公开(公告)号：US20170189908A1

公开(公告)日：2017-07-06

申请号：US15395199

申请日：2016-12-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Dzenitis ,  Stefano Schiaffino

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L3/0241 ,  B01L3/545 ,  B01L2200/12 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/021 ,  B01L2300/0663 ,  B01L2300/0864 ,  B01L2400/049

Abstract: The present disclosure provides systems and methods for performing droplet assays with automatic calibration. An exemplary assay system may comprise a cartridge including a plurality of droplet generators to form emulsions of droplets having a same nominal volume. A tag may be associated with the cartridge and may encode calibration data or an identifier thereof. The calibration data may include a respective value specific to each droplet generator. The system further may include a detection system to detect a signal representing an analyte from droplets of each emulsion, and a reader to read the calibration data or the identifier from the tag. The system still further may include a processor configured to receive the signal and the calibration data and to calculate, for each emulsion, a concentration of an analyte using at least the signal and the respective value specific to the droplet generator that formed the emulsion.

公开(公告)号：US12239987B2

公开(公告)日：2025-03-04

申请号：US17986873

申请日：2022-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. Stumbo ,  George Carman ,  Steve Hobbs ,  Anthony J. Makarewicz, Jr. ,  Dmitri Simonian ,  David Glade ,  Joshua Oen ,  Denis Pristinski ,  John Dzenitis

IPC: B01L3/00 ,  B01F23/41 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1434

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US11499183B2

公开(公告)日：2022-11-15

申请号：US16022500

申请日：2018-06-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. Stumbo ,  George Carman ,  Steve Hobbs ,  Anthony J. Makarewicz, Jr. ,  Dmitri Simonian ,  David Glade ,  Joshua Oen ,  Denis Pristinski ,  John Dzenitis

IPC: G01N15/14 ,  B01F3/08 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/686 ,  B01L3/00 ,  C12Q1/6851 ,  B01F23/41

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US20180209874A1

公开(公告)日：2018-07-26

申请号：US15881559

申请日：2018-01-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sean Cater ,  John Dzenitis ,  Stefano Schiaffino ,  Pallavi Shah ,  Andy Utada

IPC: G01N1/18 ,  C12Q1/6806 ,  B01L7/00

CPC classification number: G01N1/18 ,  B01L3/0241 ,  B01L7/52 ,  B01L2200/0673 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N35/028 ,  G01N35/1016 ,  G01N2035/0422 ,  G01N2035/1034 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: An assay performance system may include modules configured to store aqueous sample plates, conduct droplet generation or emulsification of aqueous samples, and to perform thermocycling and droplet reading functions. One or more samples may be emulsified and stored in an emulsified state for extended times prior to thermocycling. Accordingly, the assay performance system may include material handling systems and methods to accommodate the storage function.

公开(公告)号：US11779925B2

公开(公告)日：2023-10-10

申请号：US17549163

申请日：2021-12-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas Henry Cauley, III ,  John Dzenitis ,  Steve Hobbs ,  Stefano Schiaffino

IPC: B01L3/00 ,  G01N1/38 ,  B01F23/41 ,  B01F33/85 ,  B01L9/00 ,  B01F101/23

CPC classification number: B01L3/502784 ,  B01F23/4105 ,  B01F33/85 ,  B01L3/502715 ,  G01N1/38 ,  B01F2101/23 ,  B01L9/527 ,  B01L2200/028 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2400/049

Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.

公开(公告)号：US11207685B2

公开(公告)日：2021-12-28

申请号：US15894836

申请日：2018-02-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas Henry Cauley, III ,  John Dzenitis ,  Steve Hobbs ,  Stefano Schiaffino

IPC: B01L3/00 ,  G01N1/38 ,  B01F3/08 ,  B01F13/10 ,  B01L9/00

Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.