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公开(公告)号:US20180135011A1
公开(公告)日:2018-05-17
申请号:US15802100
申请日:2017-11-02
发明人: Yelena Bronevetsky , Xiaohua Wang , Peter J. Beemiller , Kristin G. Beaumont , Randall D. Lowe, JR. , Alexander J. Mastroianni , Kevin T. Chapman
IPC分类号: C12N5/0783 , A61P35/00 , C12M3/06 , A61K39/00 , C12M1/00
CPC分类号: C12N5/0636 , A61K35/17 , A61K39/0011 , A61K2035/124 , A61K2039/5158 , A61P35/00 , C12M23/16 , C12M29/10 , C12N2501/2302 , C12N2501/51 , C12N2501/515 , C12N2502/1121 , C12N2533/50
摘要: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.
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2.
公开(公告)号:US20210102150A1
公开(公告)日:2021-04-08
申请号:US17067971
申请日:2020-10-12
发明人: Volker L.S. Kurz , Troy A. Lionberger , Eric K. Sackmann , Kai W. Szeto , Paul M. Lebel , Brandon R. Bruhn , Keith J. Breinlinger , Eric D. Hobbs , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
摘要: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.
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公开(公告)号:US20210069698A1
公开(公告)日:2021-03-11
申请号:US16928225
申请日:2020-07-14
发明人: Xiao Guan , Mark P. White , Jason M. McEwen , Gang F. Wang , Kevin T. Chapman , Xiaohua Wang , Christine E. Sun
摘要: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.
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4.
公开(公告)号:US10705082B2
公开(公告)日:2020-07-07
申请号:US15372094
申请日:2016-12-07
发明人: Kristin G. Beaumont , Peter J. Beemiller , Volker L. S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号: B01L3/00 , G01N33/543 , G01N33/545 , G01N33/58
摘要: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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5.
公开(公告)号:US20180298318A1
公开(公告)日:2018-10-18
申请号:US16010176
申请日:2018-06-15
发明人: Volker L.S. Kurz , Troy A. Lionberger , Erik K. Sackmann , Kai W. Szeto , Paul M. Lebel , Brandon R. Bruhn , Keith J. Breinlinger , Eric D. Hobbs , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
CPC分类号: C12M23/16 , B01L3/502761 , B01L2200/0668 , B01L2300/1822 , B01L2400/0415 , B01L2400/0427 , B01L2400/0442 , B01L2400/0454 , B01L2400/086 , C12M23/20 , C12M33/12
摘要: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.
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6.
公开(公告)号:US20170276679A1
公开(公告)日:2017-09-28
申请号:US15406289
申请日:2017-01-13
发明人: Kevin T. Chapman , Mark P. White , Xiaohua Wang , Minha Park , Guido K. Stadler , Randall D. Lowe, Jr. , Xiao Guan , Jason M. McEwen , Gang Wang , George L. Fox , Peggy A. Radel
IPC分类号: G01N33/574 , C12Q1/68 , G01N33/543 , B01L3/00
摘要: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.
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公开(公告)号:US20170165667A1
公开(公告)日:2017-06-15
申请号:US15359115
申请日:2016-11-22
发明人: Kristin G. Beaumont , Nan-Linda Ding , Volker L.S. Kurz , Troy A. Lionberger , Randall D. Lowe , Daniele Malleo , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
IPC分类号: B01L3/00 , B01J19/00 , G01N27/447
CPC分类号: B01L3/502753 , B01J19/0093 , B01L3/5023 , B01L3/502707 , B01L3/502738 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/16 , B01L2400/0424 , B01L2400/0677 , B01L2400/08 , G01N27/44791
摘要: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.
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8.
公开(公告)号:US11454629B2
公开(公告)日:2022-09-27
申请号:US16908265
申请日:2020-06-22
发明人: Kristin G. Beaumont , Peter J. Beemiller , Volker L. S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号: B01L3/00 , G01N33/543 , G01N33/545 , G01N33/58 , G01N33/50
摘要: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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9.
公开(公告)号:US20170184583A1
公开(公告)日:2017-06-29
申请号:US15372094
申请日:2016-12-07
发明人: Kristin G. Beaumont , Peter J. Beemiller , Volker L.S. Kurz , Gregory G. Lavieu , Xiaohua Wang , Aathavan Karunakaran
IPC分类号: G01N33/543 , G01N33/58 , G01N33/545
摘要: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.
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公开(公告)号:US10751715B1
公开(公告)日:2020-08-25
申请号:US15136481
申请日:2016-04-22
发明人: Xiao Guan , Mark P. White , Jason M. McEwen , Gang F. Wang , Kevin T. Chapman , Xiaohua Wang , Christine E. Sun
摘要: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.
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