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1.发明授权Apparatus for the large scale growth and packaging of cell suspensions and three-dimensional tissue cultures 失效用于大规模生长和包装细胞悬浮液和三维组织培养物的装置
公开(公告)号：US5763267A
公开(公告)日：1998-06-09
申请号：US632972
申请日：1996-04-16
申请人： Christine Kurjan , Mark A. Applegate , James H. Flatt , Dawn Orton Applegate , Nicole Bloom , Mark Baumgartner
发明人： Christine Kurjan , Mark A. Applegate , James H. Flatt , Dawn Orton Applegate , Nicole Bloom , Mark Baumgartner
摘要： An apparatus for the large scale culturing and packaging of cell suspensions, three dimensional tissue, and other biological systems is disclosed. The apparatus includes a plurality of flexible or semi-flexible treatment chambers comprising one or more individual culture pockets, a plurality of rigid spacers, an inlet fluid manifold, an outlet fluid manifold, a fluid reservoir, and a means for transporting fluid within the system. During treatment, liquid media is transported from the fluid reservoir to the inlet manifold, which will in turn evenly distribute the media to each of the connected treatment chambers and internal culture pockets. An outlet fluid manifold is also provided to ensure that each treatment chamber is evenly filled and to ensure that any air bubbles formed during treatment are removed from the treatment chambers. The treatment chambers are flexible or semi-flexible so as to provide for easy end-user handling during rinsing and application of the cultured transplants. Due to the flexibility of the treatment chambers, rigid spacers are also provided which ensure even fluid distribution within the chambers during treatment.
摘要翻译： 公开了用于大规模培养和包装细胞悬浮液，三维组织和其他生物系统的装置。 该装置包括多个柔性或半柔性治疗室，其包括一个或多个单个培养袋，多个刚性隔离物，入口流体歧管，出口流体歧管，流体储存器和用于在系统内输送流体的装置 。 在处理期间，液体介质从流体储存器输送到入口歧管，这将进而将介质均匀地分配到每个连接的处理室和内部培养袋中。 还提供出口流体歧管以确保每个处理室均匀地填充并且确保处理期间形成的任何气泡从处理室中移除。 处理室是柔性的或半柔性的，以便在冲洗和应用培养移植物期间提供容易的最终使用者处理。 由于处理室的灵活性，还提供了刚性间隔件，其确保处理期间室内均匀的流体分布。
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2.发明授权Apparatus and method for simulating in vivo conditions while seeding and culturing three-dimensional tissue constructs 失效用于在种植和培养三维组织构建体时模拟体内条件的装置和方法
公开(公告)号：US6121042A
公开(公告)日：2000-09-19
申请号：US1609
申请日：1997-12-31
申请人： Alvin Peterson , Lee K. Landeen , John Bennett , Jason Gee , Scott Chesla , Joan Zeltinger , James H. Flatt , Mark A. Applegate , Noushin Dunkelman , Stephen V. Kemmerrer
发明人： Alvin Peterson , Lee K. Landeen , John Bennett , Jason Gee , Scott Chesla , Joan Zeltinger , James H. Flatt , Mark A. Applegate , Noushin Dunkelman , Stephen V. Kemmerrer
CPC分类号： C12M35/04 , C12M41/00 , A61F2/30756
摘要： An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing three-dimensional tissue constructs is disclosed. The apparatus includes a fluid reservoir, a pump, at least one treatment chamber, and a means for controlling media flow characteristics around a tissue construct disposed within the treatment chamber, and for controlling movement of the construct itself, so as to simulate a variety of physiologic conditions. One exemplary embodiment of the invention includes a means for applying an axial stress to the construct. Applying an axial stress to the construct during seeding and culturing results in a tissue-engineered construct with cells and their fibers oriented in a manner which is more likely to possess long term dimensional stability and the patency of native vessels with normal physiologic function.
摘要翻译： 公开了用于灭菌，播种，培养，储存，运送和测试三维组织结构的装置和方法。 该装置包括流体储存器，泵，至少一个处理室，以及用于控制设置在处理室内的组织结构周围的介质流动特性的装置，并且用于控制构造体本身的移动，以便模拟各种 生理条件。 本发明的一个示例性实施例包括用于向结构施加轴向应力的装置。 在播种和培养过程中对构建体施加轴向应力导致组织工程构建体中细胞及其纤维以更可能具有长期尺寸稳定性和具有正常生理功能的天然血管通畅的方式取向。
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3.发明授权Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing replacement cartilage tissue constructs 失效灭菌，播种，培养，储存，运输和检测置换软骨组织构造的装置和方法
公开(公告)号：US6060306A
公开(公告)日：2000-05-09
申请号：US912948
申请日：1997-08-14
CPC分类号： C12M23/34 , A61F2/0095 , A61F2/30756 , A61F2/468 , A61L27/38 , C12M21/08 , C12M25/14 , C12M41/40 , C12M41/44 , A61F2002/30062 , A61F2002/30762 , A61F2210/0004
摘要： An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing cartilage constructs is disclosed. The apparatus generally includes a treatment chamber and a means for varying the fluid flow and pressure within the treatment chamber during seeding and culturing. The application of pressure to the cartilage construct improves both the uniformity of seeding throughout the construct and facilitates the flow of nutrients to and removal of waste products from cells embedded in the construct. In this manner, a tissue-engineered cartilage construct with cells and their fibers oriented in a manner which is more likely to possess long term dimensional stability and the patency of cartilage with normal physiologic function may be formed.
