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公开(公告)号：US5312748A
公开(公告)日：1994-05-17
申请号：US223788
申请日：1988-08-30
IPC分类号： C12N9/54 , C11D3/38 , C11D3/386 , C11D7/42 , C12N1/20 , C12N9/50 , C12N9/52 , C12N9/58 , C12N15/00 , C12R1/01 , C12R1/10 , C12R1/365 , C12R1/645 , C12R1/77 , C12R1/79 , C12P21/04 , C12N9/98
摘要： Novel alkaline serine proteases produced by Nocardiopsis sp. strains, for example by aerobic cultivation of strain 10R (NRRL-18262) and Nocardiopsis dassonvillei strain M58-1 (NRRL 18133), the proteases being characterized by a pH optimum of about 8, and by retention of at least 60% of maximum activity at pH 7-11 measured with casein as substrate.The proteases of the invention may be distinguished from the known protease of the Nocardiopsis strain ZIMET 43647 (of East German 2004328) and known Bacillus and fungal alkaline proteases. The Alkaline proteases of strains 10R and M58-1 may also be distinguished from each other.The protease preparations of this invention are useful detergent additives for cold water laundering.
摘要翻译： PCT No.PCT / DK87 / 00144 Sec。 371日期1988年8月30日第 102（e）1988年8月30日PCT PCT公开1987年11月25日PCT公布。 出版物WO88 / 03947 日期：1988年6月2日。由诺卡氏菌（Nocardiopsis sp。）生产的新型碱性丝氨酸蛋白酶 菌株，例如通过需氧培养菌株10R（NRRL-18262）和诺卡氏菌Dassonvillei菌株M58-1（NRRL 18133），蛋白酶的特征在于pH最佳约为8，并通过保留至少60％的最大活性 用酪蛋白作为底物测定pH7-11。 本发明的蛋白酶可以与诺卡氏菌属菌株ZIMET 43647（东德2004328）和已知的芽孢杆菌属和真菌碱性蛋白酶的已知蛋白酶区分开来。 菌株10R和M58-1的碱性蛋白酶也可以彼此区分。 本发明的蛋白酶制剂是用于冷水洗涤的有用的洗涤剂添加剂。
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公开(公告)号：US5374545A
公开(公告)日：1994-12-20
申请号：US86388
申请日：1993-07-01
申请人： Chi-Li Liu , Janet M. Overholt
发明人： Chi-Li Liu , Janet M. Overholt
IPC分类号： C12N9/36 , C12N1/06 , C12N1/20 , C12N9/14 , C12R1/07 , C12R1/15 , C12R1/265 , C12R1/385 , C12R1/43 , C12N9/00 , C12N1/00
CPC分类号： C12R1/07 , C12N9/14 , Y10S435/832
摘要： A bacteriolytic enzyme complex is obtained from a bacterial culture of Bacillus pabuli strains, e.g. , isolates 350-2 (NRRL B-18446) and 391-1 (NRRL B-18447). This bacteriolytic enzyme complex is useful as an antibacterial agent against both Gram-positive and Gram-negative bacteria. The enzyme complex may be produced by cultivating the B. pabuli microorganisms in an aqueous medium containing cornsteep liquor, after which the lyric enzyme complex can be recovered from the fermentation broth.
摘要翻译： 溶菌酶复合物从芽孢杆菌菌株的细菌培养物获得，例如， ，分离出350-2（NRRL B-18446）和391-1（NRRL B-18447）。 这种溶菌酶复合物可用作抗革兰氏阳性和革兰氏阴性细菌的抗菌剂。 酶复合物可以通过在含有玉米浆液的水性培养基中培养B.pabuli微生物来制备，然后从发酵液中回收枸橼酸酶复合物。
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3.发明授权Enzyme complex hydrolyzing bacterial cell walls derived from nocardiopsis dassonvillei 失效酶复合物水解细菌细胞壁源自诺卡氏菌dassonvillei
公开(公告)号：US5354681A
公开(公告)日：1994-10-11
申请号：US196619
申请日：1988-05-17
申请人： Chi-Li Liu , Janet M. Overholt
发明人： Chi-Li Liu , Janet M. Overholt
CPC分类号： C12Y302/01129 , C11D3/38636 , C12N9/2402 , C12N9/2442 , C12N9/2462 , C12Y302/01006 , C12Y302/01014 , Y10S435/822
摘要： A bacteriolytic enzyme complex obtained from a bacterial culture of Nocardiopsis dassonvillei strains, e.g., isolates G102-3 (NRRL 18349), G119-6 (NRRL 18350), and D38-3 (NRRL 18364).The enzyme complex may be produced by cultivating the microorganism in an aqueous medium containing maltodextrin and soybean meal, after which the lytic enzyme complex may be recovered from the fermentation broth.This bacteriolytic enzyme complex is useful as a detergent additive.
