公开(公告)号：US20060069518A1

公开(公告)日：2006-03-30

申请号：US11226882

申请日：2005-09-14

申请人： Lex Cowsert ,  Brenda Baker ,  John McNeil ,  Susan Freier ,  Henri Sasmor ,  Douglas Brooks ,  Cara Ohashi ,  Jacqueline Wyatt ,  Alexander Borchers ,  Timothy Vickers

发明人： Lex Cowsert ,  Brenda Baker ,  John McNeil ,  Susan Freier ,  Henri Sasmor ,  Douglas Brooks ,  Cara Ohashi ,  Jacqueline Wyatt ,  Alexander Borchers ,  Timothy Vickers

IPC分类号： C40B30/02

CPC分类号： C12Q1/6811 ,  B01J2219/007 ,  C12N15/1048 ,  G16B20/00 ,  G16B30/00 ,  G16B35/00 ,  G16C20/60

摘要： Interative, preferably computer based iterative processes for generating synthetic compounds with desired physical, chemical and/or bioactive properties, i.e., active compounds, are provided. During iterations of the processes, a target nucleic acid sequence is provided or selected, and a library of candidate nucleobase sequences is generated in silico according to defined criteria. A “virtual” oligonucleotide chemistry is chosen and a library of virtual oligonucleotide compounds having the selected nucleobase sequences is generated. These virtual compounds are reviewed and compounds predicted to have particular properties are selected. The selected compounds are robotically synthesized and are preferably robotically assayed for a desired physical, chemical or biological activity. Active compounds are thus generated and, at the same time, preferred sequences and regions of the target nucleic acid that are amenable to oligonucleotide or sequence-based modulation are identified.

摘要翻译： 提供了用于产生具有期望的物理，化学和/或生物活性的合成化合物（即活性化合物）的相互优选的基于计算机的迭代方法。 在该过程的迭代过程中，提供或选择靶核酸序列，并且根据确定的标准以计算机生成候选核碱基序列的文库。 选择“虚拟”寡核苷酸化学，并产生具有选定的核碱基序列的虚拟寡核苷酸化合物文库。 审查这些虚拟化合物，并选择预测具有特定性质的化合物。 所选择的化合物是机械合成的，并且优选机械地测定所需的物理，化学或生物活性。 因此产生活性化合物，并且同时确定适用于寡核苷酸或基于序列的调节的靶核酸的优选序列和区域。

公开(公告)号：US20050261228A1

公开(公告)日：2005-11-24

申请号：US11089191

申请日：2005-03-23

申请人： C. Bennett ,  Brenda Baker ,  Susan Freier ,  Susan Gregory ,  Ronnie Hanecak ,  Kevin Anderson ,  Ming-Yi Chiang ,  Vickie Brown-Driver ,  David Ecker ,  Timothy Vickers ,  Jacqueline Wyatt ,  Eric Marcusson ,  Nicholas Dean ,  Sanjay Bhanot ,  Elizabeth Ackermann ,  Lex Cowsert

发明人： C. Bennett ,  Brenda Baker ,  Susan Freier ,  Susan Gregory ,  Ronnie Hanecak ,  Kevin Anderson ,  Ming-Yi Chiang ,  Vickie Brown-Driver ,  David Ecker ,  Timothy Vickers ,  Jacqueline Wyatt ,  Eric Marcusson ,  Nicholas Dean ,  Sanjay Bhanot ,  Elizabeth Ackermann ,  Lex Cowsert

IPC分类号： A61K38/00 ,  A61K48/00 ,  C07H21/04 ,  C12N15/113 ,  C12N15/85

CPC分类号： C12N15/1136 ,  A61K38/00 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  Y02P20/582 ,  C12N2310/3525

摘要： Compounds, compositions and methods are provided for modulating the expression of Interleukin 12 p35 subunit. The compositions comprise chemically modified antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding Interleukin 12 p35 subunit. Methods of using these compounds for modulation of Interleukin 12 p35 subunit expression and for treatment of diseases associated with expression of Interleukin 12 p35 subunit are provided.

