抗生素复合物的新型Tolypocladium v​​arium真菌物种的菌株，优选地变种球菌sp。 nov。 CY / 93，保藏在匈牙利布达佩斯国家农业和工业微生物收集库，编号为NCAIM（P）F 001005），在有氧条件下，含有碳源，有机和无机氮源以及矿物盐的营养培养基 ，25℃至30℃，如果需要，分离和纯化产生的环孢菌素抗生素复合物或其组分。

公开(公告)号：US06696599B2

公开(公告)日：2004-02-24

申请号：US10437058

申请日：2003-05-14

申请人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Mozes Nee Suto ,  Janso Salat ,  Attila Andor ,  Laszlo Birincsik ,  Sandor Boros ,  Ildiko Lang ,  Margit Bidlo Nee Igloy

发明人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Mozes Nee Suto ,  Janso Salat ,  Attila Andor ,  Laszlo Birincsik ,  Sandor Boros ,  Ildiko Lang ,  Margit Bidlo Nee Igloy

IPC分类号： C07C6702

CPC分类号： C12R1/645 ,  C12P7/62

摘要： The present invention relates to a new microbial process for the preparation of the compound formula (I) from a compound of general formula (II) wherein R stands for an alkali metal or ammonium ion, by the submerged cultivation of a mold strain able to 6&bgr;-hydroxylate a compound of the Formula (II) in aerobic fermentation and by the separation and purification of the product of Formula (I) formed in the course of the bioconversion. The process comprises cultivating a strain of Mortierella maculata filamentous mold species that is able to 6&bgr;-hydroxylate a compound of the general Formula (II), on a nutrient medium containing assimilable carbon and nitrogen sources and mineral salts and separating the product formed from the fermentation broth, then isolating the compound of formula (I) and purifying the same. Novel strains of Mortierella maculata are also disclosed.

摘要翻译： 本发明涉及一种由通式（II）的化合物制备式（I）化合物的新的微生物方法，其中R代表碱金属或铵离子，通过浸没式培养能够提供6β 在需氧发酵中通过分解和纯化在生物转化过程中形成的式（I）的产物来氢化式（II）化合物。 该方法包括在含有可吸收的碳和氮源和矿物盐的营养培养基上培养能够将通式（II）的化合物6β-羟基化的高山被孢霉丝状菌种的菌株，并分离由发酵形成的产物 肉汤，然后分离式（I）化合物并纯化。 还公开了高山被孢霉的新型菌株。

公开(公告)号：US20060281155A1

公开(公告)日：2006-12-14

申请号：US11509662

申请日：2006-08-25

申请人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Suto ,  Janos Salat ,  Attila Andor ,  Laszlo Birincsik ,  Sandor Boros ,  Ildiko Lang ,  Margit Nee Igloy

发明人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Suto ,  Janos Salat ,  Attila Andor ,  Laszlo Birincsik ,  Sandor Boros ,  Ildiko Lang ,  Margit Nee Igloy

IPC分类号： C12P7/62

CPC分类号： C12R1/645 ,  C12P7/62

摘要： The present invention relates to a new microbial process for the preparation of the compound formula (I) from a compound of general formula (II) wherein R stands for an alkali metal or ammonium ion, by the submerged cultivation of a mold strain able to 6β-hydroxylate a compound of the Formula (II) in aerobic fermentation and by the separation and purification of the product of Formula (I) formed in the course of the bioconversion. The process comprises cultivating a strain of Mortierella maculata filamentous mold species that is able to 6β-hydroxylate a compound of the general Formula (II), on a nutrient medium containing assimilable carbon and nitrogen sources and mineral salts and separating the product formed from the fermentation broth, then isolating the compound of formula (I) and purifying the same. Novel strains of Mortierella maculata are also disclosed.