摘要翻译： 从Nocardiopsis dassonvillei菌株的细菌培养物获得的溶菌酶复合物，例如分离株G102-3（NRRL 18349），G119-6（NRRL 18350）和D38-3（NRRL 18364）。 可以通过在含有麦芽糖糊精和大豆粉的水性介质中培养微生物来生产酶复合物，之后从发酵液中回收裂解酶复合物。 该溶菌酶复合物可用作洗涤剂添加剂。
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4.发明授权Bacillus thuringiensis mutants which produce higher yields of crystal delta-endotoxin than their corresponding parental strains 失效苏云金芽孢杆菌突变体比其相应的亲本菌株产生更高的晶体δ-内毒素产量公开(公告)号：US06210952B1
公开(公告)日：2001-04-03
申请号：US08902412
申请日：1997-07-29
IPC分类号： C12N120
CPC分类号： C12R1/075 , C07K14/325
摘要： The invention relates to a mutant of Bacillus thuringiensis which produces a larger amount of crystal delta-endotoxin with a greater pesticidal activity as compared to the corresponding parental strain. The mutant may also have a larger crystal size as compared to the corresponding parental strain. The crystal delta-endotoxin produced by the mutant Bacillus thuringiensis will have an activity directed towards the same pest(s) as its parental Bacillus thuringiensis crystal delta-endotoxin. The invention further relates to a method for producing such a mutant, compositions comprising such a mutant as well as methods for controlling a pest(s) using these compositions.
摘要翻译： 本发明涉及与相应的亲本菌株相比产生更大量的具有更大杀虫活性的结晶δ-内毒素的苏云金芽孢杆菌的突变体。 与相应的亲本菌株相比，突变体也可以具有更大的晶体尺寸。 由突变型苏云金芽孢杆菌产生的晶体δ-内毒素将具有针对与其亲本苏云金芽孢杆菌晶体δ-内毒素相同的害虫的活性。 本发明还涉及制备这种突变体的方法，该组合物包含这种突变体以及使用这些组合物来控制害虫的方法。
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公开(公告)号：US5976564A
公开(公告)日：1999-11-02
申请号：US946897
申请日：1997-10-08
申请人： Chi-Li Liu , Anita M. MacMullan , Patricia Ann Lufburrow , Robert L. Starnes , Denise C. Manker
发明人： Chi-Li Liu , Anita M. MacMullan , Patricia Ann Lufburrow , Robert L. Starnes , Denise C. Manker
摘要： The invention is related to a novel strain(s) of Bacillus thuringiensis in which essentially all of the pesticidal activity of said strain is in the supernatant of a fermentation of said strain. The strain produces a substance which has activity against an insect pest(s) of the order Coleoptera and which enhances the pesticidal activity of a Bacillus related pesticide. The invention further relates to pesticidal compositions comprising the substance and a pesticidal carrier, or the substance and a Bacillus related pesticide, a chemical pesticide and/or a virus with pesticidal properties as well as methods of using the pesticidal compositions to control a pest.
摘要翻译： 本发明涉及一种苏云金芽孢杆菌的新菌株，其中所述菌株的所有杀虫活性基本上都在所述菌株的发酵上清液中。 该菌株产生一种具有抗鞘翅目昆虫害虫活性的物质，并增强了与芽孢杆菌有关的农药的杀虫活性。 本发明还涉及包含该物质和杀虫载体的农药组合物，或该物质和一种与芽孢杆菌有关的农药，化学农药和/或具有杀虫性质的病毒以及使用该杀虫组合物来控制有害生物的方法。
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公开(公告)号：US5139945A
公开(公告)日：1992-08-18
申请号：US245275
申请日：1988-09-16
申请人： Chi-Li Liu
发明人： Chi-Li Liu
CPC分类号： C12R1/07 , C02F1/50 , C12N9/88 , C02F2103/023 , Y10S435/832
摘要： A method of producing algal cell wall degrading enzyme by cultivating a thermophilic, bacterial strain of an atypical Bacillus stearothermophilus e.g., W36-7-4 (NRRL B-18394) which produces the alginate lyase in a cultivation medium containing sodium alginate and tryptone, then allowing growth to take place to accumulate alginate lyase, and then recovering the enzyme from the fermentation broth. The novel alginate lyase of this invention is useful as a treatment agent for water cooling systems.
摘要翻译： 通过培养嗜热芽孢杆菌嗜热芽孢杆菌例如W36-7-4（NRRL B-18394）的嗜热细菌菌株在含有藻酸钠和胰蛋白胨的培养基中产生藻酸盐裂解酶的方法来生产藻细胞壁降解酶， 允许生长以产生藻酸盐裂解酶，然后从发酵液中回收酶。 本发明的新型藻酸盐裂解酶可用作水冷却系统的处理剂。
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公开(公告)号：US08557976B2
公开(公告)日：2013-10-15
申请号：US12366115
申请日：2009-02-05
申请人： Chi-Li Liu , Travis Aaron Mahan , Edward Farley
发明人： Chi-Li Liu , Travis Aaron Mahan , Edward Farley
IPC分类号： C13K13/00
CPC分类号： C12P39/00 , A62D3/02 , A62D2101/22 , C02F3/341 , C02F2101/36 , C12N1/20
摘要： Isolated microbial consortia capable of degrading chlorinated carbohydrates and a method to acclimatize microbes to degrade chlorinated carbohydrates under specific conditions of temperature and salt are described. Also described is a method for using microbial consortia to degrade chlorinated carbohydrates in a waste stream.
