公开(公告)号：US20180369818A1

公开(公告)日：2018-12-27

申请号：US15986718

申请日：2018-05-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sepehr KIANI ,  Joshua BLOUWOLFF ,  Adnan ESMAIL ,  Jason HUNG ,  Tony HUNG ,  Adam R. ABATE ,  Scott POWERS ,  Pascaline MARY

IPC: B01L3/00 ,  C12N15/10 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0672 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  B01L2400/0688 ,  B01L2400/084 ,  C12N15/1075

Abstract: Methods and systems for manipulating drops in microfluidic channels are provided.

公开(公告)号：US20180274029A1

公开(公告)日：2018-09-27

申请号：US15926341

申请日：2018-03-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tal RAZ ,  Pascaline MARY

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6848

CPC classification number: C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2527/107

Abstract: The present invention relates to methods of target nucleic acid amplification and uses thereof in methods of sequencing, or other down-stream applications such a genotyping or cloning. More specifically, the methods relate to methods of target nucleic acid amplification through the use of a universal hairpin primer.

公开(公告)号：US20170361322A1

公开(公告)日：2017-12-21

申请号：US15691196

申请日：2017-08-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adnan ESMAIL ,  Tal RAZ ,  John HEALY ,  Tony HUNG ,  Sepehr KIANI ,  Pascaline MARY

IPC: B01L3/00 ,  G01N35/08 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2300/18 ,  B01L2300/1861 ,  B01L2400/0415 ,  B01L2400/0433 ,  B01L2400/0448 ,  B01L2400/088 ,  C12Q1/6869 ,  G01N35/08 ,  Y10T436/143333 ,  Y10T436/2575

Abstract: The present invention provides a microfluidic system, method and kit for performing assays. The system may comprise a microfluidic device and a detector, wherein the assay yields results that may be read by a detector and analyzed by the system. The assay may comprise one or more chemical or biological reaction against, or performed on, a sample or multiple samples. The sample(s) may become larger and/or smaller during the performance of the assay. The sample(s) may be present within a vehicle, or on a carrier within a vehicle, in the microfluidic device, and wherein the vehicle may become larger and/or smaller during the performance of the assay. The assay may be a cascading assay comprising a series of multiple assays, wherein each assay may be the same or different, and wherein each assay in the series of multiple assays may further comprise one or more process or step.

公开(公告)号：US20180078933A1

公开(公告)日：2018-03-22

申请号：US15822742

申请日：2017-11-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam ABATE ,  Sepehr KIANI ,  Tony HUNG ,  Pascaline MARY ,  Adnan Moez ESMAIL

IPC: B01L3/00 ,  F16K99/00 ,  B01F13/00 ,  F04B19/00

CPC classification number: B01L3/5027 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502784 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1861 ,  B01L2400/0424 ,  B01L2400/0436 ,  B01L2400/0442 ,  F04B19/006 ,  F16K99/0019

Abstract: The present invention generally pertains to a system for performing injection of multiple substantially controlled volumes into or out of a droplet, and methods and kits comprising the same. The system of the present invention comprises at least one microfluidic channel, one or more injection channels, an injection inlet associated with each of the one or more injection channels, and a mechanism for disrupting an interface between a droplet and a fluid and/or emulsion, wherein the at least one microfluidic channel comprises one or more droplets are flowing therein, and wherein each of the one or more injection channels comprises at least one fluid and/or emulsion therein.

公开(公告)号：US20180045648A1

公开(公告)日：2018-02-15

申请号：US15722486

申请日：2017-10-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam ABATE ,  John Lawrence EMHOFF ,  Tony HUNG ,  Adnan Moez ESMAIL ,  Sepehr KIANI ,  Pascaline MARY

IPC: G01N21/64 ,  C12Q1/68 ,  G01N21/25 ,  G01N21/76

CPC classification number: G01N21/6486 ,  C12Q1/6806 ,  G01N21/255 ,  G01N21/76 ,  G01N2201/06113 ,  G01N2201/062 ,  C12Q2545/101 ,  C12Q2563/107 ,  C12Q2563/159 ,  C12Q2563/161 ,  C12Q2565/629

Abstract: The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein.