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公开(公告)号:US08535927B1
公开(公告)日:2013-09-17
申请号:US10576331
申请日:2004-11-19
申请人: Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Eugene S. Sadlowski , Andrew Shaw , Leo van Marrewijk , Wilhelmus A. H. Van Der Kleij
发明人: Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Eugene S. Sadlowski , Andrew Shaw , Leo van Marrewijk , Wilhelmus A. H. Van Der Kleij
CPC分类号: C12N9/52 , C07K2299/00 , C11D3/386
摘要: The present invention provides novel serine proteases, novel genetic material encoding these enzymes, and proteolytic proteins obtained from Micrococcineae spp., including but not limited to Cellulomonas spp. and variant proteins developed therefrom. In particular, the present invention provides protease compositions obtained from a Cellulomonas spp, DNA encoding the protease, vectors comprising the DNA encoding the protease, host cells transformed with the vector DNA, and an enzyme produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising protease(s) obtained from a Micrococcineae spp., including but not limited to Cellulomonas spp. In alternative embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These mutant proteases also find use in numerous applications.
摘要翻译: 本发明提供了新的丝氨酸蛋白酶,编码这些酶的新型遗传物质,以及从微球菌属(Micrococcineae spp。)获得的蛋白水解蛋白,包括但不限于纤维单胞菌属(Cellulomonas spp) 和从其开发的变体蛋白质。 特别地,本发明提供了从纤维单胞菌属(Splulomonas spp)获得的蛋白酶组合物,编码蛋白酶的DNA,包含编码蛋白酶的载体的载体,用载体DNA转化的宿主细胞和由宿主细胞产生的酶。 本发明还提供了清洁组合物(例如洗涤剂组合物),动物饲料组合物,以及包含从微球菌(Micrococcineae spp。)获得的蛋白酶的纺织和皮革加工组合物,其包括但不限于纤维单胞菌属(Cellulomonas spp) 在替代实施方案中,本发明提供衍生自本文所述的野生型蛋白酶的突变体(即,变体)蛋白酶。 这些突变型蛋白酶也可用于许多应用。
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公开(公告)号:US07985569B2
公开(公告)日:2011-07-26
申请号:US11809104
申请日:2007-05-31
申请人: Wolfgang Aehle , David A. Estell , Ronaldus W. J. Hommes , Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Andrew Shaw , Wilhelmus A. H. van der Kley , Leonardus P. M. van Marrewijk
发明人: Wolfgang Aehle , David A. Estell , Ronaldus W. J. Hommes , Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Andrew Shaw , Wilhelmus A. H. van der Kley , Leonardus P. M. van Marrewijk
IPC分类号: C12N9/48
CPC分类号: C12N9/52 , A23K20/189 , A23K50/75 , C11D3/386 , C12N9/96 , C12Y304/21 , D06M16/003
摘要: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.
摘要翻译: 本发明提供了具有多个取代的新型微球菌spp丝氨酸蛋白酶。 特别地,本发明提供了具有多个取代的丝氨酸蛋白酶,编码这些蛋白酶的DNA,包含编码蛋白酶的DNA的载体,用载体DNA转化的宿主细胞和由宿主细胞产生的酶。 本发明还提供了清洁组合物(例如洗涤剂组合物),动物饲料组合物以及包含这些丝氨酸蛋白酶变体的纺织和皮革加工组合物。 在特别优选的实施方案中,本发明提供衍生自本文所述的野生型蛋白酶的突变体(即,变体)蛋白酶。 这些变体蛋白酶也可用于许多应用。
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公开(公告)号:US20110251116A1
公开(公告)日:2011-10-13
申请号:US13162104
申请日:2011-06-16
申请人: Wolfgang Aehle , David A. Estell , Ronaldus W.J. Hommes , Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Andrew Shaw , Wilhelmus A.H. Van der Kley , Leonardus P.M. Van Marrewijk
发明人: Wolfgang Aehle , David A. Estell , Ronaldus W.J. Hommes , Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Andrew Shaw , Wilhelmus A.H. Van der Kley , Leonardus P.M. Van Marrewijk
CPC分类号: C12N9/52 , A23K20/189 , A23K50/75 , C11D3/386 , C12N9/96 , C12Y304/21 , D06M16/003
摘要: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.
