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    Recombinant expression vectors for expression of heterologous proteins
    4.
    发明授权
    Recombinant expression vectors for expression of heterologous proteins 失效
    用于表达异源蛋白质的重组表达载体

    公开(公告)号：US5846819A

    公开(公告)日：1998-12-08

    申请号：US472045

    申请日：1995-06-06

    申请人： Mark H. Pausch ,  Bradley A. Ozenberger ,  John R. Hadcock ,  Laura A. Price ,  Eileen M. Kajkowski ,  Donald R. Kirsch ,  Deborah T. Chaleff

    发明人： Mark H. Pausch ,  Bradley A. Ozenberger ,  John R. Hadcock ,  Laura A. Price ,  Eileen M. Kajkowski ,  Donald R. Kirsch ,  Deborah T. Chaleff

    IPC分类号： C12N15/09 ,  C07K14/72 ,  C12N1/15 ,  C12N1/19 ,  C12N15/12 ,  C12P21/02 ,  C12Q1/02 ,  C12R1/865 ,  C12N15/63 ,  C07K14/705

    CPC分类号： C07K14/723 ,  C07K2319/00

    摘要： The present invention is directed to a expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein .alpha..beta..gamma. complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on the cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric G.alpha. protein is expressed in conjunction with nucleotide sequences from the yeast G protein .beta..gamma. subunits. A second aspect of the present invention provides expression vectors and transformed yeast cells encoding chimeric yeast/heterologous G protein coupled receptors. A third aspect of the present invention is directed to methods of assaying compounds using these expression constructs and yeast cell expression systems to determine the effects of ligand binding to the heterologous receptors expressed in the systems.

    摘要翻译： 本发明涉及转化的表达载体和酵母细胞，其含有编码G蛋白偶联受体（例如生长抑素受体）的第一异源核苷酸序列，以及编码全部或一部分的第二核苷酸序列 一个G蛋白αβγ复合物。 所述异源蛋白质在适当取向的宿主细胞膜中物理表达，用于配体的立体选择性结合，以及与细胞膜细胞质侧上的G蛋白的功能相互作用。 在一些实施方案中，编码异源或嵌合Gα蛋白的核苷酸序列与来自酵母G蛋白βγ亚基的核苷酸序列一起表达。 本发明的第二方面提供了编码嵌合酵母/异源G蛋白偶联受体的表达载体和转化的酵母细胞。 本发明的第三方面涉及使用这些表达构建体和酵母细胞表达系统测定化合物以确定配体与系统中表达的异源受体结合的作用的方法。

    cDNAs encoding proteins closely related to opioid receptors
    5.
    发明授权
    cDNAs encoding proteins closely related to opioid receptors 失效
    编码与阿片受体密切相关的蛋白质的cDNA

    公开(公告)号：US6046026A

    公开(公告)日：2000-04-04

    申请号：US676351

    申请日：1996-09-12

    申请人： Cecil Mark Eppler ,  Bradley A. Ozenberger ,  Jeffrey D. Hulmes

    发明人： Cecil Mark Eppler ,  Bradley A. Ozenberger ,  Jeffrey D. Hulmes

    IPC分类号： C07K14/705 ,  C12N15/12

    CPC分类号： C07K14/705

    摘要： Disclosed are novel DNAs encoding polypeptides related to opioid receptors, methods for expressing and isolating such polypeptides, and methods of use thereof. Also disclosed are vectors for expressing the novel DNAs of the invention.

    摘要翻译： PCT No.PCT / US95 / 00939 Sec。 371日期：1996年9月12日 102（e）日期1996年9月12日PCT 1995年1月20日PCT PCT。 WO95 / 19986 PCT公开号 日期1995年7月27日公开是编码与阿片受体相关的多肽的新型DNA，用于表达和分离这些多肽的方法及其使用方法。 还公开了用于表达本发明的新型DNA的载体。

    Methods of modulating apoptosis using inhibitors of brain-localized protein kinases homologous to homeodomain-interacting protein kinases
    7.
    发明授权
    Methods of modulating apoptosis using inhibitors of brain-localized protein kinases homologous to homeodomain-interacting protein kinases 失效
    使用与同源结构域相互作用蛋白激酶同源的脑定位蛋白激酶抑制剂调节凋亡的方法

