公开(公告)号：US20240020949A1

公开(公告)日：2024-01-18

申请号：US18031928

申请日：2021-10-19

Applicant: BIOMERIEUX ,  BIOASTER

Inventor： Pierre MAHÉ ,  Meriem EL AZAMI ,  Elodie DEGOUT-CHARMETTE ,  Zohreh SEDAGHAT ,  Quentin JOSSO ,  Fabian ROL

IPC: G06V10/764 ,  G06T7/10 ,  G06V10/24 ,  G06V10/44 ,  G06V10/774

CPC classification number: G06V10/764 ,  G06T7/10 ,  G06V10/24 ,  G06V10/44 ,  G06V10/774 ,  G06T2207/20084 ,  G06T2207/20132

Abstract: A method for classifying at least one input image representing a target particle in a sample, involves implementing, by data processing of a client, steps of: (b) extracting the characteristic map of the target particle by a convolutional neural network pre-trained on a base of public images; (c) classifying the input image according to the extracted characteristic map.

公开(公告)号：US20230386233A1

公开(公告)日：2023-11-30

申请号：US18032399

申请日：2021-10-19

Applicant: BIOMERIEUX ,  BIOASTER

Inventor： Pierre MAHÉ ,  Meriem EL AZAMI ,  Elodie DEGOUT-CHARMETTE ,  Zohreh SEDAGHAT ,  Quentin JOSSO ,  Fabian ROL

IPC: G06V20/69 ,  G06V10/82 ,  G06V10/77

CPC classification number: G06V20/698 ,  G06V10/82 ,  G06V10/7715

Abstract: A method for classifying a sequence of input images representing a target particle in a sample over time, includes the following steps performed by the data processing of a client, namely: (b) concatenation of the input images in the sequence as a three-dimensional stack; (c) direct classification of the three-dimensional stack using a convolutional neural network, CNN

公开(公告)号：US20230408977A1

公开(公告)日：2023-12-21

申请号：US18031663

申请日：2021-10-19

Applicant: BIOMERIEUX ,  BIOASTER

Inventor： Zohreh SEDAGHAT ,  Quentin JOSSO ,  Fabian ROL

IPC: G03H1/00

CPC classification number: G03H1/0005 ,  G03H2001/0816

Abstract: A digital holographic imaging technique, includes iterative steps of: a) through back-propagation to the object coordinate of a hologram field comprising a spatial distribution of amplitude corresponding to the spatial distribution of intensity of the hologram and a spatial distribution of phase, determining an object field involving a spatial distribution of absorption and of phase shift of the imaged object, b) thresholding the values of the spatial distribution of absorption and of phase shift by decreasing the values to below a respective threshold, the thresholds decreasing in each iteration, c) through repropagation of the object field to the hologram coordinate, determining a modified hologram field comprising a modified spatial distribution of amplitude and a modified spatial distribution of phase, d) replacing the spatial distribution of phase of the hologram field with the modified spatial distribution of phase, the spatial distribution of phase shift and of absorption of the imaged object being those of the object field of the last iteration.

公开(公告)号：US20230386176A1

公开(公告)日：2023-11-30

申请号：US18031972

申请日：2021-10-19

Applicant: BIOMERIEUX ,  BIOASTER

Inventor： Pierre MAHÉ ,  Meriem EL AZAMI ,  Elodie DEGOUT-CHARMETTE ,  Zohreh SEDAGHAT ,  Quentin JOSSO ,  Fabian ROL

IPC: G06V10/764 ,  G06T7/11 ,  G06V10/24 ,  G06V10/771 ,  G06V20/69

CPC classification number: G06V10/764 ,  G06T7/11 ,  G06V10/24 ,  G06V10/771 ,  G06V20/69 ,  G06V2201/07 ,  G06T2207/20084 ,  G06T2207/20132

Abstract: A method for classifying at least one input image representing a target particle in a sample involves implementing, by data processing a client, steps of: (B) extracting a characteristic map of the target particle from the input image; (c) reducing the number of variables in the extracted characteristic map, using the t-SNE algorithm; (d) classifying, unsupervised, the input image based on the characteristic map having a reduced number of variables.

公开(公告)号：US20230386232A1

公开(公告)日：2023-11-30

申请号：US18031729

申请日：2021-10-19

Applicant: BIOMERIEUX ,  BIOASTER

Inventor： Pierre MAHÉ ,  Meriem EL AZAMI ,  Elodie DEGOUT-CHARMETTE ,  Zohreh SEDAGHAT ,  Quentin JOSSO ,  Fabian ROL

IPC: G06V20/69 ,  G06V10/774

CPC classification number: G06V20/698 ,  G06V20/695 ,  G06V10/774

Abstract: A method for classifying at least one input image containing a target particle in a sample, involves implementing, via data-processing of a client, steps of: (b) extracting a vector of characteristics of the target particle, the characteristics being numerical coefficients each associated with one elementary image of a set of elementary images each representing a reference particle, such that a linear combination of the elementary images weighted by the coefficients approximates the representation of the target particle in the input image; (c) classifying the input image depending on the extracted vector of characteristics.

