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公开(公告)号：US20100113575A1
公开(公告)日：2010-05-06
申请号：US12593342
申请日：2008-03-21
IPC分类号： A61K31/7088 , C12Q1/68 , C12N9/48 , G01N33/53 , A61P35/00
CPC分类号： C12Q1/37 , C12N9/6424
摘要： The present invention provides a method for detecting autoprocessed, secreted PCSK9, a protein involved in cholesterol homeostasis, and for effectively identifying compounds that inhibit autocleavage and secretion from cells. The disclosed method involves the insertion of an epitope tag into a PCSK9 expression construct immediately C-terminal to the pro domain ending at an amino acid residue corresponding to Q152 of human PCSK9. Upon autoprocessing, the epitope tag is exposed and capable of recognition by anti-epitope antibodies or other suitable identification system, allowing for the selective and exclusive identification and/or quantification of processed PCSK9. The present disclosure thus advances the goal of providing enabling technology to the art for the effective identification of therapeutics effective in combating coronary heart disease.
摘要翻译： 本发明提供一种用于检测自身加工的分泌的PCSK9，参与胆固醇体内平衡的蛋白质以及有效地鉴定抑制细胞自噬和分泌的化合物的方法。 所公开的方法涉及将表位标签插入到终止于对应于人PCSK9的Q152的氨基酸残基的pro结构域的C末端的PCSK9表达构建体中。 在自动加工中，表位标签被暴露并且能够通过抗表位抗体或其他合适的识别系统识别，允许对加工的PCSK9的选择性和排他性鉴定和/或定量。 因此，本公开内容提供了为本领域提供有用的技术以有效鉴定有效对抗冠心病的治疗剂的目标。
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公开(公告)号：US08263353B2
公开(公告)日：2012-09-11
申请号：US12593342
申请日：2008-03-21
IPC分类号： G01N33/573 , G01N33/567
CPC分类号： C12Q1/37 , C12N9/6424
摘要： The present invention provides a method for detecting autoprocessed, secreted PCSK9, a protein involved in cholesterol homeostasis, and for effectively identifying compounds that inhibit autocleavage and secretion from cells. The disclosed method involves the insertion of an epitope tag into a PCSK9 expression construct immediately C-terminal to the pro domain ending at an amino acid residue corresponding to Q152 of human PCSK9. Upon autoprocessing, the epitope tag is exposed and capable of recognition by anti-epitope antibodies or other suitable identification system, allowing for the selective and exclusive identification and/or quantification of processed PCSK9. The present disclosure thus advances the goal of providing enabling technology to the art for the effective identification of therapeutics effective in combating coronary heart disease.
摘要翻译： 本发明提供一种用于检测自身加工的分泌的PCSK9，参与胆固醇体内平衡的蛋白质以及有效地鉴定抑制细胞自噬和分泌的化合物的方法。 所公开的方法涉及将表位标签插入到终止于对应于人PCSK9的Q152的氨基酸残基的pro结构域的C末端的PCSK9表达构建体中。 在自动加工中，表位标签被暴露并且能够通过抗表位抗体或其他合适的识别系统识别，允许对加工的PCSK9的选择性和排他性鉴定和/或定量。 因此，本公开内容提供了为本领域提供有用的技术以有效鉴定有效对抗冠心病的治疗剂的目标。
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公开(公告)号：US20120082680A1
公开(公告)日：2012-04-05
申请号：US13242831
申请日：2011-09-23
申请人： Ayesha Sitlani , Carl P. Sparrow , Shilpa Pandit , Jon H. Condra , Dana D. Wood , Timothy S. Fisher
发明人： Ayesha Sitlani , Carl P. Sparrow , Shilpa Pandit , Jon H. Condra , Dana D. Wood , Timothy S. Fisher
IPC分类号： A61K39/395 , C12N15/13 , A61P7/00 , A61P9/00 , G01N21/64 , C12N15/63 , C12N5/10 , C12N1/21 , C12N1/19 , C12Q1/02 , C07K16/40 , A61P3/00
CPC分类号： C07K16/40 , C07K2317/55 , C07K2317/56 , C07K2317/76 , C07K2317/92
摘要： Antagonists of human proprotein convertase subtilisin-kexin type 9 (“PCSK9”) are disclosed. The disclosed antagonists are effective in the inhibition of PCSK9 function and, accordingly, present desirable antagonists for the use in the treatment of conditions associated with PCSK9 activity. The present invention also discloses nucleic acid encoding said antagonists, vectors, host cells, and compositions comprising the antagonists. Methods of making PCSK9-specific antagonists as well as methods of using the antagonists for inhibiting or antagonizing PCSK9 function are also disclosed and form important additional aspects of the present disclosure.
