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    Production and secretion of proteins of bacterial origin in filamentous fungi
    1.
    发明授权
    Production and secretion of proteins of bacterial origin in filamentous fungi 有权
    细菌起源于丝状真菌的蛋白质的生产和分泌

    公开(公告)号：US07816129B2

    公开(公告)日：2010-10-19

    申请号：US10286993

    申请日：2002-08-13

    申请人： Arja Mäntylä ,  Marja Paloheimo ,  Raija Lantto ,  Richard Fagerström ,  Tarja Lahtinen ,  Pirkko Suominen ,  Jari Vehmaanperä

    发明人： Arja Mäntylä ,  Marja Paloheimo ,  Raija Lantto ,  Richard Fagerström ,  Tarja Lahtinen ,  Pirkko Suominen ,  Jari Vehmaanperä

    IPC分类号： C12N15/00 ,  C12N9/00 ,  C12N9/24 ,  C12N9/30 ,  C07H21/04

    CPC分类号： C12Y302/01032 ,  C12N9/2434 ,  C12N9/248 ,  C12N15/80 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/01004 ,  C12Y302/01006 ,  C12Y302/01008 ,  C12Y302/01025 ,  C12Y302/01039 ,  C12Y302/01091 ,  D21C5/005

    摘要： The present invention is related to an improved production of bacterial proteins in filamentous fungus, e.g. in Trichoderma and Aspergillus. The improvement is achieved by constructing expression vectors, which comprise the bacterial protein encoding DNA sequences fused in frame with a DNA sequence encoding a filamentous fungus secretable protein or one or more functional domains of said protein. Filamentous fungus hosts transformed with such expression vectors secrete the desired proteins or enzymes, especially xylanases or cellulases originating from bacteria or more preferably from actinornycetes into the culture medium of the host. The desired proteins or enzymes can be used directly from the culture medium after separation of host cells or recovered and treated using down-stream processes, which are appropriate for the respective application. Xylanases or cellulases from actinomycetes produced using the above expression vectors are most suitable for treating plant derived materials e.g. in pulp and paper industries.

    摘要翻译： 本发明涉及丝状真菌中细菌蛋白质的改进生产，例如， 在木霉属和曲霉属中。 通过构建表达载体来实现改善，所述表达载体包含编码与编码丝状真菌可分泌蛋白质的DNA序列或所述蛋白质的一个或多个功能结构域框架融合的DNA序列的细菌蛋白质。 用这种表达载体转化的丝状真菌宿主分泌所需的蛋白质或酶，特别是源自细菌的木聚糖酶或纤维素酶，或更优选从放线菌素分泌到宿主的培养基中。 所需的蛋白质或酶可以在分离宿主细胞之后直接从培养基中使用，或者使用适合于各自应用的下游过程进行回收和处理。 使用上述表达载体产生的放线菌的木聚糖酶或纤维素酶最适合于处理植物来源的材料，例如 在纸浆和造纸工业。