Isobutanol production using yeasts with modified transporter expression
    1.
    发明授权
    Isobutanol production using yeasts with modified transporter expression 有权
    使用具有改良转运蛋白表达的酵母的异丁醇生产

    公开(公告)号:US09249420B2

    公开(公告)日:2016-02-02

    申请号:US13701247

    申请日:2011-05-31

    摘要: The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise one or more modifications resulting in the reduction in the expression and/or activity of an endogenous transporter protein. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

    摘要翻译: 本发明涉及包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面,重组微生物可包含导致内源转运蛋白的表达和/或活性降低的一种或多种修饰。 在本文所述的各种实施方案中,重组微生物可以是酵母进化枝,Crabtree阴性酵母微生物,Crabtree阳性酵母微生物,WGD(全基因组复制)酵母微生物,WGD前(全基因组复制)酵母微生物 ,和非发酵酵母微生物。

    Isobutanol Production Using Yeasts With Modified Transporter Expression
    2.
    发明申请
    Isobutanol Production Using Yeasts With Modified Transporter Expression 有权
    使用改良转运蛋白表达酵母的异丁醇生产

    公开(公告)号:US20130302868A1

    公开(公告)日:2013-11-14

    申请号:US13701247

    申请日:2011-05-31

    IPC分类号: C12N15/81 C12P7/16

    摘要: The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise one or more modifications resulting in the reduction in the expression and/or activity of an endogenous transporter protein. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces Glade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

    摘要翻译: 本发明涉及包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面,重组微生物可包含导致内源转运蛋白的表达和/或活性降低的一种或多种修饰。 在本文所述的各种实施方案中,重组微生物可以是葡萄糖酵母的微生物,Crabtree阴性酵母微生物,Crabtree阳性酵母微生物,WGD(全基因组复制)酵母微生物,前WGD(全基因组复制)酵母微生物 ,和非发酵酵母微生物。

    Engineered Microorganisms for the Production of One or More Target Compounds
    5.
    发明申请
    Engineered Microorganisms for the Production of One or More Target Compounds 审中-公开
    用于生产一种或多种靶化合物的工程微生物

    公开(公告)号:US20120045809A1

    公开(公告)日:2012-02-23

    申请号:US13141648

    申请日:2009-12-23

    IPC分类号: C12P7/16 C12N1/19

    摘要: The present invention provides recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms comprise isobutanol producing metabolic pathway with one or more isobutanol pathway enzymes localized in the mitochondria. In various embodiments described herein, the recombinant microorganisms may be Crabtree-negative yeast microorganisms, microorganisms of the Saccharomyces clade, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

    摘要翻译: 本发明提供包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面,重组微生物包含产生异丁醇的代谢途径与一种或多种位于线粒体中的异丁醇途径酶。 在本文所述的各种实施方案中,重组微生物可以是Crabtree阴性酵母微生物,酵母进化枝的微生物,Crabtree阳性酵母微生物,WGD(全基因组复制)酵母微生物,WGD前(全基因组复制)酵母微生物 ,和非发酵酵母微生物。

    YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD
    6.
    发明申请
    YEAST ORGANISM PRODUCING ISOBUTANOL AT A HIGH YIELD 审中-公开
    YEAST ORGANISM在高产量下生产ISOBUTANOL

    公开(公告)号:US20110318799A1

    公开(公告)日:2011-12-29

    申请号:US13229438

    申请日:2011-09-09

    IPC分类号: C12P7/16

    摘要: There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

    摘要翻译: 公开了异丁醇的制造方法。 在一个实施方案中,该方法包括提供转化了含有至少一种外源基因的产生异丁醇的途径的微生物。 选择微生物以产生至少10%的理论值从碳源产生异丁醇。 该方法包括在含有提供碳源的原料的培养基中培养微生物,直到产生异丁醇。 该方法包括回收异丁醇。 在一个实施方案中,微生物是具有Crabtree-阴性表型的酵母。 在另一个实施方案中,微生物是具有Crabtree阳性表型的酵母微生物。 公开了用于生产异丁醇的微生物。 在一个实施方案中,微生物包括含有至少一种外源基因的异丁醇生产途径,并且选择其以至少10%理论值的产率从碳源产生可回收量的异丁醇。

    Yeast organism producing isobutanol at a high yield
    10.
    发明授权
    Yeast organism producing isobutanol at a high yield 无效
    以高产率生产异丁醇的酵母生物

    公开(公告)号:US08017375B2

    公开(公告)日:2011-09-13

    申请号:US12343375

    申请日:2008-12-23

    摘要: There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

    摘要翻译: 公开了异丁醇的制造方法。 在一个实施方案中,该方法包括提供转化了含有至少一种外源基因的产生异丁醇的途径的微生物。 选择微生物以产生至少10%的理论值从碳源产生异丁醇。 该方法包括在含有提供碳源的原料的培养基中培养微生物,直到产生异丁醇。 该方法包括回收异丁醇。 在一个实施方案中,微生物是具有Crabtree-阴性表型的酵母。 在另一个实施方案中,微生物是具有Crabtree阳性表型的酵母微生物。 公开了用于生产异丁醇的微生物。 在一个实施方案中,微生物包括含有至少一种外源基因的异丁醇生产途径,并且选择其以至少10%理论值的产率从碳源产生可回收量的异丁醇。