公开(公告)号：US10167509B2

公开(公告)日：2019-01-01

申请号：US14848311

申请日：2015-09-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Serge Saxonov ,  Michael Y. Lucero ,  Benjamin J. Hindson ,  Phillip Belgrader ,  Simant Dube ,  Austin P. So ,  Jeffrey C. Mellen ,  Nicholas J. Heredia ,  Kevin D. Ness ,  Billy W. Colston, Jr.

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6883 ,  C12Q1/683 ,  C12Q1/6858

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

公开(公告)号：US09702822B2

公开(公告)日：2017-07-11

申请号：US13973940

申请日：2013-08-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Claudia Litterst ,  Austin P. So ,  Duc Do

IPC: C12Q1/68 ,  C12P19/34 ,  G01N21/64

CPC classification number: G01N21/6486 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2525/204 ,  C12Q2563/159

Abstract: Digital assay system, includes methods, apparatus, and compositions, for distinguishing and measuring different types of templates according to the different lengths of corresponding amplicons, which may be amplified by the same pair of primers. The system may include a length-sensitive reporter generating luminescence that varies according to amplicon length. The system may, for example, be utilized to identify, distinguish, and/or quantify wild-type and mutant/variant templates, processed and unprocessed template, a target template and a primer dimer, or the like.

公开(公告)号：US20140057273A1

公开(公告)日：2014-02-27

申请号：US13973940

申请日：2013-08-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Claudia Litterst ,  Austin P. So ,  Duc Do

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  C12Q1/6846 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2525/204 ,  C12Q2563/159

Abstract: Digital assay system, includes methods, apparatus, and compositions, for distinguishing and measuring different types of templates according to the different lengths of corresponding amplicons, which may be amplified by the same pair of primers. The system may include a length-sensitive reporter generating luminescence that varies according to amplicon length. The system may, for example, be utilized to identify, distinguish, and/or quantify wild-type and mutant/variant templates, processed and unprocessed template, a target template and a primer dimer, or the like.

Abstract translation: 数字测定系统包括用于根据相应扩增子的不同长度区分和测量不同类型的模板的方法，装置和组合物，其可以通过相同的引物对扩增。 该系统可以包括长度敏感的报告基因产生根据扩增子长度而变化的发光。 该系统可以例如用于鉴定，区分和/或定量野生型和突变体/变体模板，加工和未处理的模板，靶模板和引物二聚体等。

公开(公告)号：US20160076099A1

公开(公告)日：2016-03-17

申请号：US14848311

申请日：2015-09-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Serge Saxonov ,  Michael Y. Lucero ,  Benjamin J. Hindson ,  Phillip Belgrader ,  Simant Dube ,  Austin P. So ,  Jeffrey C. Mellen ,  Nicholas J. Heredia ,  Kevin D. Ness ,  Billy W. Colston, Jr.

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/683 ,  C12Q1/6858 ,  C12Q2600/158 ,  C12Q2600/172 ,  C12Q2537/16 ,  C12Q2563/159 ,  C12Q2537/165

Abstract: Provided herein are improved methods, compositions, and kits for analysis of nucleic acids. The improved methods, compositions, and kits can enable copy number estimation of a nucleic acid in a sample. Also provided herein are methods, compositions, and kits for determining the linkage of two or more copies of a target nucleic acid in a sample (e.g. whether the two or more copies are on the same chromosome or different chromosomes) or for phasing alleles.

Abstract translation: 本文提供了用于分析核酸的改进的方法，组合物和试剂盒。 改进的方法，组合物和试剂盒可以使得样品中核酸的拷贝数估计可以进行。 本文还提供了用于确定样品中靶核酸的两个或多个拷贝（例如两个或多个拷贝是在同一染色体上还是在不同染色体上）或用于定相等位基因的连接的方法，组合物和试剂盒。

公开(公告)号：US11499181B2

公开(公告)日：2022-11-15

申请号：US16235996

申请日：2018-12-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Serge Saxonov ,  Michael Y. Lucero ,  Benjamin J. Hindson ,  Phillip Belgrader ,  Simant Dube ,  Austin P. So ,  Jeffrey C. Mellen ,  Nicholas J. Heredia ,  Kevin D. Ness ,  Billy W. Colston, Jr.

IPC: C12Q1/6858 ,  C12Q1/683 ,  C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/686

Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.

公开(公告)号：US20190241947A1

公开(公告)日：2019-08-08

申请号：US16235996

申请日：2018-12-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. Regan ,  Serge Saxonov ,  Michael Y. Lucero ,  Benjamin J. Hindson ,  Phillip Belgrader ,  Simant Dube ,  Austin P. So ,  Jeffrey C. Mellen ,  Nicholas J. Heredia ,  Kevin D. Ness ,  Billy W. Colston, JR.

IPC: C12Q1/6858 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6827

CPC classification number: C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2535/125 ,  C12Q2600/158 ,  C12Q2600/172 ,  C12Q2537/16 ,  C12Q2563/159 ,  C12Q2537/165

Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.