摘要翻译： 提供化合物，组合物和方法用于调节白细胞介素12p35亚基的表达。 组合物包含靶向编码白细胞介素12p35亚基的核酸的化学修饰的反义化合物，特别是反义寡核苷酸。 提供了使用这些化合物调节白介素12p35亚基表达和治疗与白细胞介素12p35亚基表达相关的疾病的方法。

公开(公告)号：US20050037387A1

公开(公告)日：2005-02-17

申请号：US10852630

申请日：2004-05-24

申请人： Donna Ward ,  Andrew Watt ,  Brenda Baker ,  Timothy Vickers ,  Kenneth Dobie ,  Bryan Kraynack

发明人： Donna Ward ,  Andrew Watt ,  Brenda Baker ,  Timothy Vickers ,  Kenneth Dobie ,  Bryan Kraynack

IPC分类号： C07H21/02 ,  C12N20060101 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/68

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/10 ,  C12N2320/50 ,  C12N2310/3525

摘要： Compounds, compositions and methods are provided for modulating the RNAi pathway. The compositions comprise oligonucleotides, targeted to nucleic acid molecules encoding EIF2C2. Methods of using these compounds for modulation of RNA interference as well as EIF2C2 expression and for diagnosis and treatment of disease associated with expression of EIF2C2 are provided. Also provided are compounds, compositions and methods for modulating the expression of DDX36. The compositions comprise oligonucleotides, targeted to nucleic acid encoding DDX36. Methods of using these compounds for modulation of DDX36 expression and for diagnosis and treatment of diseases and conditions associated with expression of DDX36 are provided.

摘要翻译： 提供化合物，组合物和方法用于调节RNAi途径。 组合物包含靶向编码EIF2C2的核酸分子的寡核苷酸。 提供了使用这些化合物调节RNA干扰以及EIF2C2表达以及用于诊断和治疗与EIF2C2表达相关的疾病的方法。 还提供了用于调节DDX36表达的化合物，组合物和方法。 组合物包含针对编码DDX36的核酸的寡核苷酸。 提供了使用这些化合物调制DDX36表达和用于诊断和治疗与DDX36表达相关的疾病和病症的方法。

公开(公告)号：US20050261218A1

公开(公告)日：2005-11-24

申请号：US10909125

申请日：2004-07-30

申请人： Christine Esau ,  Bridget Lollo ,  C. Bennett ,  Susan Freier ,  Richard Griffey ,  Brenda Baker ,  Timothy Vickers ,  Eric Marcusson ,  Erich Koller ,  Eric Swayze ,  Ravi Jain ,  Balkrishen Bhat ,  Eigen Peralta

发明人： Christine Esau ,  Bridget Lollo ,  C. Bennett ,  Susan Freier ,  Richard Griffey ,  Brenda Baker ,  Timothy Vickers ,  Eric Marcusson ,  Erich Koller ,  Eric Swayze ,  Ravi Jain ,  Balkrishen Bhat ,  Eigen Peralta

IPC分类号： A61K20060101 ,  A61K48/00 ,  C07H21/02 ,  C12N15/11 ,  C12N15/113

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/113 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/31 ,  C12N2310/315 ,  C12N2310/317 ,  C12N2310/3181 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2310/351 ,  C12N2310/3515 ,  C12N2320/11 ,  C12N2320/30 ,  C12N2320/50 ,  C12N2310/3525

摘要： Compounds, compositions and methods are provided for modulating the expression and function of small non-coding RNAs. The compositions comprise oligomeric compounds, targeted to small non-coding RNAs. Methods of using these compounds for modulation of small non-coding RNAs as well as downstream targets of these RNAs and for diagnosis and treatment of disease associated with small non-coding RNAs are also provided.

