公开(公告)号：US20230211310A1

公开(公告)日：2023-07-06

申请号：US18120694

申请日：2023-03-13

Applicant: CYTONOME/ST, LLC

Inventor： Johnathan Charles Sharpe ,  Donald Francis Perrault, JR.

IPC: B01J19/00 ,  B01L3/00 ,  G01N15/14

CPC classification number: B01J19/0093 ,  B01L3/502715 ,  G01N15/1404 ,  B01J2219/0097 ,  B01J2219/00826 ,  B01J2219/00891 ,  B01J2219/00934 ,  B01L2300/08

Abstract: Systems and methods taught herein enable simultaneous forward and side detection of light originating within a microfluidic channel disposed in a substrate. At least a portion of the microfluidic channel is located in the substrate relative to a first side surface of the substrate to enable simultaneous detection paths with respect to extinction (i.e., 0°) and side detection (i.e., 90°). The location of the microfluidic channel as taught herein enables a maximal half-angle for a ray of light passing from a center of the portion of the microfluidic channel through the first side surface to be in a range from 25 to 90 degrees in some embodiments. By placing at least the portion of the microfluidic channel proximate to the side surface of the substrate, a significantly greater proportion of light emitted or scattered from a particle within the microfluidic channel can be collected and imaged on a detector as compared to conventional particle processing chips.

公开(公告)号：US20210205808A1

公开(公告)日：2021-07-08

申请号：US17211480

申请日：2021-03-24

Applicant: CYTONOME/ST, LLC

Inventor： Donald Francis Perrault, JR. ,  Johnathan Charles Sharpe ,  Erin Koksal

IPC: B01L3/00 ,  G01N35/00 ,  G01N21/00

Abstract: A microfluidic chip assembly having a plurality of microfluidic flow channels is provided. Each channel has a switching region. The microfluidic chip may further include at least one bubble jet actuator configured to generate a pressure pulse in the switching regions of the channels to selectively deflect particles in the flow. The bubble jet actuator may be configured as a blind chamber, as an operative non-through flow chamber and/or as a self-replenishment chamber. The bubble jet actuator may include a trapped air bubble. The bubble jet actuator may include a plurality of heating elements individually controlled for pre-nucleation warmup and/or for triggering vapor bubble nucleation.

公开(公告)号：US20210077973A1

公开(公告)日：2021-03-18

申请号：US17018760

申请日：2020-09-11

Applicant: CYTONOME/ST, LLC

Inventor： Johnathan Charles Sharpe ,  Donald Francis Perrault, JR.

IPC: B01J19/00

Abstract: Systems and methods taught herein enable simultaneous forward and side detection of light originating within a microfluidic channel disposed in a substrate. At least a portion of the microfluidic channel is located in the substrate relative to a first side surface of the substrate to enable simultaneous detection paths with respect to extinction (i.e., 0°) and side detection (i.e., 90°). The location of the microfluidic channel as taught herein enables a maximal half-angle for a ray of light passing from a center of the portion of the microfluidic channel through the first side surface to be in a range from 25 to 90 degrees in some embodiments. By placing at least the portion of the microfluidic channel proximate to the side surface of the substrate, a significantly greater proportion of light emitted or scattered from a particle within the microfluidic channel can be collected and imaged on a detector as compared to conventional particle processing chips.

公开(公告)号：US20210080368A1

公开(公告)日：2021-03-18

申请号：US17019044

申请日：2020-09-11

Applicant: CYTONOME/ST, LLC

Inventor： Johnathan Charles Sharpe ,  Donald Francis Perrault, JR. ,  Peter Kiesel

IPC: G01N15/02

Abstract: Systems and methods taught herein advantageously provide extended dynamic range capabilities to detect low intensity and high intensity emitted or scattered light from particles at high speeds with high sensitivity. Independently controlled first and second optical detector elements that handle light intensities in different dynamic ranges, large overall dynamic range is created. Signals from the detector elements can be combined to create a single combined signal that has excellent sensitivity over a large dynamic range. The detector systems and methods taught herein are particularly advantageous in particle processing where the population of particles can emit or scatter light over a large range of intensity values. Systems and methods taught herein enable a wide dynamic range, optical signals of related to particles of interest within a single detector's dynamic range can be acquired while other optical signals at light intensities outside the single detector's dynamic range can also be accurately captured.

公开(公告)号：US20150276576A1

公开(公告)日：2015-10-01

申请号：US14739812

申请日：2015-06-15

Applicant: CYTONOME/ST, LLC

Inventor： Johnathan Charles Sharpe ,  Emanuel Tito Mendes Machado ,  Blair D. Morad ,  Rudolf Hulspas ,  Donald Francis Perrault, JR.

IPC: G01N15/14 ,  G01N21/64 ,  G01N21/53

CPC classification number: G01N15/1484 ,  G01N15/1012 ,  G01N15/1056 ,  G01N15/1429 ,  G01N15/1459 ,  G01N21/53 ,  G01N21/64 ,  G01N2015/1018 ,  G01N2015/1075 ,  G01N2015/1402 ,  G01N2015/149

Abstract: A microfluidic multiple channel particle analysis system which allows particles from a plurality of particle sources to be independently simultaneously entrained in a corresponding plurality of fluid streams for analysis and sorting into particle subpopulations based upon one or more particle characteristics.

