公开(公告)号：US09249420B2

公开(公告)日：2016-02-02

申请号：US13701247

申请日：2011-05-31

申请人： Catherine Asleson Dundon ,  Christopher Smith ,  Piruz Nahreini ,  Johan Thevelein ,  Sofie Saerens

发明人： Catherine Asleson Dundon ,  Christopher Smith ,  Piruz Nahreini ,  Johan Thevelein ,  Sofie Saerens

IPC分类号： C12P7/16 ,  C12N15/81 ,  C12N1/00 ,  C07K14/395

CPC分类号： C12P7/16 ,  C07K14/395 ,  C12N1/00 ,  C12N1/16 ,  C12N15/81 ,  Y02E50/10

摘要： The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise one or more modifications resulting in the reduction in the expression and/or activity of an endogenous transporter protein. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

摘要翻译： 本发明涉及包含产生异丁醇的代谢途径的重组微生物和使用所述重组微生物产生异丁醇的方法。 在本发明的各个方面，重组微生物可包含导致内源转运蛋白的表达和/或活性降低的一种或多种修饰。 在本文所述的各种实施方案中，重组微生物可以是酵母进化枝，Crabtree阴性酵母微生物，Crabtree阳性酵母微生物，WGD（全基因组复制）酵母微生物，WGD前（全基因组复制）酵母微生物 ，和非发酵酵母微生物。

公开(公告)号：US20050283852A1

公开(公告)日：2005-12-22

申请号：US11065184

申请日：2005-02-24

申请人： Gabriel De La Fuente ,  Johan Thevelein ,  Patrick Duck ,  Jose Mascorro-Gallardo ,  Christophe Vaeck

发明人： Gabriel De La Fuente ,  Johan Thevelein ,  Patrick Duck ,  Jose Mascorro-Gallardo ,  Christophe Vaeck

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N9/10 ,  C12N9/16 ,  C12N15/09 ,  C12N15/29 ,  C12N15/54 ,  C12N15/55 ,  C12N15/62 ,  C12N15/82 ,  C12Q1/25 ,  A01H1/00

CPC分类号： C12N9/2402 ,  C07K2319/00 ,  C12N9/1051 ,  C12N9/16 ,  C12N15/8245 ,  C12Q1/54

摘要： A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5′ terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5′ terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

公开(公告)号：US20100024066A1

公开(公告)日：2010-01-28

申请号：US12087710

申请日：2007-01-29

申请人： Barbara Leyman ,  Matthew Ramon ,  Filip Rolland ,  Johan Thevelein ,  Patrick van Dijck ,  Lies Vandesteene

发明人： Barbara Leyman ,  Matthew Ramon ,  Filip Rolland ,  Johan Thevelein ,  Patrick van Dijck ,  Lies Vandesteene

IPC分类号： A01H1/06

CPC分类号： C12N15/8261 ,  C12N9/1051 ,  Y02A40/146

摘要： The present invention relates to the use of trehalose-6-phosphate synthase to modulate plant growth. More specifically, it relates to the use of a class II trehalose-6-phosphate synthase, comprising both a synthase and a phosphatase-like part to modulate plant growth. Preferably, the activity of trehalose-6-phosphate synthase is down-regulated to obtain an increased plant biomass yield.

摘要翻译： 本发明涉及海藻糖-6-磷酸合成酶调节植物生长的用途。 更具体地说，本发明涉及使用包含合酶和磷酸酶样部分的II类海藻糖-6-磷酸合成酶来调节植物生长。 优选地，海藻糖-6-磷酸合酶的活性被下调以获得增加的植物生物量产量。

公开(公告)号：US08357835B2

公开(公告)日：2013-01-22

申请号：US12087710

申请日：2007-01-29

申请人： Barbara Leyman ,  Matthew Ramon ,  Filip Rolland ,  Johan Thevelein ,  Patrick van Dijck ,  Lies Vandesteene

发明人： Barbara Leyman ,  Matthew Ramon ,  Filip Rolland ,  Johan Thevelein ,  Patrick van Dijck ,  Lies Vandesteene

IPC分类号： C12N15/82

CPC分类号： C12N15/8261 ,  C12N9/1051 ,  Y02A40/146

摘要： The present invention relates to the use of trehalose-6-phosphate synthase to modulate plant growth. More specifically, it relates to the use of a class II trehalose-6-phosphate synthase, comprising both a synthase and a phosphatase-like part to modulate plant growth. Preferably, the activity of trehalose-6-phosphate synthase is down-regulated to obtain an increased plant biomass yield.

摘要翻译： 本发明涉及海藻糖-6-磷酸合成酶调节植物生长的用途。 更具体地说，本发明涉及使用包含合酶和磷酸酶样部分的II类海藻糖-6-磷酸合成酶来调节植物生长。 优选地，海藻糖-6-磷酸合酶的活性被下调以获得增加的植物生物量产量。

公开(公告)号：US20100024067A1

公开(公告)日：2010-01-28

申请号：US12518909

申请日：2007-12-13

申请人： Ana Isabel Sanz Molinero ,  Lies Vandesteene ,  Matthew Ramon ,  Filip Rolland ,  Patrick Van Dijck ,  Johan Thevelein

发明人： Ana Isabel Sanz Molinero ,  Lies Vandesteene ,  Matthew Ramon ,  Filip Rolland ,  Patrick Van Dijck ,  Johan Thevelein

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/00 ,  A01H5/00 ,  C12N5/10 ,  A01H5/10

CPC分类号： C12N15/8261 ,  C12N9/16 ,  Y02A40/146

摘要： The present invention concerns a method for enhancing yield-related traits in plants, particularly increasing seed yield, by modulating expression in a plant of a nucleic acid encoding a Class III Trehalose Phosphate Phosphatase (TPP) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a Class III TPP polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention further concerns novel Class III TPP nucleic acid and polypeptide sequences. The invention also provides nucleic acid and polypeptide sequences, and constructs comprising the same, which are useful in the methods of the invention.

摘要翻译： 本发明涉及通过调节植物中编码III类海藻糖磷酸酯磷酸酶（TPP）多肽的核酸的表达来提高植物中产量相关性状的方法，特别是增加种子产量。 本发明还涉及已经调节表达编码III类TPP多肽的核酸的植物，该植物相对于对照植物具有增强的产量相关性状。 本发明还涉及新型III类TPP核酸和多肽序列。 本发明还提供了可用于本发明方法的核酸和多肽序列及包含其的构建体。