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公开(公告)号：US20140221239A1
公开(公告)日：2014-08-07
申请号：US14171766
申请日：2014-02-03
摘要： System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.
摘要翻译： 系统，包括用于将液滴彼此间隔开并用于检测间隔液滴的方法和装置。
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公开(公告)号：US11499183B2
公开(公告)日：2022-11-15
申请号：US16022500
申请日：2018-06-28
发明人： David P. Stumbo , George Carman , Steve Hobbs , Anthony J. Makarewicz, Jr. , Dmitri Simonian , David Glade , Joshua Oen , Denis Pristinski , John Dzenitis
IPC分类号： G01N15/14 , B01F3/08 , C12Q1/6848 , C12Q1/6844 , C12Q1/686 , B01L3/00 , C12Q1/6851 , B01F23/41
摘要： Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.
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公开(公告)号：US20170191923A1
公开(公告)日：2017-07-06
申请号：US15394624
申请日：2016-12-29
发明人： Yunfeng Ling , David P. Stumbo , George Carman , Denis Pristinski
IPC分类号： G01N15/14
CPC分类号： G01N15/1429 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N2015/1488
摘要： Systems and methods for detecting and processing signals from particles. In an exemplary method, particles may be passed through a zone of a channel, while the zone is irradiated with light. Interaction of the light with the particles may deflect light and induce photoluminescence. A deflection signal and a photoluminescence signal may be detected from the zone. Particle waveforms may be identified in the deflection signal. At least a subset of the particle waveforms may be double-peak waveforms including a pair of peaks corresponding to a particle entering and exiting the zone. Amplitudes may be obtained from the photoluminescence signal. The amplitudes may correspond to respective particles and their particle waveforms, and at least a subset of the amplitudes may correspond to the double-peak waveforms. Individual particles may be assigned as positive or as negative for an analyte based on the corresponding amplitudes.
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公开(公告)号：US11878302B2
公开(公告)日：2024-01-23
申请号：US17465763
申请日：2021-09-02
IPC分类号： B01L3/00
CPC分类号： B01L3/502784 , B01L2200/0647 , B01L2200/0673 , B01L2300/0816 , B01L2300/0867 , B01L2400/0487
摘要： System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.
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公开(公告)号：US20210394189A1
公开(公告)日：2021-12-23
申请号：US17465763
申请日：2021-09-02
IPC分类号： B01L3/00
摘要： System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.
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公开(公告)号：US11110458B2
公开(公告)日：2021-09-07
申请号：US14171766
申请日：2014-02-03
摘要： System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.
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公开(公告)号：US10060847B2
公开(公告)日：2018-08-28
申请号：US15394624
申请日：2016-12-29
发明人： Yunfeng Ling , David P. Stumbo , George Carman , Denis Pristinski
IPC分类号： G01N15/14
CPC分类号： G01N15/1429 , G01N15/1404 , G01N15/1434 , G01N15/1459 , G01N2015/1461 , G01N2015/1488 , G01N2021/4707 , G01N2021/4728
摘要： Systems and methods for detecting and processing signals from particles. In an exemplary method, particles may be passed through a zone of a channel, while the zone is irradiated with light. Interaction of the light with the particles may deflect light and induce photoluminescence. A deflection signal and a photoluminescence signal may be detected from the zone. Particle waveforms may be identified in the deflection signal. At least a subset of the particle waveforms may be double-peak waveforms including a pair of peaks corresponding to a particle entering and exiting the zone. Amplitudes may be obtained from the photoluminescence signal. The amplitudes may correspond to respective particles and their particle waveforms, and at least a subset of the amplitudes may correspond to the double-peak waveforms. Individual particles may be assigned as positive or as negative for an analyte based on the corresponding amplitudes.
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