公开(公告)号：US11434526B2

公开(公告)日：2022-09-06

申请号：US16224617

申请日：2018-12-18

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Bing Sun ,  Jesus Rodriguez Manzano ,  Eugenia Khorosheva ,  Matthew S. Curtis

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q1/70

Abstract: The present invention relates to assays, including amplification assays, conducted in the presence of modulators. These assays can be used to detect the presence of particular nucleic acid sequences. In particular, these assays can allow for genotyping or other genetic analysis.

公开(公告)号：US11624062B2

公开(公告)日：2023-04-11

申请号：US16141901

申请日：2018-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Matthew S. Curtis ,  Mary Arrastia ,  David A. Selck ,  Mitchell Guttman

IPC: C12N15/10 ,  B01L3/00 ,  C12Q1/6855 ,  G01N33/58 ,  G01N33/68 ,  C12Q1/6806 ,  C40B30/04 ,  C40B40/08 ,  C40B70/00

Abstract: Methods, systems and related compositions are provided to perform single-cell marking of a nucleic acid and/or protein in a sample based on in-cell or in-organelle barcoding of nucleic acid and/or protein complexes of the cell or organelle; the methods and systems herein described are configured to provide in-cell or in-organelle single-cell marked nucleic acid and/or protein complexes comprising a single-cell, cell-specific, or a single-cell organelle-specific marker.

公开(公告)号：US20180105859A1

公开(公告)日：2018-04-19

申请号：US15715086

申请日：2017-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Nathan G. Schoepp ,  Travis S. Schlappi ,  Matthew S. Curtis

IPC: C12Q1/18 ,  G01N33/58 ,  C12Q1/6851 ,  G01N33/569

CPC classification number: C12Q1/18 ,  C12Q1/10 ,  C12Q1/6851 ,  G01N33/56938 ,  G01N33/582 ,  C12Q2527/101 ,  C12Q2537/165

Abstract: Disclosed herein are methods and devices for using digital isothermal amplification to detect subtle responses to environmental stimuli, such as detecting antibiotic susceptibility using digital quantification of DNA replication and/or chromosome segregation.

公开(公告)号：US11542545B2

公开(公告)日：2023-01-03

申请号：US15524449

申请日：2015-11-05

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  Talis Biomedical Corporation

Inventor： Rustem F. Ismagilov ,  Eugenia Khorosheva ,  Travis S. Schlappi ,  Matthew S. Curtis ,  Nathan G. Schoepp ,  Hedia Maamar ,  Feng Shen ,  Erik B. Jue

IPC: C12Q1/6851 ,  C12Q1/689 ,  C12Q1/6809 ,  C12Q1/18 ,  C12Q1/686 ,  B01L3/00 ,  B01L7/00

Abstract: Disclosed herein are methods and devices for rapid assessment of whether a microorganism present in a sample is susceptible or resistant to a treatment.

公开(公告)号：US11168347B2

公开(公告)日：2021-11-09

申请号：US15715086

申请日：2017-09-25

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Nathan G. Schoepp ,  Travis S. Schlappi ,  Matthew S. Curtis

IPC: C12Q1/18 ,  C12Q1/6851 ,  C12Q1/10

Abstract: Disclosed herein are methods and devices for using digital isothermal amplification to detect subtle responses to environmental stimuli, such as detecting antibiotic susceptibility using digital quantification of DNA replication and/or chromosome segregation.

公开(公告)号：US10196684B2

公开(公告)日：2019-02-05

申请号：US15030202

申请日：2014-10-16

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rustem F. Ismagilov ,  Bing Sun ,  Jesus Rodriguez Manzano ,  Eugenia Khorosheva ,  Matthew S. Curtis

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q1/70

Abstract: The present invention relates to assays, including amplification assays, conducted in the presence of modulators. These assays can be used to detect the presence of particular nucleic acid sequences. In particular, these assays can allow for genotyping or other genetic analysis.