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1.发明授权Gene variants coding for proteins from the metabolic pathway of fine chemicals 有权编码精细化学物质代谢途径蛋白质的基因变体公开(公告)号：US07566557B2
公开(公告)日：2009-07-28
申请号：US10583404
申请日：2004-12-16
申请人： Corinna Klopprogge , Oskar Zelder , Burkhard Kröger , Hartwig Schröder , Stefan Haefner , Uwe Ruffer , Claudia Isabella Graef , Gregor Haberhauer
发明人： Corinna Klopprogge , Oskar Zelder , Burkhard Kröger , Hartwig Schröder , Stefan Haefner , Uwe Ruffer , Claudia Isabella Graef , Gregor Haberhauer
CPC分类号： C07K14/34
摘要： The present invention relates to mutated nucleic acids and proteins of the metabolic pathway of fine chemicals, to processes for preparing genetically modified producer organisms, to processes for preparing fine chemicals by culturing genetically modified organisms, and to genetically modified organisms themselves.
摘要翻译： 本发明涉及精细化学品的代谢途径的突变核酸和蛋白质，制备经遗传修饰的生物体生物体的方法，通过培养经遗传修饰的生物体来制备精细化学品的方法以及转基因生物本身。
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公开(公告)号：US20090246836A1
公开(公告)日：2009-10-01
申请号：US10582822
申请日：2004-12-15
摘要： The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
摘要翻译： 本发明涉及核酸序列用于调节基因的转录和表达，新型启动子和表达单元本身的用途，用于改变或引起基因的转录速率和/或表达速率的方法，包含表达单位的表达盒 具有改变或引起的转录速率和/或表达速率的转基因微生物，以及通过培养转基因微生物来制备生物合成产物的方法。
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公开(公告)号：US08728795B2
公开(公告)日：2014-05-20
申请号：US12038642
申请日：2008-02-27
摘要： The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
摘要翻译： 本发明涉及核酸序列用于调节基因的转录和表达，新型启动子和表达单元本身的用途，用于改变或引起基因的转录速率和/或表达速率的方法，包含表达单位的表达盒 具有改变或引起的转录速率和/或表达速率的转基因微生物，以及通过培养转基因微生物来制备生物合成产物的方法。
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公开(公告)号：US07785779B2
公开(公告)日：2010-08-31
申请号：US10582918
申请日：2004-12-15
摘要： The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.
摘要翻译： 本发明涉及核酸序列用于调节基因的转录和表达，新型启动子和表达单元本身的用途，用于改变或引起基因的转录速率和/或表达速率的方法，包含表达单位的表达盒 具有改变或引起的转录速率和/或表达速率的转基因微生物，以及通过培养转基因微生物来制备生物合成产物的方法。
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5.发明授权公开(公告)号：US08048651B2
公开(公告)日：2011-11-01
申请号：US12533677
申请日：2009-07-31
申请人： Oskar Zelder , Corinna Klopprogge , Hartwig Schroder , Stefan Haefner , Burkhard Kröger , Patrick Kiefer , Elmar Heinzle , Christoph Wittmann
发明人： Oskar Zelder , Corinna Klopprogge , Hartwig Schroder , Stefan Haefner , Burkhard Kröger , Patrick Kiefer , Elmar Heinzle , Christoph Wittmann
IPC分类号： C12P13/06 , C12P13/08 , C12P13/10 , C12P13/12 , C12P23/00 , C12N9/16 , C12N15/70 , C12N1/20 , C12N15/74 , C07H21/04
摘要： The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.
摘要翻译： 本发明的特征在于通过使酶编码基因（即果糖-1,6-二磷酸酶）失调来增加精细化学品例如来自微生物例如棒状杆菌的赖氨酸的生产方法。 在优选的实施方案中，本发明提供了通过增加果糖-1,6-二磷酸酶活性的表达来增加谷氨酸棒杆菌中赖氨酸的产生的方法。 本发明还提供了通过调节向草酰乙酸（OAA）的碳通量来生产赖氨酸的新方法。 在优选的实施方案中，本发明提供了通过使用果糖或蔗糖作为碳源来生产赖氨酸的方法。
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6.发明申请Coryneform Bacteria with Formate-THF-Synthetase and/or Glycine Cleavage Activity 审中-公开具有甲酸-THF合成酶和/或甘氨酸裂解活性的棒状杆菌细菌公开(公告)号：US20100003727A1
公开(公告)日：2010-01-07
申请号：US12519909
申请日：2008-02-14
申请人： Oskar Zelder , Hartwig Schröder , Corinna Klopprogge , Andrea Herold , Stefan Haefner , Thomas A. Patterson , R. Rogers Yocum , Janice G. Pero
发明人： Oskar Zelder , Hartwig Schröder , Corinna Klopprogge , Andrea Herold , Stefan Haefner , Thomas A. Patterson , R. Rogers Yocum , Janice G. Pero
摘要： The present invention relates to microorganisms, in particular C. glutamicum in which the formation of N5,N10-methylene-THF is increased.The present invention also relates to the use of such microorganisms for producing methionine.
