Process for the lysis of a culture of lactic acid bacteria by means of a
lysin, and uses of the resulting lysed culture
    9.
    发明授权
    Process for the lysis of a culture of lactic acid bacteria by means of a lysin, and uses of the resulting lysed culture 失效
    通过溶素裂解乳酸菌的培养的方法,以及所得裂解培养物的用途

    公开(公告)号:US5955258A

    公开(公告)日:1999-09-21

    申请号:US737716

    申请日:1997-04-22

    摘要: The invention provides a process for the lysis of a culture of lactic acid bacteria, or a product containing such culture e.g. cheese, by means of a lysin through the in situ production of a homologous autolysin, or a heterologous autolysin obtainable from Gram-positive bacteria esp. from lactic acid bacteria. The gene encoding said autolysin is controlled by a promoter, preferably regulated by food-grade ingredients or parameters, to achieve an enhanced lysis after induction resulting in an enhanced production of total autolysin compared with the natural production lever of the homologous autolysin during fermentation or shortly thereafter. Other uses of the invention include preparing a mixture of peptides which are modified by peptidases freed after the lysis, using the autolysin as a bactericidal agent against spoiling bacteria or pathogenic bacteria for improving the shelf life of a product containing the lysed culture.

    摘要翻译: PCT No.PCT / NL95 / 00170 Sec。 371日期1997年04月22日 102(e)日期1997年4月22日PCT提交1995年5月12日PCT公布。 WO95 / 31561 PCT出版物 日期:1995年11月23日本发明提供了乳酸菌培养物的裂解方法,或含有这种培养物的产物。 干酪,通过原位产生同源自溶素的溶素,或可从革兰氏阳性菌esp获得的异源自溶素。 来自乳酸菌。 编码所述自溶素的基因由优选由食品级成分或参数调节的启动子控制,以在诱导后实现增强的裂解,从而与发酵期间的同源自溶素的天然生产杠杆相比较,或者短期内 之后 本发明的其它用途包括制备由裂解后释放的肽酶修饰的肽的混合物,使用自溶素作为防止破坏细菌或病原菌的杀菌剂,以改善含有裂解培养物的产品的保质期。

    Attaching substances to microorganisms
    10.
    发明申请
    Attaching substances to microorganisms 有权
    将物质附着于微生物

    公开(公告)号:US20070104724A1

    公开(公告)日:2007-05-10

    申请号:US11590738

    申请日:2006-10-30

    摘要: The invention relates to surface display of proteins on microorganisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian, plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide including a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG. 10 and further includes a method for attaching a proteinaceous substance to the cell wall of a microorganism comprising the use of the attaching peptide.

    摘要翻译: 本发明涉及通过将异源蛋白质靶向和锚定到细胞如酵母,真菌,哺乳动物,植物细胞和细菌的外表面上来微生物上蛋白质的表面显示。 本发明提供一种蛋白质物质,其包含反应性基团和至少一种附着肽,其包括具有对应于图1中列出的共有氨基酸序列的至少一部分的序列的一段氨基酸。 并且还包括将蛋白质物质附着到包含使用附着肽的微生物的细胞壁中的方法。