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公开(公告)号:US20180073047A1
公开(公告)日:2018-03-15
申请号:US15559962
申请日:2016-02-19
Applicant: BASF SE
Inventor: Vaidotas NAVICKAS , Michael BREUER , Michael PUHL , Melanie WEINGARTEN , Kai-Uwe BALDENIUS , Wofgang SIEGEL
CPC classification number: C12P19/02 , C12N9/0006 , C12N9/0065 , C12Y101/03009 , C12Y111/01006 , C12Y111/01007
Abstract: The present invention relates to biocatalytic methods, comprising purely enzymatic, mixed enzymatic-fermentative and purely fermentative methods, for the direct single-step conversion of L-fucitol to L-fucose, in order to easily obtain L-fucose at high amounts and levels of purity. Suitable recombinant microorganisms and fungi are further disclosed and also the use thereof in said method for the single-step conversion to L-fucose.
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公开(公告)号:US20170260519A1
公开(公告)日:2017-09-14
申请号:US15512792
申请日:2015-09-30
Applicant: BASF SE
Inventor: Kerstin HAGE , Carsten SCHWALB , Petra SPROETE , Peter OEDMAN , Kai-Uwe BALDENIUS , Burkhard ERNST , Gunter STEIGELMANN , Stephan FREYER , Claus BOLLSCHWEILER , Michael Guenter BRAUN , Juergen DAEUWEL
CPC classification number: C12N9/88 , C12N1/20 , C12N1/38 , C12Y402/01084
Abstract: The present invention relates to methods for cultivating a nitrile hydratase producing microorganism, compositions for cultivating a nitrile hydratase producing microorganism, and use of compositions comprising a saccharide and an organic acid for cultivating a nitrile hydratase producing microorganism. The composition provided in and to be employed in context with the present invention is particularly suitable for inducing both, growth and nitrile hydratase production of corresponding microorganisms.
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公开(公告)号:US20180148753A1
公开(公告)日:2018-05-31
申请号:US15509993
申请日:2015-06-30
Applicant: BASF SE
Inventor: Vaidotas NAVICKAS , Kai-Uwe BALDENIUS , Michael BREUER , Patricia WILDBERGER , Martin PFEIFFER , Bernd NIDETZKY
IPC: C12P19/02
CPC classification number: C12P19/02 , C07H1/02 , C07H11/04 , C12N9/1051 , C12N9/16 , C12P19/26 , C12Y204/01 , C12Y204/01007 , C12Y301/0301
Abstract: The present invention relates to methods of producing sugar phosphates by enzymatic transphosphorylation, wherein the phosphoryl transfer from phosphate donor substrates to sugar substrates is catalyzed by an enzyme having alpha-glucose-1-phosphatase activity. Furthermore, the present invention relates to a biocatalyst having sucrose phosphorylase as well as alpha-glucose-1-phosphatase activity and its use. The production of an enzyme having alpha-glucose-1-phosphatase activity and its use in the transphosphorylation of sugar substrates is also presented herein.
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公开(公告)号:US20170283840A1
公开(公告)日:2017-10-05
申请号:US15514321
申请日:2015-09-30
Applicant: BASF SE
Inventor: Michael BRAUN , Juergen DAEUWEL , Hans-Juergen LANG , Peter OEDMAN , Kai-Uwe BALDENIUS , Matthias KLEINER , Michael KIEFER , Stephan FREYER , Michael BUDDE
CPC classification number: C12P13/02 , C07C233/09 , C08F20/56 , C08L33/26 , C08L2201/54 , C09K8/588 , C12Y402/01084 , D21H17/375
Abstract: The present invention relates to methods for preparing an aqueous acrylamide solution having a low acrylic acid concentration. In addition, the present invention relates to methods for reducing the acrylic acid concentration of an aqueous acrylamide solution. The methods involve a bioconversion of acrylonitrile to acrylamide in the presence of a biocatalyst, wherein during the bioconversion the content of acrylonitrile is maintained at 0.3 w/w % or more referred to the total weight of the composition in the reactor. Also provided is an aqueous acrylamide solution which is obtained by the methods of the present invention. Furthermore, the present invention is related to an acrylamide homopolymer or copolymer obtained by polymerizing the acrylamide of the aqueous solution.
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公开(公告)号:US20170247726A1
公开(公告)日:2017-08-31
申请号:US15513670
申请日:2015-09-30
Applicant: BASF SE
Inventor: Michael BUDDE , Michael BRAUN , Juergen DAEUWEL , Peter OEDMAN , Kai-Uwe BALDENIUS , Matthias KLEINER , Stephan FREYER
CPC classification number: C12P13/02 , C12N9/78 , C12N9/80 , C12Y305/01004 , C12Y305/05001
Abstract: The present invention relates to means and methods for producing an amide compound from a nitrile compound with less acrylic acid as by-product using a Nitrile hydratase (NHase) and Amidase producing microorganism as biocatalyst. Also provided is an aqueous amide compound obtained by the methods of the invention as well as a composition comprising acrylamide or polyacrylamide as well as a dried microorganism exhibiting a NHase/Amidase activity ratio of at least 400 when being brought into contact with a nitrile compound to convert said nitrile compound into an amide compound.
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