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公开(公告)号：US20100040610A1
公开(公告)日：2010-02-18
申请号：US12312383
申请日：2007-11-02
申请人： Ayesha Sitlani , Carl P. Sparrow , Shilpa Pandit , Jon H. Condra , Dana D. Wood , Timothy S. Fisher
发明人： Ayesha Sitlani , Carl P. Sparrow , Shilpa Pandit , Jon H. Condra , Dana D. Wood , Timothy S. Fisher
IPC分类号： A61K39/395 , C07K16/18 , C07H21/00 , C12N15/74 , C12N5/07 , C12P21/02 , G01N33/53 , A61P35/00
CPC分类号： C07K16/40 , C07K2317/55 , C07K2317/56 , C07K2317/76 , C07K2317/92
摘要： Antagonists of human proprotein convertase subtilisin-kexin type 9 (“PCSK9”) are disclosed. The disclosed antagonists are effective in the inhibition of PCSK9 function and, accordingly, present desirable antagonists for the use in the treatment of conditions associated with PCSK9 activity. The present invention also discloses nucleic acid encoding said antagonists, vectors, host cells, and compositions comprising the antagonists. Methods of making PCSK9-specific antagonists as well as methods of using the antagonists for inhibiting or antagonizing PCSK9 function are also disclosed and form important additional aspects of the present disclosure.
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公开(公告)号：US20130056145A1
公开(公告)日：2013-03-07
申请号：US13526427
申请日：2012-06-18
CPC分类号： H01L23/373 , H01L2924/0002 , H01L2924/00
摘要： A method to bond carbon nanotubes to a surface. The mechanism of this bonding is studied, and shows that intercalation of alkali ions is possibly the central mechanism. Bonding pull-off forces of 4-5 N/cm2 were measured. This bonding also provides improved interfacial properties for other phenomenon, including improved thermal conductivity.
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公开(公告)号：US20120276327A1
公开(公告)日：2012-11-01
申请号：US13466259
申请日：2012-05-08
CPC分类号： H01L51/102 , B01J23/745 , B01J37/0238 , B01J37/0244 , B82Y10/00 , B82Y30/00 , H01L51/0048 , H01L2924/0002 , Y10T428/13 , Y10T428/24174 , Y10T428/24372 , Y10T428/256 , Y10T428/257 , H01L2924/00
摘要： Vertically oriented carbon nanotubes (CNT) arrays have been simultaneously synthesized at relatively low growth temperatures (i.e.,
摘要翻译： 通过等离子体增强化学气相沉积在铝箔两侧的相对低的生长温度（即<700℃）下同时合成垂直取向的碳纳米管（CNT）阵列。 得到的CNT阵列是高度致密的，并且阵列中的平均CNT直径约为10nm，由铝箔两侧直接并同时合成的CNT阵列组成的CNT CNT被制造出来。 TIM是可插入的，并且允许温度敏感和/或粗糙的衬底由高导电性和适应性的CNT阵列连接。 金属箔的使用是经济的，并且由于其广泛的应用可能在制造中证明是有利的。
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公开(公告)号：US20100200208A1
公开(公告)日：2010-08-12
申请号：US12688517
申请日：2010-01-15
CPC分类号： B01J23/745 , B01J37/0238 , B01J37/0244 , B82Y10/00 , B82Y30/00 , B82Y40/00 , C01B31/0226 , C01B31/0233 , C01B32/16 , C01B32/162 , C01B2202/08 , H01L51/0048 , H01L51/102 , H01M4/8605 , H01M4/8807 , Y10T29/49826 , Y10T428/23943
