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公开(公告)号：US20110281768A1
公开(公告)日：2011-11-17
申请号：US13019220
申请日：2011-02-01
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Methods, substrates, and systems for diagnostic sequencing are provided. Small circles of nucleic acids from about 10 bases to about 200 bases can be sequenced, for example using template dependent sequencing by synthesis. The use of small circles of nucleic acids allows for repeated sequencing of the same portions of the nucleic acid, providing for higher accuracy sequence determinations.
摘要翻译： 提供了用于诊断测序的方法，底物和系统。 可以对约10个碱基至约200个碱基的小核酸核酸进行测序，例如使用通过合成进行模板依赖性测序。 核酸的小圈的使用允许对核酸的相同部分进行重复测序，提供更高精度的序列测定。
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公开(公告)号：US20090298075A1
公开(公告)日：2009-12-03
申请号：US12413258
申请日：2009-03-27
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
IPC分类号： C12Q1/68
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US20120289408A1
公开(公告)日：2012-11-15
申请号：US13403789
申请日：2012-02-23
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US08153375B2
公开(公告)日：2012-04-10
申请号：US12413258
申请日：2009-03-27
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US08455193B2
公开(公告)日：2013-06-04
申请号：US13403789
申请日：2012-02-23
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
摘要翻译： 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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公开(公告)号：US08309330B2
公开(公告)日：2012-11-13
申请号：US13019220
申请日：2011-02-01
申请人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
发明人： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC分类号： G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
摘要： Methods, substrates, and systems for diagnostic sequencing are provided. Small circles of nucleic acids from about 10 bases to about 200 bases can be sequenced, for example using template dependent sequencing by synthesis. The use of small circles of nucleic acids allows for repeated sequencing of the same portions of the nucleic acid, providing for higher accuracy sequence determinations.
摘要翻译： 提供了用于诊断测序的方法，底物和系统。 可以对约10个碱基至约200个碱基的小核酸核酸进行测序，例如使用通过合成进行模板依赖性测序。 核酸的小圈的使用允许对核酸的相同部分进行重复测序，提供更高精度的序列测定。
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7.发明授权Reaction mixtures for prevention and alleviation of steric hindrance during single molecule synthesis 有权用于单分子合成期间预防和减轻空间位阻的反应混合物公开(公告)号：US08835135B2
公开(公告)日：2014-09-16
申请号：US13468365
申请日：2012-05-10
申请人： Congcong Ma , Paul Wyatt
发明人： Congcong Ma , Paul Wyatt
摘要： The present invention provides compositions and methods for reducing steric hindrance in the product of nucleic acid polymerase reaction. Methods and compositions of the invention encompass application of exonucleases, endonucleases, and uracil-DNA glycosylases to a nucleic acid polymerase reaction such that newly formed nucleic acid strands are modified (e.g., cleaved) while the polymerase reaction continues to proceed.
摘要翻译： 本发明提供了降低核酸聚合酶反应产物中的空间位阻的组合物和方法。 本发明的方法和组合物包括将核酸外切酶，内切核酸酶和尿嘧啶-DNA糖基酶应用于核酸聚合酶反应，使得新聚合酶反应继续进行而修饰（例如，切割）。
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8.发明授权公开(公告)号：US09194772B2
公开(公告)日：2015-11-24
申请号：US12557488
申请日：2009-09-10
申请人： Linda Lee , Sam Woo , Congcong Ma , Richard Reel , Mark Oldham , David Cox , Benjamin Schroeder , Jon Sorenson , Willy Wiyatno
发明人： Linda Lee , Sam Woo , Congcong Ma , Richard Reel , Mark Oldham , David Cox , Benjamin Schroeder , Jon Sorenson , Willy Wiyatno
IPC分类号： C12Q1/68 , C12M1/34 , G01N1/14 , B01L3/00 , F15C5/00 , F16K99/00 , G01N35/08 , B01L3/02 , B01L7/00
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
摘要： Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.
摘要翻译： 本发明的各种实施方案涉及生物化学或分子生物学程序中用于样品制备或分析的系统或方法。 样品制备可以涉及在离散部分或段或块中的小体积处理，在本文中称为离散体积。 分子生物学程序可以是核酸分析。 核酸分析可以是一个综合的DNA扩增/ DNA测序程序。
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9.发明申请PREVENTION AND ALLEVIATION OF STERIC HINDRANCE DURING SINGLE MOLECULE SYNTHESIS 有权单分子合成期间的绝育情况的预防与评价公开(公告)号：US20100035269A1
公开(公告)日：2010-02-11
申请号：US12535385
申请日：2009-08-04
申请人： Congcong Ma , Paul Wyatt
发明人： Congcong Ma , Paul Wyatt
摘要： The present invention provides compositions and methods for reducing steric hindrance in the product of nucleic acid polymerase reaction. Methods and compositions of the invention encompass application of exonucleases, endonucleases, and uracil-DNA glycosylases to a nucleic acid polymerase reaction such that newly formed nucleic acid strands are modified (e.g., cleaved) while the polymerase reaction continues to proceed.
摘要翻译： 本发明提供了降低核酸聚合酶反应产物中的空间位阻的组合物和方法。 本发明的方法和组合物包括将核酸外切酶，内切核酸酶和尿嘧啶-DNA糖基酶应用于核酸聚合酶反应，使得新聚合酶反应继续进行而修饰（例如，切割）。
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10.发明申请公开(公告)号：US20070141593A1
公开(公告)日：2007-06-21
申请号：US11507735
申请日：2006-08-22
申请人： Linda Lee , Sam Woo , Congcong Ma , Richard Reel , Mark Oldham , David Cox , Benjamin Schroeder , Jon Sorenson , Willy Wiyatno
发明人： Linda Lee , Sam Woo , Congcong Ma , Richard Reel , Mark Oldham , David Cox , Benjamin Schroeder , Jon Sorenson , Willy Wiyatno
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , C12Q2535/101 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575
摘要： Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.
摘要翻译： 本发明的各种实施方案涉及生物化学或分子生物学程序中用于样品制备或分析的系统或方法。 样品制备可以涉及在离散部分或段或块中的小体积处理，在本文中称为离散体积。 分子生物学程序可以是核酸分析。 核酸分析可以是一个综合的DNA扩增/ DNA测序程序。
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