公开(公告)号：US12239987B2

公开(公告)日：2025-03-04

申请号：US17986873

申请日：2022-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. Stumbo ,  George Carman ,  Steve Hobbs ,  Anthony J. Makarewicz, Jr. ,  Dmitri Simonian ,  David Glade ,  Joshua Oen ,  Denis Pristinski ,  John Dzenitis

IPC: B01L3/00 ,  B01F23/41 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1434

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US11499183B2

公开(公告)日：2022-11-15

申请号：US16022500

申请日：2018-06-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. Stumbo ,  George Carman ,  Steve Hobbs ,  Anthony J. Makarewicz, Jr. ,  Dmitri Simonian ,  David Glade ,  Joshua Oen ,  Denis Pristinski ,  John Dzenitis

IPC: G01N15/14 ,  B01F3/08 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/686 ,  B01L3/00 ,  C12Q1/6851 ,  B01F23/41

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US11779925B2

公开(公告)日：2023-10-10

申请号：US17549163

申请日：2021-12-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas Henry Cauley, III ,  John Dzenitis ,  Steve Hobbs ,  Stefano Schiaffino

IPC: B01L3/00 ,  G01N1/38 ,  B01F23/41 ,  B01F33/85 ,  B01L9/00 ,  B01F101/23

CPC classification number: B01L3/502784 ,  B01F23/4105 ,  B01F33/85 ,  B01L3/502715 ,  G01N1/38 ,  B01F2101/23 ,  B01L9/527 ,  B01L2200/028 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2400/049

Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.

公开(公告)号：US11207685B2

公开(公告)日：2021-12-28

申请号：US15894836

申请日：2018-02-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas Henry Cauley, III ,  John Dzenitis ,  Steve Hobbs ,  Stefano Schiaffino

IPC: B01L3/00 ,  G01N1/38 ,  B01F3/08 ,  B01F13/10 ,  B01L9/00

Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.