公开(公告)号：US20200171501A1

公开(公告)日：2020-06-04

申请号：US16661310

申请日：2019-10-23

Applicant: Berkeley Lights, Inc.

Inventor： Jason M. McEwen ,  Magali Soumillon ,  Shao Ning Pei ,  Randall D. Lowe, Jr. ,  Samira A. Nedungadi ,  Volker L.S. Kurz ,  Jian Gong ,  Yara X. Mejia Gonzalez ,  Mckenzi S. Toh ,  Brian A. Rabkin ,  Jason C. Briggs ,  Darcy K. Kelly-Greene ,  James M. Porter, Jr.

IPC: B01L3/00 ,  B01L7/00 ,  B03C5/00 ,  B03C5/02

Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided for processing biological cells, e.g., for use in nucleic acid library preparation and/or synthesis (including amplification). The devices include a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode. Methods of nucleic acid library preparation and/or synthesis can involve providing reagents to cells or nucleic acids by merging appropriate droplets on a droplet actuation surface within a water-immiscible organic liquid and can be performed in the presence of appropriate surfactants. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are systems for temperature control of the microfluidic device during nucleic acid library preparation and/or synthesis which can reduce temperature overshooting during heating and cooling steps.

公开(公告)号：US20180147576A1

公开(公告)日：2018-05-31

申请号：US15802174

申请日：2017-11-02

Applicant: Berkeley Lights, Inc.

Inventor： Gregory G. Lavieu ,  Annamaria Mocciaro ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Magali Soumillon ,  J. Tanner Nevill ,  Volker L.S. Kurz ,  Patricia A. Dyck ,  Ravi K. Ramenani

IPC: B01L3/00 ,  C12M3/06 ,  C12N15/10 ,  G01N15/10

CPC classification number: B01L3/502761 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12M23/16 ,  C12N15/1003 ,  C12N15/1024 ,  C12N15/1086 ,  C12N2310/00 ,  C12N2510/00 ,  G01N15/10 ,  G01N2015/0288 ,  G01N2015/1081

Abstract: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

公开(公告)号：US20230092258A1

公开(公告)日：2023-03-23

申请号：US17807597

申请日：2022-06-17

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. Kurz ,  John A. Tenney ,  Long Van Le

IPC: B03C5/00 ,  B01L3/00 ,  G01N15/14 ,  B03C5/02

Abstract: Methods of selectively positioning a micro-object in a microfluidic device are described in this application. The microfluidic device can comprise an enclosure having an inlet, an outlet, and a flow region connecting the inlet and outlet, and an electrode activation substrate having a photoconductive layer. The methods of selectively positioning can comprising: projecting a first light beam on an electrode activation substrate of the microfluidic device, wherein the first position is proximal to the first micro-object, and wherein the first light beam activates a positive dielectrophoresis (DEP) force within the enclosure sufficient to capture the first micro-object; and projecting a second light beam upon a second position on the electrode activation substrate, wherein the second position is adjacent to or at least partially surrounding the first position, without overlapping the first position, the second light beam activating a positive DEP force within the enclosure sufficient to capture second micro-objects other than the first micro-object. The methods of selectively positioning can further comprise moving the first light beam towards a third position on the electrode activation substrate, wherein the DEP force activated by the first light beam is sufficient to move the first micro-object to the third position. Optionally, the methods can include moving the second light beam in relation to the first light beam to prevent micro-objects other than the first micro-object from being captured by the first light beam. Other embodiments are described.

公开(公告)号：US20210011015A1

公开(公告)日：2021-01-14

申请号：US16908265

申请日：2020-06-22

Applicant: Berkeley Lights, Inc.

Inventor： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L.S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/545 ,  G01N33/58

Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US20230182136A1

公开(公告)日：2023-06-15

申请号：US18164124

申请日：2023-02-03

Applicant: BERKELEY LIGHTS, INC.

Inventor： Kristin G. Beaumont ,  Non-Linda Ding ,  Volker L.S. Kurz ,  Troy A. Lionberger ,  Randall D. Lowe, JR. ,  Daniele Malleo ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: B01L3/00 ,  B01J19/00 ,  G01N27/447

CPC classification number: B01L3/502753 ,  B01L3/502761 ,  B01L3/502738 ,  B01J19/0093 ,  B01L3/5023 ,  B01L3/502707 ,  G01N27/44791 ,  B01L2400/0424 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0677 ,  B01L2200/0668 ,  B01L2300/16 ,  B01L2400/08

Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.

公开(公告)号：US20190217297A1

公开(公告)日：2019-07-18

申请号：US16259538

申请日：2019-01-28

Applicant: Berkeley Lights, Inc.

Inventor： Gregory G. Lavieu ,  Annamaria Mocciaro ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Magali Soumillon ,  J. Tanner Nevill ,  Volker L.S. Kurz ,  Patricia A. Dyck ,  Ravi K. Ramenani

IPC: B01L3/00 ,  G01N15/10 ,  C12N15/10 ,  C12M3/06

CPC classification number: B01L3/502761 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12M23/16 ,  C12N15/1003 ,  C12N15/1024 ,  C12N15/1086 ,  C12N2310/00 ,  C12N2510/00 ,  G01N15/10 ,  G01N2015/0288 ,  G01N2015/1081

Abstract: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.

公开(公告)号：US20170184583A1

公开(公告)日：2017-06-29

申请号：US15372094

申请日：2016-12-07

Applicant: Berkeley Lights, Inc.

Inventor： Kristin G. Beaumont ,  Peter J. Beemiller ,  Volker L.S. Kurz ,  Gregory G. Lavieu ,  Xiaohua Wang ,  Aathavan Karunakaran

IPC: G01N33/543 ,  G01N33/58 ,  G01N33/545

Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US20210102150A1

公开(公告)日：2021-04-08

申请号：US17067971

申请日：2020-10-12

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. Kurz ,  Troy A. Lionberger ,  Eric K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/26

Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

公开(公告)号：US20180298318A1

公开(公告)日：2018-10-18

申请号：US16010176

申请日：2018-06-15

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. Kurz ,  Troy A. Lionberger ,  Erik K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/26

CPC classification number: C12M23/16 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0442 ,  B01L2400/0454 ,  B01L2400/086 ,  C12M23/20 ,  C12M33/12

Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

公开(公告)号：US20170165667A1

公开(公告)日：2017-06-15

申请号：US15359115

申请日：2016-11-22

Applicant: Berkeley Lights, Inc.

Inventor： Kristin G. Beaumont ,  Nan-Linda Ding ,  Volker L.S. Kurz ,  Troy A. Lionberger ,  Randall D. Lowe ,  Daniele Malleo ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: B01L3/00 ,  B01J19/00 ,  G01N27/447

CPC classification number: B01L3/502753 ,  B01J19/0093 ,  B01L3/5023 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/16 ,  B01L2400/0424 ,  B01L2400/0677 ,  B01L2400/08 ,  G01N27/44791

Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.