摘要翻译： 提供化合物，组合物和方法用于调节小非编码RNA的表达和功能。 组合物包含靶向小的非编码RNA的寡聚化合物。 还提供了使用这些化合物调节小非编码RNA以及这些RNA的下游靶标以及用于诊断和治疗与小非编码RNA相关的疾病的方法。

公开(公告)号：US20050048495A1

公开(公告)日：2005-03-03

申请号：US10651772

申请日：2003-08-29

申请人： Brenda Baker ,  Timothy Vickers

发明人： Brenda Baker ,  Timothy Vickers

IPC分类号： C07H21/04 ,  C12Q1/68 ,  A61K48/00

CPC分类号： C07H21/04

摘要： Compounds, compositions and methods are provided for modulating the expression of splice variants or products. The compositions comprise oligonucleotides, targeted to nucleic acid encoding splice variants or products. Methods of using these compounds for modulation of expression of splice variants or products and for diagnosis and treatment of disease associated with expression of splice variants or products are provided.

摘要翻译： 提供化合物，组合物和方法用于调节剪接变体或产物的表达。 组合物包含针对核酸编码剪接变体或产物的寡核苷酸。 提供了使用这些化合物调节剪接变体或产物的表达以及用于诊断和治疗与剪接变体或产物的表达相关的疾病的方法。

公开(公告)号：US20050033524A1

公开(公告)日：2005-02-10

申请号：US10649467

申请日：2003-08-27

申请人： Lex Cowsert ,  Brenda Baker ,  John McNeil ,  Susan Freier ,  Henri Sasmor ,  Douglas Brooks ,  Cara Ohashi ,  Jacqueline Wyatt ,  Alexander Borchers ,  Timothy Vickers

发明人： Lex Cowsert ,  Brenda Baker ,  John McNeil ,  Susan Freier ,  Henri Sasmor ,  Douglas Brooks ,  Cara Ohashi ,  Jacqueline Wyatt ,  Alexander Borchers ,  Timothy Vickers

IPC分类号： C12N15/09 ,  C07B61/00 ,  C12N15/10 ,  C12Q1/68 ,  C12Q1/6811 ,  C40B30/02 ,  C40B40/02 ,  C40B50/02 ,  C40B50/06 ,  G06F17/30 ,  G06F17/50 ,  G06F19/18 ,  G06F19/22 ,  G06F19/00 ,  G01N33/48 ,  G01N33/50 ,  C07H21/04

CPC分类号： C12Q1/6811 ,  B01J2219/007 ,  C12N15/1048 ,  G16B20/00 ,  G16B30/00 ,  G16B35/00 ,  G16C20/60

摘要： Interative, preferably computer based iterative processes for generating synthetic compounds with desired physical, chemical and/or bioactive properties, i.e., active compounds, are provided. During iterations of the processes, a target nucleic acid sequence is provided or selected, and a library of candidate nucleobase sequences is generated in silico according to defined criteria. A “virtual” oligonucleotide chemistry is chosen and a library of virtual oligonucleotide compounds having the selected nucleobase sequences is generated. These virtual compounds are reviewed and compounds predicted to have particular properties are selected. The selected compounds are robotically synthesized and are preferably robotically assayed for a desired physical, chemical or biological activity. Active compounds are thus generated and, at the same time, preferred sequences and regions of the target nucleic acid that are amenable to oligonucleotide or sequence-based modulation are identified.

摘要翻译： 提供了用于产生具有期望的物理，化学和/或生物活性的合成化合物（即活性化合物）的相互优选的基于计算机的迭代方法。 在该过程的迭代过程中，提供或选择靶核酸序列，并且根据确定的标准以计算机生成候选核碱基序列的文库。 选择“虚拟”寡核苷酸化学，并产生具有选定的核碱基序列的虚拟寡核苷酸化合物文库。 审查这些虚拟化合物，并选择预测具有特定性质的化合物。 所选择的化合物是机械合成的，并且优选机械地测定所需的物理，化学或生物活性。 因此产生活性化合物，并且同时确定适用于寡核苷酸或基于序列的调节的靶核酸的优选序列和区域。

公开(公告)号：US20090298910A1

公开(公告)日：2009-12-03

申请号：US11721326

申请日：2005-12-12

申请人： Richard H. Griffey ,  Timothy Vickers ,  Ranjan Perera

发明人： Richard H. Griffey ,  Timothy Vickers ,  Ranjan Perera

IPC分类号： A61K31/7088 ,  G01N33/00 ,  G01N33/566 ,  C12Q1/68 ,  C07H21/04 ,  C12N5/06

CPC分类号： C12N15/113 ,  C12N15/11 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/11 ,  C12N2320/12 ,  C12N2330/10 ,  Y10T436/143333 ,  C12N2310/3525

