公开(公告)号：US11965208B2

公开(公告)日：2024-04-23

申请号：US16849191

申请日：2020-04-15

Applicant: Becton, Dickinson and Company

Inventor： Christina Chang ,  Sixing Li

IPC: C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6858

CPC classification number: C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Disclosed herein include systems, methods, compositions, and kits for associating single cell sequencing data with phenotypic data and/or agent exposure. In some embodiments, cell indexing oligonucleotides comprising cell indexing sequences are associated with cells. In some embodiments, synthetic particles are associated with stochastic labels comprising cell indexing sequences. Sequential hybridizing and imaging of probes annealing to a cell indexing sequence can generate a spatial index. The spatial index can be employed to identify of the partition from which a sequenced nucleic acid target molecule originated.

公开(公告)号：US20160289669A1

公开(公告)日：2016-10-06

申请号：US15004618

申请日：2016-01-22

Applicant: Becton, Dickinson and Company

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoff Facer ,  David Stern ,  Janice Lai ,  Ari Chaney ,  Phillip Spuhler ,  Sixing Li ,  Xiaohua Chen Huang ,  Kimberly R. Metzler ,  Elisabeth Marie Walczak

IPC: C12N15/10 ,  C12Q1/68 ,  B01L3/00

CPC classification number: C12N15/1065 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/026 ,  B01L2200/0647 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/12 ,  B01L2300/18 ,  B01L2400/082 ,  C12Q1/6846 ,  C12Q1/6874 ,  G01N35/0098 ,  C12Q2521/107 ,  C12Q2525/173 ,  C12Q2525/185 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2563/143 ,  C12Q2563/149

Abstract: Disclosed herein are devices and systems comprising a) a substrate comprising at least 100 microwells and a plurality of beads, wherein a plurality of the at least 100 microwells each contain a single bead, and wherein the ratio of the average diameter of the microwells to the diameter of the beads ranges from about 1.2 to about 1.8; b) a flow cell in fluid communication with the substrate; and c) at least one inlet port and at least one outlet port, wherein the at least one inlet port and at least one outlet port are capable of directing a flow of a fluid through the flow cell, thereby contacting the microwells with the fluid.

Abstract translation: 本文公开的装置和系统包括a）包含至少100个微孔和多个珠的基底，其中多个至少100个微孔各自包含单个珠，并且其中微孔的平均直径与 珠的直径范围为约1.2至约1.8; b）与衬底流体连通的流动池; 以及c）至少一个入口端口和至少一个出口端口，其中所述至少一个入口端口和至少一个出口端口能够引导流体流过所述流动池，从而使所述微孔与所述流体接触。

公开(公告)号：US20200332351A1

公开(公告)日：2020-10-22

申请号：US16849191

申请日：2020-04-15

Applicant: Becton, Dickinson and Company

Inventor： Christina Chang ,  Sixing Li

IPC: C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6858

Abstract: Disclosed herein include systems, methods, compositions, and kits for associating single cell sequencing data with phenotypic data and/or agent exposure. In some embodiments, cell indexing oligonucleotides comprising cell indexing sequences are associated with cells. In some embodiments, synthetic particles are associated with stochastic labels comprising cell indexing sequences. Sequential hybridizing and imaging of probes annealing to a cell indexing sequence can generate a spatial index. The spatial index can be employed to identify of the partition from which a sequenced nucleic acid target molecule originated.