摘要翻译: 本发明提供了具有多个取代的新型微球菌spp丝氨酸蛋白酶。 特别地,本发明提供了具有多个取代的丝氨酸蛋白酶,编码这些蛋白酶的DNA,包含编码蛋白酶的DNA的载体,用载体DNA转化的宿主细胞和由宿主细胞产生的酶。 本发明还提供了清洁组合物(例如洗涤剂组合物),动物饲料组合物以及包含这些丝氨酸蛋白酶变体的纺织和皮革加工组合物。 在特别优选的实施方案中,本发明提供衍生自本文所述的野生型蛋白酶的突变体(即,变体)蛋白酶。 这些变体蛋白酶也可用于许多应用。
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公开(公告)号:US08455234B2
公开(公告)日:2013-06-04
申请号:US13162104
申请日:2011-06-16
申请人: Wolfgang Aehle , David A. Estell , Ronaldus W. J. Hommes , Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Andrew Shaw , Wilhelmus A. H. van der Kley , Leonardus P. M. van Marrewijk
发明人: Wolfgang Aehle , David A. Estell , Ronaldus W. J. Hommes , Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Andrew Shaw , Wilhelmus A. H. van der Kley , Leonardus P. M. van Marrewijk
IPC分类号: C12N9/48
CPC分类号: C12N9/52 , A23K20/189 , A23K50/75 , C11D3/386 , C12N9/96 , C12Y304/21 , D06M16/003
摘要: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.
摘要翻译: 本发明提供了具有多个取代的新型微球菌spp丝氨酸蛋白酶。 特别地,本发明提供了具有多个取代的丝氨酸蛋白酶,编码这些蛋白酶的DNA,包含编码蛋白酶的DNA的载体,用载体DNA转化的宿主细胞和由宿主细胞产生的酶。 本发明还提供了清洁组合物(例如洗涤剂组合物),动物饲料组合物以及包含这些丝氨酸蛋白酶变体的纺织和皮革加工组合物。 在特别优选的实施方案中,本发明提供衍生自本文所述的野生型蛋白酶的突变体(即,变体)蛋白酶。 这些变体蛋白酶也可用于许多应用。
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公开(公告)号:US20080063774A1
公开(公告)日:2008-03-13
申请号:US11809104
申请日:2007-05-31
申请人: Wolfgang Aehle , David Estell , Ronaldus Hommes , Brian Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran Poulose , Andrew Shaw , Wilhelmus Van Der Kley , Leonardus Van Marrewijk
发明人: Wolfgang Aehle , David Estell , Ronaldus Hommes , Brian Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran Poulose , Andrew Shaw , Wilhelmus Van Der Kley , Leonardus Van Marrewijk
IPC分类号: A23K1/00 , C07H21/00 , C11D7/42 , C12N1/00 , C12N1/15 , C12N1/21 , C12N15/63 , C12N9/50 , D06M16/00
CPC分类号: C12N9/52 , A23K20/189 , A23K50/75 , C11D3/386 , C12N9/96 , C12Y304/21 , D06M16/003
摘要: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.
摘要翻译: 本发明提供了具有多个取代的新型微球菌spp丝氨酸蛋白酶。 特别地,本发明提供了具有多个取代的丝氨酸蛋白酶,编码这些蛋白酶的DNA,包含编码蛋白酶的DNA的载体,用载体DNA转化的宿主细胞和由宿主细胞产生的酶。 本发明还提供了清洁组合物(例如洗涤剂组合物),动物饲料组合物以及包含这些丝氨酸蛋白酶变体的纺织和皮革加工组合物。 在特别优选的实施方案中,本发明提供衍生自本文所述的野生型蛋白酶的突变体(即,变体)蛋白酶。 这些变体蛋白酶也可用于许多应用。
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公开(公告)号:US08772007B2
公开(公告)日:2014-07-08
申请号:US10581014
申请日:2004-12-03
申请人: Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brian E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
发明人: Neelam S. Amin , Matthew G. Boston , Richard R. Bott , Marguerite A. Cervin , Edward M. Concar , Marc E. Gustwiller , Brian E. Jones , Klaus Liebeton , Gregory S. Miracle , Hiroshi Oh , Ayrookaran J. Poulose , Sandra W. Ramer , Jeffrey J. Scheibel , Walter Weyler , Gregory M. Whited
CPC分类号: A01N63/00 , C11D3/38636 , C12N9/16 , C12N9/50 , C12N9/52
摘要: The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.