摘要： Vertically oriented carbon nanotubes (CNT) arrays have been simultaneously synthesized at relatively low growth temperatures (i.e.,
摘要翻译： 通过等离子体增强化学气相沉积在铝箔两侧的相对低的生长温度（即<700℃）下同时合成垂直取向的碳纳米管（CNT）阵列。 所得到的CNT阵列是高度致密的，并且阵列中的平均CNT直径约为10nm，已经制造了由各种类型的箔的两面直接并同时合成的CNT阵列组成的A CNT TIM。 TIM是可插入的，并且允许温度敏感和/或粗糙的衬底由高导电性和适应性的CNT阵列连接。 公开了使用箔，包括由碳（CNT编织阵列，剥离石墨片，巴氏纸等）制成的基底。
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公开(公告)号：US20080236804A1
公开(公告)日：2008-10-02
申请号：US11873952
申请日：2007-10-17
CPC分类号： H01L51/102 , B01J23/745 , B01J37/0238 , B01J37/0244 , B82Y10/00 , B82Y30/00 , H01L51/0048 , H01L2924/0002 , Y10T428/13 , Y10T428/24174 , Y10T428/24372 , Y10T428/256 , Y10T428/257 , H01L2924/00
摘要： Vertically oriented carbon nanotubes (CNT) arrays have been simultaneously synthesized at relatively low growth temperatures (i.e.,
摘要翻译： 通过等离子体增强化学气相沉积在铝箔两侧的相对低的生长温度（即<700℃）下同时合成垂直取向的碳纳米管（CNT）阵列。 得到的CNT阵列是高度致密的，并且阵列中的平均CNT直径约为10nm，由铝箔两侧直接并同时合成的CNT阵列组成的CNT CNT被制造出来。 TIM是可插入的，并且允许温度敏感和/或粗糙的衬底由高导电性和适应性的CNT阵列连接。 金属箔的使用是经济的，并且由于其广泛的应用可能在制造中证明是有利的。
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公开(公告)号：US5179501A
公开(公告)日：1993-01-12
申请号：US840247
申请日：1992-02-24
CPC分类号： H05K1/0278 , H05K3/0061 , H05K5/003 , H05K1/189 , H05K2201/0338 , H05K2201/09109 , H05K2201/10189 , H05K2203/302
摘要： An electronic module assembly includes a relatively thin, bendable base plate having at least one bend axis along which the base plate is bent over on itself to provide an enclosure. An inner surface of the base plate supports at least one, and preferably two, relatively thicker metal plates, one on each side of the bend axis, each metal plate acting as a heatsink for circuit components mounted thereon.
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公开(公告)号：US09487877B2
公开(公告)日：2016-11-08
申请号：US12024635
申请日：2008-02-01
CPC分类号： C25D7/00 , B82Y10/00 , B82Y30/00 , B82Y40/00 , C01B32/162 , C01B32/168 , C01B2202/02 , C01B2202/08 , C25D5/02 , C25D5/18 , C25D11/045
摘要： In one embodiment, SWNTs are synthesized from an embedded catalyst in a modified porous anodic alumina (PAA) template. Pd is electrodeposited into the template to form nanowires that grow from an underlying conductive layer beneath the PAA and extend to the initiation sites of the SWNTs within each pore. Individual vertical channels of SWNTs are created, each with a vertical Pd nanowire back contact. Further Pd deposition results in annular Pd nanoparticles that form on portions of SWNTs extending onto the PAA surface. Two-terminal electrical characteristics produce linear I-V relationships, indicating ohmic contact in the devices.
摘要翻译： 在一个实施方案中，SWNT由改性多孔阳极氧化铝（PAA）模板中的嵌入式催化剂合成。 将Pd电沉积到模板中以形成从PAA下面的下面的导电层生长并延伸到每个孔内的SWNT的起始位点的纳米线。 创建SWNT的单个垂直通道，每个垂直通道具有垂直的Pd纳米线背接触。 进一步的Pd沉积导致在延伸到PAA表面上的SWNT的部分上形成环状Pd纳米颗粒。 两端电特性产生线性I-V关系，表示器件中的欧姆接触。
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