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    Purified yeast ubiquitin hydrolase
    5.
    发明授权
    Purified yeast ubiquitin hydrolase 失效
    纯化酵母泛素水解酶

    公开(公告)号：US5156968A

    公开(公告)日：1992-10-20

    申请号：US210909

    申请日：1988-06-24

    申请人： Chung-Cheng Liu

    发明人： Chung-Cheng Liu

    IPC分类号： C07K14/00 ,  C07K14/64 ,  C12N9/60 ,  C12N15/57

    CPC分类号： C12N9/60 ,  C07K14/00 ,  C07K14/64 ,  C07K2319/00 ,  C07K2319/036 ,  C07K2319/75 ,  C07K2319/95

    摘要： Ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition, which hydrolase hydrolyzes a ubiquitin-polypeptide conjugate at the amide bond linking the ubiquitin and polypeptide, thereby yielding intact polypeptide with an unconjugated, mature N-terminus. Also provided are DNA sequences encoding such ubiquitin hydrolase, as well as expression systems for its recombinant production. Processes are provided for purification of ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.

    摘要翻译： 提供泛素水解酶，其纯度基于组合物中总蛋白质的重量至少70％的均匀性，该水解酶在连接泛素和多肽的酰胺键处水解泛素 - 多肽缀合物，由此产生具有未缀合的完整多肽 ，成熟N末端。 还提供了编码这种泛素水解酶的DNA序列，以及用于其重组生产的表达系统。 提供了用于从真核生物纯化泛素水解酶的方法，并且其用于在其N-末端用泛素回收任何无融合物的所需多肽。

    Host transformed with yeast gene and ubiquitin/polypeptide fusions
    8.
    发明授权
    Host transformed with yeast gene and ubiquitin/polypeptide fusions 失效
    宿主用酵母基因和泛素/多肽融合物转化

    公开(公告)号：US5262322A

    公开(公告)日：1993-11-16

    申请号：US694522

    申请日：1991-04-30

    申请人： Chung-Cheng Liu ,  Harvey I. Miller

    发明人： Chung-Cheng Liu ,  Harvey I. Miller

    IPC分类号： C07K14/00 ,  C07K14/64 ,  C12N9/60 ,  C12N15/57 ,  C12N1/21

    CPC分类号： C07K14/00 ,  C07K14/64 ,  C12N9/60 ,  C07K2319/036 ,  C07K2319/75 ,  C07K2319/95

    摘要： A ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition. Also provided are DNA sequences encoding ubiquitin hydrolases, as well as expression systems for their recombinant production. Processes are provided for purification of a ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.

    摘要翻译： 提供泛蛋白水解酶，其基于组合物中总蛋白质的重量具有至少70％的纯度至少70％的纯度。 还提供了编码泛素水解酶的DNA序列，以及用于其重组生产的表达系统。 提供了从真核生物纯化泛素水解酶的方法，并且其用于在其N-末端用泛素回收任何无融合物的所需多肽。