System and method for image acquisition using supervised high quality imaging

    公开(公告)号:US10200625B2

    公开(公告)日:2019-02-05

    申请号:US15115715

    申请日:2015-01-30

    Abstract: An image capture system and method for imaging biological samples disposed in culture media supported by a plate. The system has a calibration module, an image acquisition module and an image presentation module. When the system receives a culture plate for imaging, default values for the culture plate and media are used to begin image acquisition at a given time. The captured image is then used to create a pixel by pixel map of the image. The system inspects the pixel-by-pixel map for saturated pixels and for signal to noise ratio and acquires a new image if the number of saturated pixels is at or above a predetermined threshold or the signal to noise ratio for the pixel is below a predetermined threshold. From this inspection a new value of photon flux and/or exposure time is determined and a new image is captured using the new value and the steps are repeated. Upon a determination that a predetermined threshold signal to noise ratio has been obtained for the non-saturated pixels, or when the predetermined upper threshold for the time interval for image acquisition is elapsed the system provides a final image for the given time.

    A SYSTEM AND METHOD FOR IMAGE ACQUISITION USING SUPERVISED HIGH QUALITY IMAGING
    6.
    发明申请
    A SYSTEM AND METHOD FOR IMAGE ACQUISITION USING SUPERVISED HIGH QUALITY IMAGING 审中-公开
    一种使用高质量图像拍摄的图像采集系统和方法

    公开(公告)号:US20170013181A1

    公开(公告)日:2017-01-12

    申请号:US15115715

    申请日:2015-01-30

    Abstract: An image capture system and method for imaging biological samples disposed in culture media supported by a plate. The system has a calibration module, an image acquisition module and an image presentation module. When the system receives a culture plate for imaging, default values for the culture plate and media are used to begin image acquisition at a given time. The captured image is then used to create a pixel by pixel map of the image. The system inspects the pixel-by-pixel map for saturated pixels and for signal to noise ratio and acquires a new image if the number of saturated pixels is at or above a predetermined threshold or the signal to noise ratio for the pixel is below a predetermined threshold. From this inspection a new value of photon flux and/or exposure time is determined and a new image is captured using the new value and the steps are repeated. Upon a determination that a predetermined threshold signal to noise ratio has been obtained for the non-saturated pixels, or when the predetermined upper threshold for the time interval for image acquisition is elapsed the system provides a final image for the given time.

    Abstract translation: 用于成像放置在由板支撑的培养基中的生物样品的成像捕获系统和方法。 该系统具有校准模块,图像采集模块和图像呈现模块。 当系统接收到用于成像的培养板时,使用培养板和培养基的默认值在给定时间开始图像采集。 然后,捕获的图像用于创建图像的逐像素图。 系统检查饱和像素的像素像素映射和信噪比,并且如果饱和像素的数量等于或高于预定阈值或者像素的信噪比低于预定值,则获取新图像 阈。 从该检查中,确定光子通量和/或曝光时间的新值,并使用新值捕获新图像,并重复该步骤。 在已经获得了对于非饱和像素的预定阈值信噪比的确定之后,或者当经过用于图像获取的时间间隔的预定上限阈值时,系统在给定时间内提供最终图像。

    SYSTEM AND METHOD FOR IMAGE ACQUISITION USING SUPERVISED HIGH QUALITY IMAGING

    公开(公告)号:US20190116303A1

    公开(公告)日:2019-04-18

    申请号:US16218582

    申请日:2018-12-13

    Abstract: An image capture system and method for imaging biological samples disposed in culture media supported by a plate. The system has a calibration module, an image acquisition module and an image presentation module. When the system receives a culture plate for imaging, default values for the culture plate and media are used to begin image acquisition at a given time. The captured image is then used to create a pixel by pixel map of the image. The system inspects the pixel-by-pixel map for saturated pixels and for signal to noise ratio and acquires a new image if the number of saturated pixels is at or above a predetermined threshold or the signal to noise ratio for the pixel is below a predetermined threshold. From this inspection a new value of photon flux and/or exposure time is determined and a new image is captured using the new value and the steps are repeated. Upon a determination that a predetermined threshold signal to noise ratio has been obtained for the non-saturated pixels, or when the predetermined upper threshold for the time interval for image acquisition is elapsed the system provides a final image for the given time.

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