摘要翻译: 本发明提供包含至少一种用于清洁和其它应用的过水解酶的方法和组合物。 在一些特别优选的实施方案中,本发明提供了用于产生过酸的方法和组合物。 本发明特别用于涉及清洗,漂白和消毒的应用中。
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公开(公告)号:US08574884B2
公开(公告)日:2013-11-05
申请号:US13345547
申请日:2012-01-06
申请人: Andrew Shaw , Louise Wallace , David A. Estell , Ronaldus W. J. Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
发明人: Andrew Shaw , Louise Wallace , David A. Estell , Ronaldus W. J. Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
IPC分类号: C12N9/54 , C12N9/50 , C12N1/20 , C12N15/00 , C12P21/06 , D06M16/00 , C02F3/34 , C14C1/00 , C12P1/00 , C07H21/04 , C11D3/00 , C07K1/00
CPC分类号: C12N9/54 , C11D1/02 , C11D3/386 , C11D3/38663 , C12Y304/24
摘要: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
摘要翻译: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中,中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中,本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中,中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中,中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中,中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁,漂白和消毒的应用。
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公开(公告)号:US20090263882A1
公开(公告)日:2009-10-22
申请号:US12473735
申请日:2009-05-28
申请人: Andrew Shaw , Louise Wallace , David A. Estell , Ronaldus W. J, Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
发明人: Andrew Shaw , Louise Wallace , David A. Estell , Ronaldus W. J, Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
IPC分类号: C12N9/50
CPC分类号: C12N9/54 , C11D1/02 , C11D3/386 , C11D3/38663 , C12Y304/24
摘要: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
摘要翻译: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中,中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中,本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中,中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中,中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中,中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁,漂白和消毒的应用。
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公开(公告)号:US20120107907A1
公开(公告)日:2012-05-03
申请号:US13345547
申请日:2012-01-06
申请人: Andrew Shaw , Louise Wallace , David A. Estell , Ronald W. J. Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
发明人: Andrew Shaw , Louise Wallace , David A. Estell , Ronald W. J. Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
CPC分类号: C12N9/54 , C11D1/02 , C11D3/386 , C11D3/38663 , C12Y304/24
摘要: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
摘要翻译: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中,中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中,本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中,中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中,中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中,中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁,漂白和消毒的应用。
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公开(公告)号:US08114656B2
公开(公告)日:2012-02-14
申请号:US12473735
申请日:2009-05-28
申请人: Andrew Shaw , Louise Wallace , David A. Estell , Ronaldus W. J. Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
发明人: Andrew Shaw , Louise Wallace , David A. Estell , Ronaldus W. J. Hommes , Sang-Kyu Lee , Hiroshi Oh , Eugene S. Sadlowski
CPC分类号: C12N9/54 , C11D1/02 , C11D3/386 , C11D3/38663 , C12Y304/24
摘要: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.
摘要翻译: 本发明提供包含至少一种具有改善的储存稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中,中性金属蛋白酶可用于清洁和其它应用。 在一些特别优选的实施方案中,本发明提供了包含从芽孢杆菌属芽孢杆菌获得的中性金属蛋白酶的方法和组合物。 在一些更特别优选的实施方案中,中性金属蛋白酶从解淀粉芽孢杆菌获得。 在另外的优选实施方案中,中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的变体。 在另外的实施方案中,中性金属蛋白酶是解淀粉芽孢杆菌中性金属蛋白酶的同系物。 本发明特别用于包括但不限于清洁,漂白和消毒的应用。
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