公开(公告)号：US20170372002A1

公开(公告)日：2017-12-28

申请号：US15631832

申请日：2017-06-23

申请人： Canon U.S. Life Sciences, Inc.

发明人： Yang Yang ,  Sophie Paquerault ,  Bradley Scott Denney ,  Lance Charlton ,  Jeanette Paek ,  Attaullah Seikh ,  Vyshnnavi Parthasarathy ,  Ken Pearson ,  Hung Huang ,  Tejinder Uppal

IPC分类号： G06F19/20 ,  B01L3/00 ,  G01N35/00 ,  C12Q1/68 ,  G01N25/04 ,  G06F19/28 ,  G06F19/26

CPC分类号： G16B25/00 ,  B01L3/5027 ,  B01L2300/02 ,  B01L2300/023 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/18 ,  C12Q1/68 ,  C12Q1/6851 ,  G01N25/04 ,  G01N25/4846 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2035/00148 ,  G01N2035/00366 ,  G01N2035/00881 ,  G01N2035/0091 ,  G16B25/20 ,  G16B45/00 ,  G16B50/00 ,  C12Q2527/107 ,  C12Q2537/165

摘要： The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).

公开(公告)号：US11232851B2

公开(公告)日：2022-01-25

申请号：US15631832

申请日：2017-06-23

申请人： Canon U.S. Life Sciences, Inc.

发明人： Yang Yang ,  Sophie Paquerault ,  Bradley Scott Denney ,  Lance Charlton ,  Jeanette Paek ,  Attaullah Seikh ,  Vyshnnavi Parthasarathy ,  Ken Pearson ,  Hung Huang ,  Tejinder Uppal

IPC分类号： G16B25/00 ,  G01N25/04 ,  C12Q1/68 ,  G01N35/00 ,  G16B45/00 ,  G16B50/00 ,  G01N25/48 ,  G16B25/20 ,  G16B5/30 ,  B01L3/00 ,  C12Q1/6851

摘要： The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).