公开(公告)号：US20200072728A1

公开(公告)日：2020-03-05

申请号：US16066127

申请日：2016-12-27

Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES ,  BIOMERIEUX

Inventor： Francois PERRAUT ,  Quentin JOSSO

IPC: G01N15/14 ,  G03H1/04 ,  G01N21/47

Abstract: A device including a light source, an image sensor, and a holder defining two positions between the light source and the image sensor. Each position is able to receive an object with a view to its observation. An optical system is placed between the two positions. Thus, when an object is placed in a first position, it may be observed, through the optical system, via a conventional microscopy modality. When an object is placed in the second position, it may be observed via a second lensless imagery modality.

公开(公告)号：US20170284926A1

公开(公告)日：2017-10-05

申请号：US15536507

申请日：2015-12-17

Applicant: Commissariat A L'Energie Atomique Et Aux Energies Alternatives ,  Biomerieux

Inventor： Francois PERRAUT ,  Pierre JOLY ,  Quentin JOSSO ,  Meike KLOSTER-LANDSBERG ,  Alice DOUET

IPC: G01N15/14 ,  G03H1/08 ,  G06K9/00 ,  G03H1/00

CPC classification number: G01N15/1475 ,  G01N2015/1445 ,  G03H1/0005 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/005 ,  G03H2001/0883 ,  G06K9/00147

Abstract: The invention concerns a method for identifying biological particles from a stack of holographic images obtained by means of an optical system. A stack of image blocks centred on the biological particle to be analysed is extracted from the stack of images and a reference block corresponding to the focus plan is determined. A characteristic magnitude is calculated for each block of the stack and the profile of this characteristic magnitude along the optical axis of the system is compared with a plurality of standard profiles relative to known types of particle. Alternatively, blocks of the stack are extracted from the stack of blocks for predetermined defocusing deviations and the extracted blocks are compared with standard blocks relative to known types of particle.

公开(公告)号：US20170309036A1

公开(公告)日：2017-10-26

申请号：US15526092

申请日：2015-11-13

Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES ,  BIOMERIEUX

Inventor： Francois PERRAUT ,  Pierre JOLY ,  Quentin JOSSO ,  Meike KLOSTER-LANDSBERG

IPC: G06T7/55 ,  G06T15/10 ,  G06T7/00

CPC classification number: G06T7/55 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N21/453 ,  G01N21/4788 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1445 ,  G01N2015/1452 ,  G01N2015/1454 ,  G03H1/0005 ,  G03H1/041 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/0033 ,  G03H2001/005 ,  G03H2001/0428 ,  G03H2001/0447 ,  G03H2001/0452 ,  G03H2001/0467 ,  G03H2001/0883 ,  G06T7/97 ,  G06T15/10

Abstract: A method of analyzing a sample receiving a particle of interest, including: defining a reference point located on a first interface of the sample, or at a known distance from the sample, along the optical axis of the optical system; acquiring a reference image transmission of the sample, the object plane of the optical system being located at a known distance from the reference point along an axis parallel to the optical axis of the optical system, and the particle of interest being located outside of the object plane; using the reference image, digitally constructing a series of reconstructed images, each associated with a predetermined offset of the object plane along the optical axis of the optical system; and using the series of reconstructed images, determining the distance along an axis parallel to the optical axis of the optical system, between the particle of interest and the reference point.

公开(公告)号：US20190120747A1

公开(公告)日：2019-04-25

申请号：US15772563

申请日：2016-11-03

Applicant: Commissariat A L'Energie Atomique Et Aux Energies Alternatives ,  BIOMERIEUX

Inventor： Mickael DOMENES ,  Quentin JOSSO ,  Francois PERRAUT

IPC: G01N15/14 ,  G03H1/04

Abstract: A device and a method for observing an object by imaging, or by lensless imaging. The object is retained by a holder defining an object plane inserted between a light source and an image sensor, with no enlargement optics being placed between the object and the image sensor. An optical system is arranged between the light source and the holder and is configured to form a convergent incident wave from a light wave emitted by the light source, and for forming a secondary light source, conjugated with the light source, positioned in a half-space defined by the object plane and including the image sensor, such that the secondary source is closer to the image sensor than to the holder. This results in an image with a transversal enlargement factor having an absolute value of less than 1.

公开(公告)号：US20170363533A1

公开(公告)日：2017-12-21

申请号：US15540068

申请日：2015-10-23

Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES ,  BIOMERIEUX

Inventor： Francois PERRAUT ,  Pierre JOLY ,  Quentin JOSSO ,  Meike KLOSTER-LANDSBERG

IPC: G01N15/14 ,  G06T7/00 ,  G01N15/10 ,  G01N15/00

CPC classification number: G01N15/1434 ,  G01N15/1463 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1452 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/005 ,  G03H2001/0428 ,  G03H2001/0447 ,  G03H2001/0469 ,  G03H2001/0883 ,  G03H2227/03 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: A system for analyzing a transparent particle including: an analysis pathway, including a first light source emitting an analysis light beam, and a first optical system focusing the analysis light beam in a focusing plane; and a position control pathway including a second light source, an image sensor, and a second optical system at least partially merged with the first optical system. The image sensor is offset relative to the image of the focusing plane by the second optical system. The system makes it possible to control correct positioning of the particle, even though it is transparent, and without disturbing the analysis pathway.