摘要翻译： 公开了用于消毒，播种，培养，储存，运送和测试软骨构建体的装置和方法。 该设备通常包括处理室和用于在播种和培养期间改变处理室内的流体流动和压力的装置。 对软骨构造施加压力可改善整个构建体的接种的均匀性，并促进营养物质流入和从植物体内嵌入的细胞中除去废物。 以这种方式，可以形成具有以更可能具有长期尺寸稳定性和具有正常生理功能的软骨通畅的方式取向的细胞及其纤维的组织工程化软骨构建体。
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公开(公告)号：US07960524B2
公开(公告)日：2011-06-14
申请号：US11689596
申请日：2007-03-22
CPC分类号： C12N15/52 , C07K14/28 , C07K14/405 , C12N15/8247 , C12N15/8273 , C12P7/625 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
摘要翻译： 本发明通常涉及从分离自非细菌生物体或衍生自非细菌生物体的多不饱和脂肪酸（PUFA）聚酮化合物合酶（PKS）系统，其同源物，分离的核酸分子和编码这种PUFA PKS的生物活性结构域的重组核酸分子 系统，涉及包含PUFA PKS系统的转基因生物，制备和使用此类体系用于生产感兴趣的生物活性分子的方法，以及鉴定具有这种PUFA PKS系统的新细菌和非细菌微生物的新方法。
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公开(公告)号：US07919320B2
公开(公告)日：2011-04-05
申请号：US11777277
申请日：2007-07-12
CPC分类号： C12N15/52 , C07K14/28 , C07K14/405 , C12N15/8247 , C12N15/8273 , C12P7/625 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
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公开(公告)号：US07662597B2
公开(公告)日：2010-02-16
申请号：US11778590
申请日：2007-07-16
CPC分类号： C12N15/52 , A23D7/001 , A23L33/115 , A23L33/135 , C07K14/28 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
摘要翻译： 本发明一般涉及多不饱和脂肪酸（PUFA）聚酮化合物合酶（PKS）系统，其同源物分离到分离的核酸分子和编码这种PUFA PKS系统的生物活性结构域的重组核酸分子，所述基因修饰的生物体包含PUFA PKS 系统，制备和使用这些系统用于生产感兴趣的生物活性分子的方法，以及用于鉴定具有这种PUFA PKS系统的新的细菌和非细菌微生物的新方法。
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公开(公告)号：US07645598B2
公开(公告)日：2010-01-12
申请号：US11778617
申请日：2007-07-16
CPC分类号： C12N15/52 , A23D7/001 , A23L33/115 , A23L33/135 , C07K14/28 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
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公开(公告)号：US20100099154A1
公开(公告)日：2010-04-22
申请号：US11778594
申请日：2007-07-16
CPC分类号： C12N15/52 , A23D7/001 , A23L33/115 , A23L33/135 , C07K14/28 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
摘要翻译： 本发明一般涉及多不饱和脂肪酸（PUFA）聚酮化合物合酶（PKS）系统，其同源物分离到分离的核酸分子和编码这种PUFA PKS系统的生物活性结构域的重组核酸分子，所述基因修饰的生物体包含PUFA PKS 系统，制备和使用这些系统用于生产感兴趣的生物活性分子的方法，以及用于鉴定具有这种PUFA PKS系统的新的细菌和非细菌微生物的新方法。
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公开(公告)号：US20100024050A1
公开(公告)日：2010-01-28
申请号：US11777277
申请日：2007-07-12
IPC分类号： A01K67/027 , C12N15/11 , C12N15/00 , C12N1/21 , A01H5/00 , C12Q1/02 , C12P7/64 , C12N15/82 , C12N15/87
CPC分类号： C12N15/52 , C07K14/28 , C07K14/405 , C12N15/8247 , C12N15/8273 , C12P7/625 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
摘要翻译： 本发明通常涉及从分离自非细菌生物体或衍生自非细菌生物体的多不饱和脂肪酸（PUFA）聚酮化合物合酶（PKS）系统，其同源物，分离的核酸分子和编码这种PUFA PKS的生物活性结构域的重组核酸分子 系统，涉及包含PUFA PKS系统的转基因生物，制备和使用此类体系用于生产感兴趣的生物活性分子的方法，以及鉴定具有这种PUFA PKS系统的新细菌和非细菌微生物的新方法。
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公开(公告)号：US07638315B2
公开(公告)日：2009-12-29
申请号：US11778614
申请日：2007-07-16
IPC分类号： C12N15/52 , C12P7/64 , C12N15/54 , C12N15/60 , C12N15/74 , C12N15/79 , C12N15/82 , C12N15/53
CPC分类号： C12N15/52 , A23D7/001 , A23L33/115 , A23L33/135 , C07K14/28 , C12P7/6427 , C12P7/6472
摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.
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