公开(公告)号：US06750366B2

公开(公告)日：2004-06-15

申请号：US10011176

申请日：2001-12-05

申请人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Mozes Nee Suto ,  Janso Salat ,  Attila Andor ,  Laszlo Birinesik ,  Sandor Boros ,  Ildiko Lang ,  Margit Bidlo Nee Igloy

发明人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Mozes Nee Suto ,  Janso Salat ,  Attila Andor ,  Laszlo Birinesik ,  Sandor Boros ,  Ildiko Lang ,  Margit Bidlo Nee Igloy

IPC分类号： C07C6902

CPC分类号： C12R1/645 ,  C12P7/62

摘要： The present invention relates to a new microbial process for the preparation of the compound formula (I): from a compound of general formula (II): wherein R stands for an alkali metal or ammonium ion, by the submerged cultivation of a mold strain able to 6&bgr;-hydroxylate a compound of the Formula (II) in aerobic fermentation and by the separation and purification of the product of Formula (I) formed in the course of the bioconversion. The process comprises cultivating a strain of Mortierella maculata filamentous mold species that is able to 6&bgr;-hydroxylate a compound of the general Formula (II), on a nutrient medium containing assimilable carbon and nitrogen sources and mineral salts and separating the product formed from the fermentation broth, then isolating the compound of formula (I) and purifying the same. Novel strains of Mortierella maculata are also disclosed.

摘要翻译： 本发明涉及一种制备式（I）化合物的新的微生物方法：由通式（II）的化合物：其中R代表碱金属或铵离子，通过浸没式培养可变形的模具 在有氧发酵中通过分解和纯化在生物转化过程中形成的式（I）的产物来将式（II）的化合物改性为6β-羟基化。 该方法包括在含有可吸收的碳和氮源和矿物盐的营养培养基上培养能够将通式（II）的化合物6β-羟基化的高山被孢霉丝状菌种的菌株，并分离由发酵形成的产物 肉汤，然后分离式（I）化合物并纯化。 还公开了高山被孢霉的新型菌株。

公开(公告)号：US06682913B1

公开(公告)日：2004-01-27

申请号：US09497805

申请日：2000-02-03

申请人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Mozes nee Suto ,  Janso Salat ,  Attila Andor ,  Laszlo Birincsik ,  Sandor Boros ,  Ildiko Lang ,  Margit Bidlo nee Igloy

发明人： Antonia Jekkel ,  Attila Konya ,  Istvan Barta ,  Eva Ilkoy ,  Gyorgy Somogyi ,  Gabor Ambrus ,  Gyula Horvath ,  Karoly Albrecht ,  Istvan Szabo ,  Julianna Mozes nee Suto ,  Janso Salat ,  Attila Andor ,  Laszlo Birincsik ,  Sandor Boros ,  Ildiko Lang ,  Margit Bidlo nee Igloy

IPC分类号： C12P762

CPC分类号： C12R1/645 ,  C12P7/62

摘要： The present invention relates to a new microbial process for the preparation of the compound formula (I) from a compound of general formula (II) wherein R stands for an alkali metal or ammonium ion, by the submerged cultivation of a mold strain able to 6&bgr;-hydroxylate a compound of the Formula (II) in aerobic fermentation and by the separation and purification of the product of Formula (I) formed in the course of the bioconversion. The process comprises cultivating a strain of Mortierella maculata filamentous mold species that is able to 6&bgr;-hydroxylate a compound of the general Formula (II), on a nutrient medium containing assimilable carbon and nitrogen sources and mineral salts and separating the product formed from the fermentation broth, then isolating the compound of formula (I) and purifying the same. Novel strains of Mortierella maculata are also disclosed.

公开(公告)号：US06812007B1

公开(公告)日：2004-11-02

申请号：US09578587

申请日：2000-04-19

申请人： Vilmos Kéri ,  Irma Högye ,  Antónia Jekkel ,  Ilona Bagdi ,  Gábor Ambrus ,  Attila Jakab ,  Attila Andor ,  Lajos Deák ,  István Szabó ,  János Bálint ,  Zsuzsanna Scheidl ,  Etelka Deli ,  Gyula Horváth ,  Csaba Szabó ,  Ildikó Láng ,  Imre Székely ,  Imre Moravcsik ,  Vera Kovács ,  Szabolcs Mátyás ,  Zsuzsanna Sztáray ,  László Eszenyi ,  Éva Ilköy