摘要翻译： 本发明涉及增加N5，N10-亚甲基-THF的形成的微生物，特别是谷氨酸棒杆菌。 本发明还涉及这种微生物用于产生甲硫氨酸的用途。
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7.发明申请Nucleic acid encoding a cobalamin-dependent methionine synthase polypeptide 审中-公开编码钴胺素依赖性甲硫氨酸合成酶多肽的核酸公开(公告)号：US20120295315A1
公开(公告)日：2012-11-22
申请号：US13524184
申请日：2012-06-15
申请人： Oskar Zelder , Wolfgang Grabarse , Corinna Klopprogge , Hartwig Schröder , Stefan Haefner , Anja Knietsch , Andrea Herold
发明人： Oskar Zelder , Wolfgang Grabarse , Corinna Klopprogge , Hartwig Schröder , Stefan Haefner , Anja Knietsch , Andrea Herold
CPC分类号： C12N9/0091 , C12P13/12
摘要： The present invention relates to nulceotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof being modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of such nucleotide sequences.
摘要翻译： 本发明涉及编码酶促活性钴胺素 - 甲硫氨酸合成酶的核糖核酸序列及其功能片段，其与相应的野生型酶相比被修饰，使得所述酶显示产品抑制蛋氨酸的产物减少。 本发明还涉及由该核苷酸序列编码的多肽和包含该核苷酸序列的宿主细胞。 此外，本发明涉及通过利用这种核苷酸序列在宿主生物体中产生甲硫氨酸的方法。
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8.发明授权Microorganisms with a reactivation system for cob(I)alamin-dependent methionine synthase 失效具有cob（I）氨基酸依赖性甲硫氨酸合酶活化系统的微生物公开(公告)号：US08163532B2
公开(公告)日：2012-04-24
申请号：US12472432
申请日：2009-05-27
申请人： Oskar Zelder , Hartwig Schröder , Corinna Klopprogge , Andrea Herold , Stefan Haefner , R. Rogers Yocum , Thomas A. Patterson , Mark Williams
发明人： Oskar Zelder , Hartwig Schröder , Corinna Klopprogge , Andrea Herold , Stefan Haefner , R. Rogers Yocum , Thomas A. Patterson , Mark Williams
CPC分类号： C12P13/12 , C12N9/1007
摘要： The present invention relates to microorganisms and methods for producing methionine by reactivation of the MetH enzyme.
摘要翻译： 本发明涉及通过MetH酶的再活化来生产甲硫氨酸的微生物和方法。
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9.发明申请Production Process for Fine Chemicals Using Microorganisms with Reduced Isocitrate Dehydrogenase Activity 审中-公开使用具有降低的异柠檬酸脱氢酶活性的微生物的精细化学品的生产方法公开(公告)号：US20110207183A1
公开(公告)日：2011-08-25
申请号：US12990240
申请日：2009-04-28
申请人： Andrea Herold , Hartwig Schröder , Weol Kyu Jeong , Corinna Klopprogge , Oskar Zelder , Stefan Haefner , Ulrike Richter , Judith Becker , Christoph Wittmann
发明人： Andrea Herold , Hartwig Schröder , Weol Kyu Jeong , Corinna Klopprogge , Oskar Zelder , Stefan Haefner , Ulrike Richter , Judith Becker , Christoph Wittmann
IPC分类号： C12P13/20 , C12P1/00 , C12P1/04 , C12P13/04 , C12P13/00 , C12P13/08 , C12N1/00 , C12N1/21 , C12P13/02 , C12P17/12 , C12P17/10 , C12P13/12 , C12P7/40
CPC分类号： C12P13/001 , C12N9/0006 , C12P13/04 , C12P13/08 , C12P17/12 , C12Y101/01041
摘要： The present invention is directed to a method utilizing a microorganism with reduced isocitrate dehydrogenase activity for the production of fine chemicals. Said fine chemicals may be amino acids, monomers for polymer synthesis, sugars, lipids, oils, fatty acids or vitamins and are preferably amino acids of the aspartate family, especially methionine or lysine, or derivatives of said amino acids, especially cadaverine. Furthermore, the present invention relates to a recombinant microorganism having a reduced isocitrate dehydrogenase activity in comparison to the initial microorganism and the use of such microorganisms in producing fine chemicals such as aspartate family amino acids and their derivatives.
摘要翻译： 本发明涉及利用具有降低的异柠檬酸脱氢酶活性的微生物来生产精细化学品的方法。 所述精细化学品可以是氨基酸，用于聚合物合成的单体，糖，脂质，油，脂肪酸或维生素，并且优选天冬氨酸家族的氨基酸，特别是甲硫氨酸或赖氨酸，或所述氨基酸的衍生物，特别是尸胺。 此外，本发明涉及与初始微生物相比具有降低的异柠檬酸脱氢酶活性的重组微生物，以及这些微生物在生产精细化学品如天冬氨酸家族氨基酸及其衍生物中的用途。
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10.发明授权公开(公告)号：US08252555B2
公开(公告)日：2012-08-28
申请号：US12521141
申请日：2007-12-21
申请人： Oskar Zelder , Wolfgang Grabarse , Corinna Klopprogge , Hartwig Schröder , Stefan Haefner , Anja Knietsch , Andrea Herold
发明人： Oskar Zelder , Wolfgang Grabarse , Corinna Klopprogge , Hartwig Schröder , Stefan Haefner , Anja Knietsch , Andrea Herold
IPC分类号： C12P21/06
CPC分类号： C12N9/0091 , C12P13/12
摘要： The present invention relates to nucleotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof that are modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of such nucleotide sequences.
摘要翻译： 本发明涉及编码酶促活性钴胺素 - 甲硫氨酸合成酶的核苷酸序列及其功能片段，其与相应的野生型酶相比被修饰，使得所述酶显示产品对甲硫氨酸的抑制作用降低。 本发明还涉及由该核苷酸序列编码的多肽和包含该核苷酸序列的宿主细胞。 此外，本发明涉及通过利用这种核苷酸序列在宿主生物体中产生甲硫氨酸的方法。
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