Abstract translation: 微流体多通道粒子分析系统允许来自多个颗粒源的颗粒被独立地同时夹带在相应的多个流体流中，用于基于一个或多个颗粒特征进行分析和分选成颗粒亚群。

公开(公告)号：US20140370536A1

公开(公告)日：2014-12-18

申请号：US14210366

申请日：2014-03-13

Applicant: CYTONOME/ST, LLC

Inventor： Johnathan Charles Sharpe ,  Donald Francis Perrault, JR. ,  Emanuel Tito Mendes Machado ,  Blair D. Morad

IPC: G01N15/14

CPC classification number: G01N15/1434 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1429

Abstract: The present disclosure relates to optical crosstalk reduction in particle processing (e.g., cytometry including flow cytometry using microfluidic based sorters, drop formation based sorters, and/or cell purification) systems and methods in order to improve performance. More particularly, the present disclosure relates to assemblies, systems and methods for minimizing optical crosstalk during the analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting, monitoring and/or enriching) of particles (e.g., cells, microscopic particles, etc.). The exemplary systems and methods for crosstalk reduction in particle processing systems (e.g., cell purification systems) may be particularly useful in the area of cellular medicine or the like. The systems and methods may be modular and used singly or in combination to optimize cell purification based on the crosstalk environment and specific requirements of the operator and/or system.

Abstract translation: 本公开内容涉及粒子处理中的光学串扰降低（例如，包括使用基于微流体的分选器的流式细胞术，基于液滴形成的分选器和/或细胞纯化的细胞计数）系统和方法以提高性能。 更具体地，本公开涉及用于在分析，分选和/或处理（例如，净化，测量，分离，检测，监测和/或富集）颗粒（例如，细胞）期间最小化光学串扰的组件，系统和方法 ，微观粒子等）。 用于粒子处理系统（例如，细胞纯化系统）中的串扰降低的示例性系统和方法可以在细胞医学等领域中特别有用。 系统和方法可以是模块化的，可以单独使用或组合使用，以根据串扰环境和操作者和/或系统的具体要求优化细胞纯化。

公开(公告)号：US20140085898A1

公开(公告)日：2014-03-27

申请号：US14029485

申请日：2013-09-17

Applicant: CYTONOME/ST, LLC

Inventor： Donald Francis Perrault, JR.

IPC: G02B27/12

CPC classification number: G02B27/12 ,  B01L3/502715 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/149 ,  G02B3/10

Abstract: Focal plane shift elements and optical systems with focal plane shifting features for illuminating flow-paths in a fluidic processing system are disclosed. An optical system may include a light source providing an incident first light beam. The optical system may include at least one optical element configured to collect and focus the incident first light beam to produce a second light beam having different portions simultaneously focused at two or more different locations along an optical path, with each location corresponding to a different flow-path of the fluidic processing system. The focal plane shift elements and optical systems with focal plane shifting features may be particularly useful in a microfluidic system.

Abstract translation: 公开了具有用于在流体处理系统中照射流动路径的焦平面偏移特征的焦平面移动元件和光学系统。 光学系统可以包括提供入射的第一光束的光源。 光学系统可以包括至少一个光学元件，其被配置为收集和聚焦入射的第一光束以产生具有不同部分的第二光束，该第二光束同时聚焦在沿着光路的两个或更多个不同位置处，每个位置对应于不同的流 流体处理系统的路线。 具有焦平面偏移特征的焦平面偏移元件和光学系统在微流体系统中可能是特别有用的。

公开(公告)号：US20220187188A1

公开(公告)日：2022-06-16

申请号：US17686974

申请日：2022-03-04

Applicant: CYTONOME/ST, LLC

Inventor： Johnathan Charles Sharpe ,  Donald Francis Perrault, JR.

IPC: G01N15/14 ,  G01N21/64

Abstract: The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles.

公开(公告)号：US20150328637A1

公开(公告)日：2015-11-19

申请号：US14715052

申请日：2015-05-18

Applicant: CYTONOME/ST, LLC

Inventor： Donald Francis Perrault, JR. ,  Johnathan Charles Sharpe ,  Erin Koksal

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0442 ,  B01L2400/0487 ,  B01L2400/0622 ,  G01N2015/1081

Abstract: A microfluidic chip assembly having a plurality of microfluidic flow channels is provided. Each channel has a switching region. The microfluidic chip may further include at least one bubble jet actuator configured to generate a pressure pulse in the switching regions of the channels to selectively deflect particles in the flow. The bubble jet actuator may be configured as a blind chamber, as an operative non-through flow chamber and/or as a self-replenishment chamber. The bubble jet actuator may include a trapped air bubble. The bubble jet actuator may include a plurality of heating elements individually controlled for pre-nucleation warmup and/or for triggering vapor bubble nucleation.

Abstract translation: 提供了具有多个微流体流动通道的微流体芯片组件。 每个通道都有一个切换区域。 微流体芯片还可以包括至少一个气泡喷射致动器，其构造成在通道的切换区域中产生压力脉冲以选择性地偏转流中的颗粒。 气泡喷射致动器可以被配置为盲室，作为可操作的非直流室和/或作为自补充室。 气泡喷射致动器可以包括被捕获的气泡。 气泡喷射致动器可以包括单独控制用于预成核预热和/或用于触发蒸汽气泡成核的多个加热元件。

公开(公告)号：US20140339445A1

公开(公告)日：2014-11-20

申请号：US14208283

申请日：2014-03-13

Applicant: CYTONOME/ST, LLC

Inventor： Johnathan Charles Sharpe ,  Donald Francis Perrault, JR.

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/64 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/149 ,  G02B3/0037 ,  G02B3/0087 ,  G02B5/09

Abstract: The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles.

Abstract translation: 本公开提供了改进的用于颗粒处理的光学系统（例如，细胞计数，包括基于微流体的分选机，滴定分离器和/或细胞纯化）系统和方法。 更具体地，本公开提供了用于颗粒（例如，细胞，微观颗粒等）处理系统和方法的有利的微透镜阵列光学检测组件（例如，用于分析，分选，加工，净化，测量，分离，检测， 监测和/或富集颗粒。