公开(公告)号：US11232851B2

公开(公告)日：2022-01-25

申请号：US15631832

申请日：2017-06-23

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Yang Yang ,  Sophie Paquerault ,  Bradley Scott Denney ,  Lance Charlton ,  Jeanette Paek ,  Attaullah Seikh ,  Vyshnnavi Parthasarathy ,  Ken Pearson ,  Hung Huang ,  Tejinder Uppal

IPC: G16B25/00 ,  G01N25/04 ,  C12Q1/68 ,  G01N35/00 ,  G16B45/00 ,  G16B50/00 ,  G01N25/48 ,  G16B25/20 ,  G16B5/30 ,  B01L3/00 ,  C12Q1/6851

Abstract: The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).

公开(公告)号：US20180011969A1

公开(公告)日：2018-01-11

申请号：US15631794

申请日：2017-06-23

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Yang Yang ,  Bradley Scott Denney ,  Attaullah Seikh

IPC: G06F19/24 ,  G01N21/64 ,  G01N25/04 ,  G06F17/18

CPC classification number: G16B40/00 ,  C12Q1/6844 ,  G01N21/6428 ,  G01N25/04 ,  G01N2021/6439 ,  G06F17/18 ,  G16B20/20 ,  G16B40/30 ,  C12Q2527/107 ,  C12Q2531/113 ,  C12Q2537/165 ,  C12Q2561/113 ,  C12Q2563/107

Abstract: The present invention relates to methods and systems for the analysis of nucleic acids present in biological samples, and more specifically, relates to clustering melt curves derived from high resolution thermal melt analysis performed on a sample of nucleic acids, the resulting clusters being usable, in one embodiment, for analyzing the sequences of nucleic acids and to classify their genotypes that are useful for determining the identity of the genotype of a nucleic acid that is present in a biological sample.

公开(公告)号：US11332781B2

公开(公告)日：2022-05-17

申请号：US15724061

申请日：2017-10-03

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Yang Yang ,  Sophie Paquerault ,  Bradley Scott Denney ,  Lingxia Jiang

IPC: C12Q1/6858 ,  B01L7/00 ,  B01L3/00 ,  G01N25/04 ,  G16B25/20 ,  G16B50/30 ,  G16B50/00 ,  G16B45/00 ,  C12Q1/6851

Abstract: The present invention relates to a method and system for determining Copy Number Variations (CNVs) in a genomic test sample including target amplicons and a reference amplicons. Specifically, nucleic acid melting curves are generated for the test sample. A mathematical model is fitted to each of the nucleic acid melting curves to separate target and reference melting reactions within the measured nucleic acid melting curve. The fitting parameters of the mathematical model are calculated. A CNV of the test sample is determined based on the fitting parameters of the mathematical model corresponding to the target and reference melting reactions.

公开(公告)号：US11056214B2

公开(公告)日：2021-07-06

申请号：US15631794

申请日：2017-06-23

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Yang Yang ,  Bradley Scott Denney ,  Attaullah Seikh

IPC: G16B40/00 ,  G06F17/18 ,  G01N21/64 ,  G01N25/04 ,  C12Q1/6844 ,  G16B20/20 ,  G16B40/30

Abstract: The present invention relates to methods and systems for the analysis of nucleic acids present in biological samples, and more specifically, relates to clustering melt curves derived from high resolution thermal melt analysis performed on a sample of nucleic acids, the resulting clusters being usable, in one embodiment, for analyzing the sequences of nucleic acids and to classify their genotypes that are useful for determining the identity of the genotype of a nucleic acid that is present in a biological sample.

公开(公告)号：US20180073070A1

公开(公告)日：2018-03-15

申请号：US15724061

申请日：2017-10-03

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Yang Yang ,  Sophie Paquerault ,  Bradley Scott Denney ,  Lingxia Jiang

IPC: C12Q1/68 ,  B01L7/00 ,  B01L3/00 ,  G01N25/04 ,  G06F19/26

CPC classification number: C12Q1/6858 ,  B01L3/5027 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2300/023 ,  B01L2300/0627 ,  C12Q1/6851 ,  G01N25/04 ,  G16B25/20 ,  G16B45/00 ,  G16B50/30 ,  C12Q2527/107 ,  C12Q2537/165

Abstract: The present invention relates to a method and system for determining Copy Number Variations (CNVs) in a genomic test sample including target amplicons and a reference amplicons. Specifically, nucleic acid melting curves are generated for the test sample. A mathematical model is fitted to each of the nucleic acid melting curves to separate target and reference melting reactions within the measured nucleic acid melting curve. The fitting parameters of the mathematical model are calculated. A CNV of the test sample is determined based on the fitting parameters of the mathematical model corresponding to the target and reference melting reactions.

公开(公告)号：US20170372002A1

公开(公告)日：2017-12-28

申请号：US15631832

申请日：2017-06-23

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Yang Yang ,  Sophie Paquerault ,  Bradley Scott Denney ,  Lance Charlton ,  Jeanette Paek ,  Attaullah Seikh ,  Vyshnnavi Parthasarathy ,  Ken Pearson ,  Hung Huang ,  Tejinder Uppal

IPC: G06F19/20 ,  B01L3/00 ,  G01N35/00 ,  C12Q1/68 ,  G01N25/04 ,  G06F19/28 ,  G06F19/26

CPC classification number: G16B25/00 ,  B01L3/5027 ,  B01L2300/02 ,  B01L2300/023 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/18 ,  C12Q1/68 ,  C12Q1/6851 ,  G01N25/04 ,  G01N25/4846 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2035/00148 ,  G01N2035/00366 ,  G01N2035/00881 ,  G01N2035/0091 ,  G16B25/20 ,  G16B45/00 ,  G16B50/00 ,  C12Q2527/107 ,  C12Q2537/165

Abstract: The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).