公开(公告)号：US20180274029A1

公开(公告)日：2018-09-27

申请号：US15926341

申请日：2018-03-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tal RAZ ,  Pascaline MARY

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6848

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2527/107

Abstract: The present invention relates to methods of target nucleic acid amplification and uses thereof in methods of sequencing, or other down-stream applications such a genotyping or cloning. More specifically, the methods relate to methods of target nucleic acid amplification through the use of a universal hairpin primer.

公开(公告)号：US20170361322A1

公开(公告)日：2017-12-21

申请号：US15691196

申请日：2017-08-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adnan ESMAIL ,  Tal RAZ ,  John HEALY ,  Tony HUNG ,  Sepehr KIANI ,  Pascaline MARY

IPC: B01L3/00 ,  G01N35/08 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2300/1861 ,  B01L2400/0415 ,  B01L2400/0433 ,  B01L2400/0448 ,  B01L2400/088 ,  C12Q1/6869 ,  G01N35/08 ,  Y10T436/143333 ,  Y10T436/2575

Abstract: The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.