发明人： Vilmos Kéri ,  Irma Högye ,  Antónia Jekkel ,  Ilona Bagdi ,  Gábor Ambrus ,  Attila Jakab ,  Attila Andor ,  Lajos Deák ,  István Szabó ,  János Bálint ,  Zsuzsanna Scheidl ,  Etelka Deli ,  Gyula Horváth ,  Csaba Szabó ,  Ildikó Láng ,  Imre Székely ,  Imre Moravcsik ,  Vera Kovács ,  Szabolcs Mátyás ,  Zsuzsanna Sztáray ,  László Eszenyi ,  Éva Ilköy

IPC分类号： C12P1706

CPC分类号： C12P17/06 ,  C12P7/62

摘要： In a process for preparing mevinolin by fermentation of a biomass in a fermentation liquor, which includes dissolving mevinolin from the biomass into the fermentation liquor, and separating the biomass from the fermentation liquor to obtain a separated fermentation liquor, separating the mevinolin from the separated fermentation liquor, and recovering the end product, the improvement which comprises carrying out the dissolving at a pH between 7.5 and about 10, and the separating of the mevinolin is carried out at a pH between about 4.5 and about 1.

摘要翻译： 在发酵液中通过发酵生物质来制备美维林的方法，其包括将生物质中的美维林溶解于发酵液中，并将生物质与发酵液分离以获得分离的发酵液，从分离的发酵液中分离出美维林 液，并回收最终产物，其改进包括在7.5至约10之间的pH下进行溶解，并且在约4.5至约1之间的pH下进行甲维林的分离。

公开(公告)号：US08685690B2

公开(公告)日：2014-04-01

申请号：US13416608

申请日：2012-03-09

申请人： Jie Yang ,  Xiyun Zhang ,  Attila Andor

发明人： Jie Yang ,  Xiyun Zhang ,  Attila Andor

IPC分类号： C12N9/42 ,  C12P19/02 ,  C12P19/00

CPC分类号： C12N9/42 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  Y02E50/16

摘要： The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

摘要翻译： 本发明涉及真菌C1菌株和葡萄糖苷酶的变体形式的重组表达。 本发明还涉及通过使用表达“葡糖苷酶”变体的转基因生物体从生物质产生可发酵糖并通过发酵糖生产生物燃料。 本发明提供了通过将纤维二糖与重组体 - 葡糖苷酶变体蛋白（例如由重组宿主细胞分泌的变体蛋白）接触到培养基中来从纤维二糖生产可发酵糖例如葡萄糖的方法。 本发明的方法可用于将生物质底物转化为可发酵的糖，最终转化为乙醇或其他生物燃料。

公开(公告)号：US08323947B2

公开(公告)日：2012-12-04

申请号：US13416555

申请日：2012-03-09

申请人： Jie Yang ,  Xiyun Zhang ,  Attila Andor

发明人： Jie Yang ,  Xiyun Zhang ,  Attila Andor

IPC分类号： C12N9/42 ,  C12N15/00 ,  C12N1/00 ,  C12P19/02 ,  C12P19/00 ,  C12P21/06

CPC分类号： C12N9/42 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  Y02E50/16

摘要： The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

公开(公告)号：US08143050B2

公开(公告)日：2012-03-27

申请号：US12954447

申请日：2010-11-24

申请人： Jie Yang ,  Xiyun Zhang ,  Attila Andor

发明人： Jie Yang ,  Xiyun Zhang ,  Attila Andor

IPC分类号： C12N9/42 ,  C12P19/02 ,  C12P19/00 ,  C07H21/04

CPC分类号： C12N9/42 ,  C12N9/2434 ,  C12N9/2445 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  Y02E50/16

摘要： The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.

摘要翻译： 本发明涉及真菌C1菌株和葡萄糖苷酶的变体形式的重组表达。 本发明还涉及通过使用表达“葡糖苷酶”变体的转基因生物体从生物质产生可发酵糖并通过发酵糖生产生物燃料。 本发明提供了通过将纤维二糖与重组体 - 葡糖苷酶变体蛋白（例如由重组宿主细胞分泌的变体蛋白）接触到培养基中来从纤维二糖生产可发酵糖例如葡萄糖的方法。 本发明的方法可用于将生物质底物转化为可发酵的糖，最终转化为乙醇或其他生物燃料。