摘要翻译： 描述了能够降解氯化碳水化合物的分离的微生物联合体和在温度和盐的特定条件下使微生物驯化以降解氯化碳水化合物的方法。 还描述了使用微生物菌落来降解废物流中的氯化碳水化合物的方法。
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公开(公告)号：US20110014669A1
公开(公告)日：2011-01-20
申请号：US12692943
申请日：2010-01-25
申请人： Kevin T. Madden , David M. Young , Stanley Bower , Chi-Li Liu
发明人： Kevin T. Madden , David M. Young , Stanley Bower , Chi-Li Liu
摘要： A biological method for the conversion of L-glutamate to 1,4-butanediol that involves a decarboxylation step and avoids production of 4-hydroxybutyrate as an intermediate is described. The method includes: (a) conversion of L-glutamate to L-glutamate 5-phosphate; (b) conversion of L-glutamate 5-phosphate to L-glutamate 5-semialdehyde; (c) conversion of L-glutamate 5-semialdehyde to 5-hydroxy-L-norvaline; (d) conversion of 5-hydroxy-L-norvaline to 5-hydroxy-2-oxopentanoate; (e) conversion of 5-hydroxy-2-oxopentanoate to 4-hydroxybutanal; and (f) the conversion of 4-hydroxybutanal to 1,4-butanediol.
摘要翻译： 描述了将涉及脱羧步骤并避免生成4-羟基丁酸酯作为中间体的L-谷氨酸转化为1,4-丁二醇的生物学方法。 该方法包括：（a）将L-谷氨酸转化为L-谷氨酸5-磷酸; （b）将L-谷氨酸5-磷酸转化为L-谷氨酸5-半醛; （c）将L-谷氨酸5-半醛转化为5-羟基-L-正缬氨酸; （d）将5-羟基-L-正缬氨酸转化为5-羟基-2-氧代戊酸酯; （e）将5-羟基-2-氧代戊酸转化为4-羟基丁醛; 和（f）将4-羟基丁醛转化为1,4-丁二醇。
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公开(公告)号：US20070172931A1
公开(公告)日：2007-07-26
申请号：US11339306
申请日：2006-01-25
申请人： Michael Harrison , James Purdue , Penelope Patton , Andrew Hoffman , James Gaddy , Chi-Li Liu , Robert Schanefelt
发明人： Michael Harrison , James Purdue , Penelope Patton , Andrew Hoffman , James Gaddy , Chi-Li Liu , Robert Schanefelt
CPC分类号： C12P19/04
摘要： A process for preparing saccharide oligomers uses an aqueous feed composition that comprises at least one monosaccharide or linear saccharide oligomer, and has a solids concentration of at least about 70% by weight. The feed composition is heated to a temperature of at least about 40° C., and is contacted with at least one catalyst that accelerates the rate of cleavage or formation of glucosyl bonds, such as enzyme or acid, for a time sufficient to cause formation of non-linear saccharide oligomers. A product composition is produced that contains a higher concentration of non-linear saccharide oligomers than linear saccharide oligomers.
摘要翻译： 制备糖低聚物的方法使用包含至少一种单糖或线性糖低聚物并且具有至少约70重量％的固体浓度的含水进料组合物。 将饲料组合物加热至至少约40℃的温度，并与至少一种加速切割或形成葡糖基的速率如酶或酸的催化剂接触足以引起形成的时间 的非线性糖低聚物。 制备的产品组合物含有比线性糖低聚物更高浓度的非线性糖低聚物。
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公开(公告)号：US5976563A
公开(公告)日：1999-11-02
申请号：US946895
申请日：1997-10-08
申请人： Chi-Li Liu , Anita M. MacMullan , Patricia Ann Lufburrow , Robert L. Starnes , Denise C. Manker
发明人： Chi-Li Liu , Anita M. MacMullan , Patricia Ann Lufburrow , Robert L. Starnes , Denise C. Manker
IPC分类号： A01N25/02
CPC分类号： A01N25/02
摘要： The invention is related to a novel strain(s) of Bacillus thuringiensis in which essentially all of the pesticidal activity of said strain is in the supernatant of a fermentation of said strain. The strain produces a substance which has activity against an insect pest(s) of the order Coleoptera and which enhances the pesticidal activity of a Bacillus related pesticide. The invention further relates to pesticidal compositions comprising the substance and a pesticidal carrier, or the substance and a Bacillus related pesticide, a chemical pesticide and/or a virus with pesticidal properties as well as methods of using the pesticidal compositions to control a pest.
摘要翻译： 本发明涉及一种苏云金芽孢杆菌的新菌株，其中所述菌株的所有杀虫活性基本上都在所述菌株的发酵上清液中。 该菌株产生一种具有抗鞘翅目昆虫害虫的活性的物质，其增强了与芽孢杆菌相关的农药的杀虫活性。 本发明还涉及包含该物质和杀虫载体的农药组合物，或该物质和一种与芽孢杆菌有关的农药，化学农药和/或具有杀虫性质的病毒以及使用该杀虫组合物来控制有害生物的方法。
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