    公开(公告)号：US07745164B2

    公开(公告)日：2010-06-29

    申请号：US11645056

    申请日：2006-12-26

    申请人： Wei Liu ,  Bradley A. Ozenberger ,  Leeying Wu ,  Ching-Hsiung Frederick Lo ,  Steven A. Haney ,  Hemchand Sookdeo ,  Jee Hyung Lee

    发明人： Wei Liu ,  Bradley A. Ozenberger ,  Leeying Wu ,  Ching-Hsiung Frederick Lo ,  Steven A. Haney ,  Hemchand Sookdeo ,  Jee Hyung Lee

    IPC分类号： C12Q1/48

    CPC分类号： C12N9/1205

    摘要： The invention provides isolated protein kinase polypeptides related to novel brain-localized protein kinases homologous to known homeodomain-interacting protein kinases (HIPKs), isolated nucleic acid molecules that encode these polypeptides, inhibitors, and methods related thereto. The novel polypeptides and nucleic acid molecules of the invention are termed HIPK4. The invention also provides genetically engineered expression vectors, host cells, and transgenic animals comprising the novel nucleic acid molecules of the invention. The invention additionally provides antisense and RNAi molecules to the nucleic acid molecules of the invention, as well as inhibitors, activators, and antibodies capable of binding to the protein kinase polypeptides of the invention. The invention further provides uses of HIPK4 and its inhibitors, e.g., mutants of HIPK4 lacking protein kinase activity, for modulation of apoptosis, as well as the prevention and treatment of neurological disorders and cancers.

    摘要翻译： 本发明提供与与已知同源结构域相互作用蛋白激酶（HIPK）同源的新型脑定位蛋白激酶相关的分离的蛋白激酶多肽，编码这些多肽的分离的核酸分子，抑制剂及其相关方法。 本发明的新型多肽和核酸分子称为HIPK4。 本发明还提供了遗传工程改造的表达载体，宿主细胞和包含本发明的新型核酸分子的转基因动物。 本发明还向本发明的核酸分子以及能够结合本发明的蛋白激酶多肽的抑制剂，激活剂和抗体提供反义和RNAi分子。 本发明还提供了HIPK4及其抑制剂（例如缺乏蛋白激酶活性的HIPK4的突变体）用于调节凋亡以及预防和治疗神经障碍和癌症的用途。

    Cell systems having specific interaction of peptide binding pairs
    8.
    发明授权
    Cell systems having specific interaction of peptide binding pairs 失效
    具有肽结合对的特异性相互作用的细胞系统

    公开(公告)号：US06284519B1

    公开(公告)日：2001-09-04

    申请号：US09305483

    申请日：1999-05-06

    申请人： Kathleen H. Young ,  Bradley A. Ozenberger

    发明人： Kathleen H. Young ,  Bradley A. Ozenberger

    IPC分类号： C12N119

    CPC分类号： C07K14/72 ,  C07K14/61 ,  C07K2319/00 ,  C12Q1/6897

    摘要： This invention relates to novel modified host cells which express heterologous fused proteins and methods of screening for test samples having peptide-binding activity; wherein the modified host cell comprises: (a) a gene sequence encoding a heterologous fusion protein; said fusion protein comprising a first peptide of a peptide binding pair, or segment of said first peptide, which is joined to either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation protein; (b) a gene sequence encoding a heterologous fusion protein, said fusion protein comprising a second peptide of the peptide binding pair in (a), or a segment thereof, fused to either a DNA binding domain or its corresponding transcriptional activation domain, whichever one is not employed in (a); (c) a reporter gene operatively associated with the transcriptional activation protein, or a portion thereof; (d) optionally, a deletion or mutation in the chromosomal DNA of the host cell for the transcriptional activation protein if present in the selected host cell.