摘要： Methods are provided for the identification of compounds that selectively modulate epigenetic changes in gene expression. Compounds, compositions, kits or assays devices, and methods are provided for modulating the expression, endogenous levels or the function of small non-coding RNAs cognate to or transcribed by heterochromatic regions subject to epigenetic regulation (i.e., promoters, enhancers, centromeres, telomeres, origins of DNA replication, imprinted loci, or loci marked by dosage-compensation), and for modulating the formation or function of heterochromatin in cells, tissues or animals.

摘要翻译： 提供了用于鉴定选择性调节基因表达的表观遗传变化的化合物的方法。 提供化合物，组合物，试剂盒或测定装置和方法用于调节受表观遗传调节的异染色体区域（即启动子，增强子，着丝粒，端粒）相关或转录的小非编码RNA的表达，内源水平或功能 ，DNA复制起点，印迹位点或由剂量补偿标记的基因座），并调节细胞，组织或动物中异染色质的形成或功能。

公开(公告)号：US5698391A

公开(公告)日：1997-12-16

申请号：US357396

申请日：1994-12-16

申请人： Phillip Dan Cook ,  David J. Ecker ,  Jacqueline Wyatt ,  Thomas W. Bruice ,  Kevin Anderson ,  Ronnie Hanecak ,  Timothy Vickers ,  Peter Davis ,  Susan M. Freier ,  Yogesh S. Sanghvi ,  Vickie Brown-Driver

发明人： Phillip Dan Cook ,  David J. Ecker ,  Jacqueline Wyatt ,  Thomas W. Bruice ,  Kevin Anderson ,  Ronnie Hanecak ,  Timothy Vickers ,  Peter Davis ,  Susan M. Freier ,  Yogesh S. Sanghvi ,  Vickie Brown-Driver

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12Q1/70

CPC分类号： C12Q1/6811 ,  G01N33/5308

摘要： Methods useful for the determination of oligomers which have specific activity for a target molecule from a pool of primarily randomly assembled oligomers are provided. The disclosed methods involve repeated syntheses of increasingly simplified sets of oligomers coupled with selection procedures for determining oligomers having the highest activity. Freedom from the use of enzymes allows the application of these methods to any molecules which can be oligomerized in a controlled fashion.

摘要翻译： 提供了用于测定从主要随机组装的低聚物库中的靶分子具有比活性的低聚物的方法。 所公开的方法涉及重复合成越来越简单的低聚体组合，以及用于确定具有最高活性的寡聚体的选择程序。 不使用酶允许将这些方法应用于可以以受控方式低聚的任何分子。

公开(公告)号：US08093061B2

公开(公告)日：2012-01-10

申请号：US12445854

申请日：2007-10-18

申请人： Walter F Lima ,  Timothy Vickers ,  Stanley T Crooke

发明人： Walter F Lima ,  Timothy Vickers ,  Stanley T Crooke

IPC分类号： G01N33/53 ,  G01N33/00

CPC分类号： C12Q1/6876 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2320/10 ,  Y10T436/143333

摘要： Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2.

公开(公告)号：US07879992B2

公开(公告)日：2011-02-01

申请号：US11339785

申请日：2006-01-24

申请人： Timothy Vickers ,  Nicholas M. Dean

发明人： Timothy Vickers ,  Nicholas M. Dean

IPC分类号： C07H21/04

CPC分类号： C12N15/113 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/341 ,  C12N2310/345 ,  C12N2310/3521

摘要： Antisense compounds, compositions and methods are provided for modulating the expression of MyD88. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding MyD88. Methods of using these compounds for modulation of MyD88 expression and for treatment of diseases associated with expression of MyD88 are provided.

摘要翻译： 提供反义化合物，组合物和方法用于调节MyD88的表达。 组合物包含靶向编码MyD88的核酸的反义化合物，特别是反义寡核苷酸。 提供了使用这些化合物调节MyD88表达和治疗MyD88表达相关疾病的方法。