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公开(公告)号：US09175338B2
公开(公告)日：2015-11-03
申请号：US12945767
申请日：2010-11-12
申请人： Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
发明人： Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要： Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译： 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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公开(公告)号：US20120003699A1
公开(公告)日：2012-01-05
申请号：US13171766
申请日：2011-06-29
申请人： Cheryl Heiner , Stephen Turner , Kevin Travers
发明人： Cheryl Heiner , Stephen Turner , Kevin Travers
CPC分类号： C12Q1/6806 , C12Q1/6869 , C12Q2563/179 , C12Q2563/155 , C12Q2525/307
摘要： Methods of separating tagged nucleic acid fragments from populations of fragments are provided. The fragments are produced from a population of tagged nucleic acids. In some cases, the population of tagged nucleic acids comprises a population of clonal nucleic acids.
摘要翻译： 提供了从片段群体分离标记的核酸片段的方法。 片段由标记核酸群体产生。 在一些情况下，标记核酸的群体包括克隆核酸群体。
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公开(公告)号：US20090105094A1
公开(公告)日：2009-04-23
申请号：US12286119
申请日：2008-09-26
申请人： Cheryl Heiner , Stephen Turner , Kevin Travers
发明人： Cheryl Heiner , Stephen Turner , Kevin Travers
IPC分类号： C40B50/06
CPC分类号： C12Q1/6806 , C12Q1/6869 , C12Q2563/179 , C12Q2563/155 , C12Q2525/307
摘要： Methods of preparing nucleic acid templates and providing the nucleic acid templates to low copy number reaction volumes are provided. Related compositions of nucleic acid templates are also provided.
摘要翻译： 提供了制备核酸模板并将核酸模板提供给低拷贝数反应体积的方法。 还提供了核酸模板的相关组合物。
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公开(公告)号：US08153375B2
公开(公告)日：2012-04-10
申请号：US12413258
申请日：2009-03-27
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US08003330B2
公开(公告)日：2011-08-23
申请号：US12286119
申请日：2008-09-26
申请人： Cheryl Heiner , Stephen Turner , Kevin Travers
发明人： Cheryl Heiner , Stephen Turner , Kevin Travers
CPC分类号： C12Q1/6806 , C12Q1/6869 , C12Q2563/179 , C12Q2563/155 , C12Q2525/307
摘要： Provided are methods of producing low copy number circularized nucleic acid variants that can be distributed to reaction volumes. The methods include providing a template nucleic acid; producing a population of clonal nucleic acids from the template nucleic acid; generating a set of partially overlapping nucleic acid fragments from the population of clonal nucleic acids; circularizing the partially overlapping nucleic acid fragments to produce circularized nucleic acid variants; and aliquotting the circularized nucleic acid variants into reaction volumes. Related compositions of nucleic acid templates are also provided.
摘要翻译： 提供了可以分配到反应体积的低拷贝数环状核酸变体的生产方法。 所述方法包括提供模板核酸; 从模板核酸产生克隆核酸群; 从克隆核酸群体产生一组部分重叠的核酸片段; 使部分重叠的核酸片段环化以产生环化的核酸变体; 并将环化的核酸变体等分成反应体积。 还提供了核酸模板的相关组合物。
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公开(公告)号：US08455193B2
公开(公告)日：2013-06-04
申请号：US13403789
申请日：2012-02-23
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US08309330B2
公开(公告)日：2012-11-13
申请号：US13019220
申请日：2011-02-01
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Methods, substrates, and systems for diagnostic sequencing are provided. Small circles of nucleic acids from about 10 bases to about 200 bases can be sequenced, for example using template dependent sequencing by synthesis. The use of small circles of nucleic acids allows for repeated sequencing of the same portions of the nucleic acid, providing for higher accuracy sequence determinations.
摘要翻译： 提供了用于诊断测序的方法，底物和系统。 可以对约10个碱基至约200个碱基的小核酸核酸进行测序，例如使用通过合成进行模板依赖性测序。 核酸的小圈的使用允许对核酸的相同部分进行重复测序，提供更高精度的序列测定。
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公开(公告)号：US20120289408A1
公开(公告)日：2012-11-15
申请号：US13403789
申请日：2012-02-23
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US20110281768A1
公开(公告)日：2011-11-17
申请号：US13019220
申请日：2011-02-01
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Methods, substrates, and systems for diagnostic sequencing are provided. Small circles of nucleic acids from about 10 bases to about 200 bases can be sequenced, for example using template dependent sequencing by synthesis. The use of small circles of nucleic acids allows for repeated sequencing of the same portions of the nucleic acid, providing for higher accuracy sequence determinations.
摘要翻译： 提供了用于诊断测序的方法，底物和系统。 可以对约10个碱基至约200个碱基的小核酸核酸进行测序，例如使用通过合成进行模板依赖性测序。 核酸的小圈的使用允许对核酸的相同部分进行重复测序，提供更高精度的序列测定。
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公开(公告)号：US20110183320A1
公开(公告)日：2011-07-28
申请号：US12945767
申请日：2010-11-12
申请人： Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
发明人： Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
摘要： Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
摘要翻译： 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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