    摘要翻译： 本发明涉及表达异源融合蛋白的新型修饰宿主细胞和筛选具有肽结合活性的测试样品的方法; 其中所述修饰的宿主细胞包含：（a）编码异源融合蛋白的基因序列; 所述融合蛋白包含肽结合对的第一肽或所述第一肽的片段，其连接到转录激活蛋白的DNA结合结构域或其相应的转录激活结构域; （b）编码异源融合蛋白的基因序列，所述融合蛋白包含（a）中的肽结合对的第二个肽或其片段，其融合到DNA结合结构域或其相应的转录激活结构域，无论哪个 （a）没有雇用; （c）与转录激活蛋白有效相关的报告基因，或其一部分; （d）任选地，如果存在于所选择的宿主细胞中，则转录激活蛋白的宿主细胞的染色体DNA中的缺失或突变。

    Cell systems having specific interaction of peptide binding pairs
    9.
    发明授权
    Cell systems having specific interaction of peptide binding pairs 失效
    具有肽结合对的特异性相互作用的细胞系统

    公开(公告)号：US06673540B1

    公开(公告)日：2004-01-06

    申请号：US09714258

    申请日：2000-11-17

    申请人： Kathleen H. Young ,  Bradley A. Ozenberger

    发明人： Kathleen H. Young ,  Bradley A. Ozenberger

    IPC分类号： C12Q168

    CPC分类号： C07K14/72 ,  C07K14/61 ,  C07K2319/00 ,  C12Q1/6897

    摘要： This invention relates to modified host cells which express heterologous fused proteins and methods of screening for test samples having peptide binding activity; wherein the modified host cell comprises: (a) a gene sequence encoding a heterologous fusion protein; the fusion protein comprising a first peptide of a peptide binding pair, or segment of the first peptide, which is joined to either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation protein; (b) a gene sequence encoding a heterologous fusion protein, the fusion protein comprising a second peptide of the peptide binding pair in (a), or a segment thereof, fused to either a DNA binding domain or its corresponding transcriptional activation domain, whichever one is not employed in (a); (c) a reporter gene operatively associated with the transcriptional activation protein, or a portion thereof; (d) optionally, a deletion or mutation in the chromosomal DNA of the host cell for the transcriptional activation protein if present in the selected host cell.

    摘要翻译： 本发明涉及表达异源融合蛋白的修饰的宿主细胞和筛选具有肽结合活性的测试样品的方法; 其中所述修饰的宿主细胞包含：（a）编码异源融合蛋白的基因序列; 所述融合蛋白包含肽结合对的第一肽或第一肽的片段，其连接到转录激活蛋白的DNA结合结构域或其相应的转录激活结构域; （b）编码异源融合蛋白的基因序列，所述融合蛋白包含（a）中的肽结合对的第二个肽或其部分融合到DNA结合结构域或其相应的转录激活结构域，无论哪个 （a）没有雇用; （c）与转录激活蛋白有效相关的报告基因，或其一部分; （d）任选地，如果存在于所选择的宿主细胞中，则转录激活蛋白的宿主细胞的染色体DNA中的缺失或突变。

    Cell systems having specific interaction of peptide binding pairs
    10.
    发明授权
    Cell systems having specific interaction of peptide binding pairs 失效

    公开(公告)号：US06251602B1

    公开(公告)日：2001-06-26

    申请号：US09263944

    申请日：1999-03-08

    申请人： Kathleen H. Young ,  Bradley A. Ozenberger

    发明人： Kathleen H. Young ,  Bradley A. Ozenberger

    IPC分类号： C12Q168

    CPC分类号： C07K14/72 ,  C07K14/61 ,  C07K2319/00 ,  C12Q1/6897

    摘要： This invention relates to novel modified host cells which express heterologous fused proteins and methods of screening for test samples having peptide-binding activity; wherein the modified host cell comprises: (a) a gene sequence encoding a heterologous fusion protein; said fusion protein comprising a first peptide of a peptide binding pair, or segment of said first peptide, which is joined to either a DNA binding domain or its corresponding transcriptional activation domain of a transcriptional activation protein; (b) a gene sequence encoding a heterologous fusion protein, said fusion protein comprising a second peptide of the peptide binding pair in (a), or a segment thereof, fused to either a DNA binding domain or its corresponding transcriptional activation domain, whichever one is not employed in (a); (c) a reporter gene operatively associated with the transcriptional activation protein, or a portion thereof; (d) optionally, a deletion or mutation in the chromosomal DNA of the host cell for the transcriptional activation protein if present in the selected host cell.